An Improved Amylase Assay Using a New Starch Derivative

Hall, Frank F.; Culp, T.W.; Hayakawa, Tetsuo; Ratliff, Charles R.; Hightower, Nicholas C.

doi:10.1093/ajcp/53.5.627

Cited by 37 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The supernatant fluids were analyzed for the fol lowing substances: protein (biuret reaction) [Jcnzano et al" 1986], SCN (ferric nitrate method) [Powell, 1945], free sialic acid (thiobarbituric acid method) [Morgan. 1981], peroxidase (dithionitrobcnzoic acid procedure) [Mánsson-Rahemtulla et al, 1986], ct-amylase (using Calbiochem amylopectin azure) [Hall et al. 1970], lysozyme (using the Calbiochem Micrococcus I ulcus suspensions according to suppli er's instructions), sucrasc (by means of the neocupreine method to measure the amount of reducing sugars formed from sucrose, using O-glucose as standard) [Chaplin, 1986], peptidolytic enzyme(s) (using Nc/-benzoyl-DC-arginyl-/)-nitroanilinc, BAPNA, as substrate), and salivary IgA (by means o f the Kallestad Low Level IgA procedure as described in the test kit insert and in the Kallestad Technical Bulletin; Sanofi Diagnostics, Chaska.…”

Section: Chemical Proceduresmentioning

confidence: 99%

Properties of Whole Saliva and Dental Plaque in Relation to 40-Month Consumption of Chewing Gums Containing Xylitol, Sorbitol or Sucrose

Makinen

et al. 1996

Caries Res

View full text Add to dashboard Cite

Samples of whole saliva and dental plaque were collected from initially 10-year old subjects who participated in a 40-month cohort study investigating the effect of chewing gum usage on caries rates. The subjects represented nine cohorts of which one did not receive gum, while in eight cohorts the subjects received gum containing either xylitol, sorbitol, their mixtures, or sucrose as bulk sweeteners, the maximum sweetener consumption in the form of gums being up to 10.7 g/day, used in 3–5 daily chewing episodes. Gum usage had no significant effect on the levels of salivary protein, IgA, α-amylase, peroxidase, lysozyme, SCN- and buffer capacity. At the endpoint, the group that received 100% xylitol pellet-shaped gum five times/day, had significantly lower levels of sucrase (p < 0.05) and free sialic acid (p < 0.001) in whole saliva than at baseline. This group showed significantly (p < 0.05) smaller plaque index scores at two cross-sectional measurements, and exhibited the lowest log₁₀ counts of salivary lactobacilli at endpoint than most other groups. The salivary levels of peptidase(s) (oligopeptidase B-like enzymes) hydrolyzing Nα-benzoyl-DL-arginyl-p-nitroaniline were significantly (p < 0.05) or almost significantly lower in groups which received 100% xylitol pellet gums. All groups exhibited obviously an aging-related increase of salivary mutans streptococcus scores, except the above xylitol group in which the mean scores did not change.

show abstract

Section: Chemical Proceduresmentioning

confidence: 99%

Properties of Whole Saliva and Dental Plaque in Relation to 40-Month Consumption of Chewing Gums Containing Xylitol, Sorbitol or Sucrose

Makinen

et al. 1996

Caries Res

View full text Add to dashboard Cite

show abstract

“…Determination of pancreatic enzyme activities and DNA and protein contents a-Amylase activity was measured by the method of Hall et al (20) using Remazolbrilliantblue-starch as sub¬ strate and porcine a-amylase (type VII-; Sigma, St Louis, MO) as a standard. Chymotrypsin was assayed after activation of its precursor molecules in the sample homogenates by treatment with 10 mg/1 trypsin (type XI chymotrypsin inactivated; Sigma, St Louis, MO).…”

Section: Tissue Collectionmentioning

confidence: 99%

Modification of pancreatic digestive function by pituitary grafting in mice

Matsuda

Mori

Park

et al. 1995

European Journal of Endocrinology

View full text Add to dashboard Cite

The effect of prolactin on the digestive potency of the acinar pancreas was examined in pituitary-grafted hyperprolactinemic mice, because our previous experiment showed that a marked proliferation of pancreatic acinar cells was induced by pituitary grafting in mice. To know whether the digestive function is modified, the tissue contents of pancreatic digestive enzymes, such as chymotrypsin, lipase alpha-amylase and ribonuclease, were measured in the hyperprolactinemic mice. Pituitary grafting significantly increased the contents of chymotrypsin and lipase in the pancreas on day 12 after the operation without affecting intake of food, when compared to those in the sham-operated controls. On day 30, however, the differences between pituitary-grafted and control mice were no more discernible. Thus, the digestive enzyme activities are easily modified soon after the increase of circulating prolactin level. This effect of prolactin on the function of the pancreas may be responsible for "homeorhetic" control of nutrients during lactation. In another set of experiments in adrenalectomized-castrated or castrated mice, pituitary grafting induced an increase in the weight of the pancreas. In addition, adrenalectomy in combination with castration did not alter the pancreatic contents of chymotrypsin and lipase but decreased the amylase content. These results taken together seem to indicate that the effect of prolactin on the exocrine pancreas is not mediated by gonadal and adrenal steroid hormones.

show abstract

“…The mixture was incubated for 5 min at room temperature, and then the residual enzyme activity was determined. The subtilisin activity was measured using N-CBZ-L-Ala-L-AIa-L-Leu p-nitroanilide [11], and the s-amylase activity was evaluated using amylopectin azure as substrate [12].…”

Section: Methodsmentioning

confidence: 99%

Enzymatic oxidation of the bifunctional wheat inhibitor of subtilisin and endogenous α‐amylase

1993

View full text Add to dashboard Cite

Oxidation of the bifunctional wheat inhibitor of subtilisin and endogenous c~-amylase catalyzed by horseradish peroxidase results in the loss of the inhibitory activity against both enzymes. The enzymatic oxidation is accompanied by modification of one methionine and two tryptophan residues in the protein. The results obtained, together with data on chemical modification and limited proteolysis, allow us to conclude that Met 34 Ala 35 is the reactive site of the inhibitor responsible for the interaction with subtilisin. It is supposed that the reactive site of the inhibitor responsible for the interaction with c~-amylase contains one or two tryptophan residues.

show abstract

An Improved Amylase Assay Using a New Starch Derivative

Cited by 37 publications

References 0 publications

Properties of Whole Saliva and Dental Plaque in Relation to 40-Month Consumption of Chewing Gums Containing Xylitol, Sorbitol or Sucrose

Properties of Whole Saliva and Dental Plaque in Relation to 40-Month Consumption of Chewing Gums Containing Xylitol, Sorbitol or Sucrose

Modification of pancreatic digestive function by pituitary grafting in mice

Enzymatic oxidation of the bifunctional wheat inhibitor of subtilisin and endogenous α‐amylase

Contact Info

Product

Resources

About