An Improved Amylase Assay

Jamieson, Andrew D.; Pruitt, Kenneth M.; Caldwell, Robert C.

doi:10.1177/00220345690480032701

Cited by 80 publications

(26 citation statements)

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“…In all rats, amylase serum levels were assessed by Bernfeld's method 23 as modified by Jamieson et al 24 Serum IL-6 and IL-10 levels were determined by enzyme-linked immunoabsorbent assay (ELISA), using BioSource International Cytoscreen TM kits (BioSource International, Camarillo, California, USA)…”

Section: Amylase and Interleukin Serum Levelsmentioning

confidence: 99%

Inhibition of Cyclooxygenase-2 in Experimental Severe Acute Pancreatitis

Almeida

Jukemura

Coelho

et al. 2006

Clinics

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BACKGROUND:The standard treatment for acute pancreatitis (AP) is still based on supportive care. The search for a new drug that could change the natural history of the disease is a continuing challenge for many researchers. The aim of this study is to evaluate the effect of a cyclooxygenase-2 (COX-2) inhibitor on experimental AP in rats. METHODS:The animals were divided into 2 groups: Group 1 (n = 30)-animals with taurocholate-induced AP treated with parecoxib (40 mg/kg). Group 2 (n = 30)-animals with taurocholate-induced AP that received saline. The COX-2 inhibitor (parecoxib) was injected immediately after AP induction, through the penis dorsal vein. The parameters evaluated were histology, serum levels of amylase, IL-6 and IL-10, and mortality rate. RESULTS: The serum levels of IL-6 and IL-10 in the parecoxibtreated group were lower than the control group. The amylase serum levels and the mortality rate remained unchanged in the treated animals. Histologic morphology also was unaltered, except for fat necrosis, which was higher in parecoxib-treated rats. CONCLUSION: Inhibition of Cox-2 decreases the systemic release of inflammatory cytokines, but has a poor effect on the direct pancreas injury caused by taurocholate.

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Section: Amylase and Interleukin Serum Levelsmentioning

confidence: 99%

Inhibition of Cyclooxygenase-2 in Experimental Severe Acute Pancreatitis

Almeida

Jukemura

Coelho

et al. 2006

Clinics

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“…Hemorrhage, fat necrosis and neutrophilic infi ltration were absent in all AP groups. Table 1 -Effect of hyperthermia on histologic edema, according to SCHMIDT et al (33) showing no difference between the groups GRAPHIC 5 -Effect of hyperthermia on amylase serum levels evaluated by JAMIESON et al (18) method, showing no difference between groups studied (P = 0.5901). AP is a infl ammatory disease whose treatment is predominantly supportive (29) .…”

Section: Resultsmentioning

confidence: 93%

“…Serum amylase levels were assessed by cholorimetric method of BERNFELD (3) and modifi ed by JAMIESON et al (18) .…”

Section: Serum Amylase Levelsmentioning

confidence: 99%

Effect of hyperthermia on experimental acute pancreatitis

Almeida

Jukemura

Sampietre

et al. 2006

Arq. Gastroenterol.

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-Background -Recent studies indicate that hyperthermia can change infl ammatory mechanisms and protect experimental animals from deleterious effects of secretagogue-induced acute pancreatitis. Aim -To evaluate the effects of hyperthermia posttreatment on cerulein-induced acute pancreatitis in rats. Methods -Twenty animals were divided in two groups: group I (n = 10), rats with cerulein-induced acute pancreatitis undergone hyperthermia, and group II (n = 10), animals with cerulein-induced acute pancreatitis that were kept normothermic. In all groups, amylase serum levels, histologic damage, vascular permeability and pancreatic water content were assessed. Acute pancreatitis was induced by administration of two cerulein injections (20 mcg/kg). A single dose of Evans' blue dye was administered along with the second dose of cerulein. All animals also received a subcutaneous injection of saline solution. After this process, animals undergone hyperthermia were heated in a cage with two 100 W lamps. Body temperature was increased to 39.5ºC and maintained at that level for 45 minutes. Normothermia rats were kept at room temperature in a second cage. Results -Control animals had typical edema, serum amylase activity and morphologic changes of this acute pancreatitis model. Hyperthermia post-treatment ameliorated the pancreatic edema, whereas the histologic damage and the serum amylase level remained unchanged. Conclusions -The fi ndings suggest a benefi cial effect of the thermal stress on infl ammatory edema in experimental acute pancreatitis.

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“…The α-amylase activity was measured using the dinitrosalicylic acid (DNS) method [16,17] developed by Bernfeld (1955), improved by Jamieson et al (1969), and adopted for testing α-amylase inhibitory potential [18] using 1% soluble starch as substrate. The test substance was pre-incubated with amylase (100 µL) at room temperature for 20 min before the addition of 100 µL of the substrate solution followed by incubation at 37°C for 10 min.…”

Section: α-Amylase Inhibitory Activitymentioning

confidence: 99%

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Raghuvanshi¹

2017

IJGP

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Objective: Ipomoea reniformis was studied for its traditionally claimed activity, and still, it is unexplored. The plant can be further explored for the study of traditionally claimed unexplored activities, as well as isolation and identification of active constituents may lead to new findings. The objective of our study is to investigate antioxidant activity of the extracts of I. reniformis using in vitro models. The measured quantity of extract of I. reniformis was evaluated for its antioxidant activity as compared with standard by different models. The present investigation may be concluded that the plant I. reniformis is endowed with significantly antioxidant activity due to the presence of phenolics and flavonoids, thereby justifying its use in the indigenous system of medicine. Materials and Methods: I. reniformis was collected, dried, and extracted with different solvents, and finally, five extracts were studied for their total phenolics and flavonoids content followed by determination of antioxidant activity by 1, 1-diphenyl-2-picrylhydrazyl, superoxide radical scavenging, and α-amylase inhibitory activity. Results:The obtained results showed that ethanolic extract contains a higher concentration of phenolics and flavonoids and showed highest antioxidant activity among all the samples. Conclusion: The plant showed the antioxidant activity, and we believe that antioxidant activity of I. reniformis was due to synergistic effect of phytochemicals present in it. Further evidencing the need to conduct studies that can identify the active components responsible for the activity.

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An Improved Amylase Assay

Cited by 80 publications

References 0 publications

Inhibition of Cyclooxygenase-2 in Experimental Severe Acute Pancreatitis

Inhibition of Cyclooxygenase-2 in Experimental Severe Acute Pancreatitis

Effect of hyperthermia on experimental acute pancreatitis

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