An improved autoanalyzer technique for the determination of serum alkaline phosphatase

Price, Christopher P.; Woodman, Dorothy

doi:10.1016/0009-8981(71)90192-6

Cited by 10 publications

(4 citation statements)

References 3 publications

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“…Plasma alkaline phosphatase (ALP) (EC 3.1.3.1) activity was determined according to Price and Woodman (1971). Twenty microliters of plasma samples were incubated with nitro‐4‐phenylphosphate as substrate of the enzyme at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Effect of age‐dependent exposure to lead on hepatotoxicity and nephrotoxicity in male rats

Berrahal

Lasram

Elj

et al. 2011

Environmental Toxicology

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Lead is known to induce a broad range of physiological, biochemical, and behavioral dysfunctions in laboratory animals and humans. This includes age-specific variations in absorption, retention, and tissue distribution of lead. This study was carried out to investigate the effects of chronic exposure to lead (50 mg/L) on liver and kidneys of two different age groups of male rats treated with lead from delivery until puberty period (40 days) and postpuberty period (65 days). For this purpose, the concentrations of thiobarbituric acid reactive substance (TBARS), total thiol groups (SH), and superoxide dismutase (SOD) activity were measured in the liver and kidney of rats. Renal function was analyzed by determining creatinine, acid uric, and urea. Plasma activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and albumin were determined spectrophotometrically to evaluate hepatic function. These markers of damage were determined to assess the level of toxicity in these animals. Our results clearly show that the administration of lead produces oxidative damage in liver and kidney, as strongly suggested by the significant increase in TBARS, decrease in total SH, and the alteration of SOD activity. In young lead-exposed animals, lead-induced perturbations on the synthetic function of the liver and the kidney were more pronounced. However, nephropathy is evident for adult lead-exposed animals. It is concluded that lead induces severe hepatic and renal toxicity, which depends on the age of the animals and the target organ.

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Section: Methodsmentioning

confidence: 99%

Effect of age‐dependent exposure to lead on hepatotoxicity and nephrotoxicity in male rats

Berrahal

Lasram

Elj

et al. 2011

Environmental Toxicology

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“…Serum alkaline phosphatase activity was determined by means of an AutoAnalyzer technique employing disodium phenyl phosphate as substrate (Price and Woodman, 1971).…”

Section: Methodsmentioning

confidence: 99%

An interpretation of the serum alkaline phosphatase isoenzyme patterns in patients with obstructive liver disease.

Price¹,

Sammons²

1976

J Clin Pathol

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Earlier studies have identified two main isoenzymes of alkaline phosphatase in the sera of patients with obstructive liver disease. This paper reports on a study of these isoenzymes in specific types of liver disease where the pathology in relation to bile duct obstruction is known. The results have been used to support the theory that in biliary obstruction the increase in serum alkaline phosphatase is in part due to regurgitation of the biliary isoenzymes.

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“…Ten pooled sera (mean activity 30.8, range 15.2 to 54.5 King-Armstrong units/100 ml) were assayed using the method of Belfield and Goldberg (1971) at 4 substrate concentrations in the presence and absence of magnesium (l mmol/I), The activities were expressed as the % age of their activity at 18 mmoljl phenyl phosphate concentration (the highest concentration tested) in the presence of magnesium (Fig. There was only slightly greater activity at 18 rnmol/l, recommended by Fishman and Ghosh (1967) and Henry et al (1974), than at 10 mmol/l, recommended by BuchandBuch(l939) and Price and Woodman (1971). There was only slightly greater activity at 18 rnmol/l, recommended by Fishman and Ghosh (1967) and Henry et al (1974), than at 10 mmol/l, recommended by BuchandBuch(l939) and Price and Woodman (1971).…”

Section: Effect Of Various Factors On Alkaline Phosphatase Activity Wmentioning

confidence: 98%

Quality Control of Serum Alkaline Phosphatase Assays: Project Report and Discussion of Some Factors Affecting the Assay

Ellis

1976

Ann Clin Biochem

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The factors which affect the standardisation and quality control of serum alkaline phosphatase assays are discussed. A quality control project was designed to test the performance of seven Birmingham laboratories in the assay of sera with alkaline phosphatase activities outside the range normally tested by the National Quality Control and Wellcome schemes. The results showed that the precision of individual laboratories was satisfactory. Differences between the results of the laboratories were considerable and could be accounted for by differences in methodology. Auto-Analyzer methods employing phenyl phosphate as substrate would best be standardised by adopting the optimised reaction conditions of Buch and Buch (1939); but the borate buffer of these authors should be replaced by the carbonate-bicarbonate buffer of Moss et al. (1971). To avoid the confusion which may arise in future if alkaline phosphatases are reported in U/l irrespective of the substrate, it is suggested that some substrate--indicative nomenclature may be advisable.

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An improved autoanalyzer technique for the determination of serum alkaline phosphatase

Cited by 10 publications

References 3 publications

Effect of age‐dependent exposure to lead on hepatotoxicity and nephrotoxicity in male rats

Effect of age‐dependent exposure to lead on hepatotoxicity and nephrotoxicity in male rats

An interpretation of the serum alkaline phosphatase isoenzyme patterns in patients with obstructive liver disease.

Quality Control of Serum Alkaline Phosphatase Assays: Project Report and Discussion of Some Factors Affecting the Assay

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