Owing to the advantages of scientific computation and the data feature support provided by artificial intelligence technology, the theoretical exploration and application research of new computing methods for large generators, one of the most expensive energy equipment in a power system, has become a research hotspot toward solving the bearing limit and operation capacity under abnormal working conditions. Because of many factors affecting the distribution of negative sequence loss and temperature rise that have an extremely complex nonlinear relationship, the traditional calculation and prediction methods of negative sequence conditions cannot suitably reflect the time accumulation effect. Therefore, a prediction method of rotor steady-state negative sequence heating based on radial basis function process neural network is proposed in this paper, and a negative sequence working condition prediction model is established. Accordingly, this study focuses on the study of the relationship among negative sequence heating characteristics, negative sequence component proportion, and transverse slot; additionally, their influence degree, variation relationship, and main principles are further explored that provide a theoretical basis for the design and operation of large generators. As observed from the test results, the steady-state negative sequence condition prediction method based on the improved genetic algorithm radial basis function process neural network features high accuracy; it is a feasible prediction method, specifically for negative sequence conditions of large generators.