An improved brine shrimp larvae lethality microwell test method

Zhang, Yi; Mu, Jun; Han, Jinyuan; Gu, Xiao-Jie

doi:10.3109/15376516.2011.583297

Cited by 21 publications

(15 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The larvae (I instar) of A. salina hatched out as described by Harwig and Scott [33]. For the toxicity test, varying concentrations of 40, 60, 80, 100, 120, 140, and 160 μ L mL −1 of the NPs solution were transferred to the 24-well plates containing 1 mL of sterilized seawater [34]. The live nauplii of A. salina (10 nauplii per well) were added to it and incubated at 25°C.…”

Section: Methodsmentioning

confidence: 99%

Synthesis and Characterization of Silver and Gold Nanoparticles Using Aqueous Extract of Seaweed,Turbinaria conoides,and Their Antimicrofouling Activity

Ramkumar

Prakash

Singamuthu

et al. 2014

The Scientific World Journal

195

View full text Add to dashboard Cite

Silver and gold nanoparticles were synthesized using an aqueous extract of the seaweed Turbinaria conoides and their antibiofilm activity against marine biofilm forming bacteria is reported here. The UV-Vis spectra showed the characteristics SPR absorption band for Ag NPs at 421 and for Au NPs at 538 nm. Further, the synthesized nanoparticles were characterized using FT-IR, XRD, FESEM, EDX, and HRTEM analysis. Spherical and triangular nanostructures of the Ag and Au nanoparticles were observed between the size ranges of 2–17 nm and 2–19 nm, respectively. The synthesized Ag NPs are efficient in controlling the bacterial biofilm formation; however, Au NPs did not show any remarkable antibiofilm activity. The maximum zone of inhibition was recorded against E. coli (17.6 ± 0.42 mm), followed by Salmonella sp., S. liquefaciens, and A. hydrophila. The macrotube dilution method inferred the MIC (20–40 µL mL−1) and MBC (40–60 µL mL−1) of Ag NPs. The CLSM images clearly showed the weak adherence and disintegrating biofilm formation of marine biofilm bacterial strains treated with Ag NPs. The Artemia cytotoxicity assay recorded the LC50 value of 88.914 ± 5.04 µL mL−1. Thus the present study proved the efficiency of Ag NPs as a potent antimicrofouling agent and became the future perspective for the possible usage in the biofouling related issues in the aquaculture installations and other marine systems.

show abstract

Section: Methodsmentioning

confidence: 99%

Synthesis and Characterization of Silver and Gold Nanoparticles Using Aqueous Extract of Seaweed,Turbinaria conoides,and Their Antimicrofouling Activity

Ramkumar

Prakash

Singamuthu

et al. 2014

The Scientific World Journal

195

View full text Add to dashboard Cite

show abstract

“…The test was performed as previously described (Zhang et al, 2012). The samples were tested for their lethality in 96-well microwell plates.…”

Section: Cytotoxicity Against Brine Shrimp Larvamentioning

confidence: 99%

Isolation and characterization of bioactive fungi from shark Carcharodon carcharias’ gill with biopharmaceutical prospects

Zhang

Han

Feng

et al. 2015

Chin. J. Ocean. Limnol.

Self Cite

View full text Add to dashboard Cite

Until recently, little was known about the fungi found in shark gills and their biomedicinal potential. In this article, we described the isolation, bioactivity, diversity, and secondary metabolites of bioactive fungi from the gill of a shark ( Carcharodon carcharias ). A total of 115 isolates were obtained and grown in 12 culture media. Fifty-eight of these isolates demonstrated signifi cant activity in four antimicrobial, pesticidal, and cytotoxic bioassay models. Four randomly selected bioactive isolates inhibited human cancer cell proliferation during re-screening. These active isolates were segregated into 6 genera using the internal transcribed spacer-large subunit (ITS-LSU) rDNA-sequence BLAST comparison. Four genera, Penicillium , Aspergillus , Mucor , and Chaetomium were the dominant taxa. A phylogenic tree illustrated their intergenera and intragenera genetic diversity. HPLC-DAD-HRMS analysis and subsequent database searching revealed that nine representative strains produced diverse bioactive compound profi les. These results detail the broad range of bioactive fungi found in a shark's gills, revealing their biopharmaceutical potential. To the best of our knowledge, this is the fi rst study characterizing shark gill fungi and their bioactivity.

show abstract

“…It also can be used as a preliminary screening model for agricultural pesticides. The experimental method used was similar to that in our previous study [5]. In the test, the concentration gradients were set to be 1.024, 0.512, 0.256, 0.128, 0.064, 0.032, 0.016, 0.008, 0.004, 0.002, 0.001, and 0.0005 mg mL 1 .…”

Section: Brine Shrimp Lethality Testmentioning

confidence: 99%

“…In the test, the concentration gradients were set to be 1.024, 0.512, 0.256, 0.128, 0.064, 0.032, 0.016, 0.008, 0.004, 0.002, 0.001, and 0.0005 mg mL 1 . Then, the semi-lethal concentrations (LC 50 ) were calculated from the lethal curve fit by polynomial regressions [5].…”

Section: Brine Shrimp Lethality Testmentioning

confidence: 99%

Bioactivity and constituents of several common seaweeds

Zhang

Han

et al. 2013

Chin. Sci. Bull.

Self Cite

View full text Add to dashboard Cite

Bioactivity and constituents of 8 common seaweeds from Dalian intertidal zone of northern Yellow Sea were investigated. In the anti-methicillin-resistant Staphylococcus aureus (MRSA) test, Symphyocladia latiuscula and Enteromorpha intestinalis showed obvious activities with MICs much lower than 1.0 mg mL 1 . In the DNA damage repair test (DDRT), Chondrus ocellatu showed selective inhibitory activity against the DNA repair-defective E. coli strain vs. the wild-type E. coli strain; while Sym. latiuscula, Enteromorpha intestinalis and Sar. kjellmanianum showed significant anti-E. coli activity with MICs of 64-128 g mL 1 . In the anti-Pyricularia oryzae test, Sym. latiuscula and Rh. confervoides strongly inhibited the germination of the spores of P. oryzae on agar plate. In the brine shrimp larvicidal test, Sym. latiuscula, Rh. confervoides and Sar. kjellmanianum exhibited potent toxicity against brine shrimp larvae, with LC 50 much lower than 1 mg mL 1 . The HPTLC analysis revealed their diversified secondary metabolites. The HPLC-DAD-MS analysis of the strongest species Sym. latiuscula and database searching showed that it can produce quite diversified metabolites, including halogenated ones, some of which may be new natural products. The results demonstrated the potentials of these seaweeds in the development of new antibiotics, antitumor drugs, agricultural fungicides and pesticides. Macroalgae are important primary producers of marine and coastal ecosystem and are also useful medicine and pesticide resources due to their potentials in accumulating large quantities of bioactive metabolites [1]. As is known to all, algal diversity and biomass depend on the environmental factors such as temperature, light, and nutrition, which can be influenced by human activities (e.g. introducing species and pollutions in the form of heat and eutrophication). Therefore, it is important to investigate and utilize the algal resources in coastal zones. Dalian, an important harbor and industrial city, is located on the tip of the Liaodong Peninsula, between China's inner Bohai Sea and the north Yellow Sea. Its marine ecological environment is influenced by strong sea currents and rivers, which provides diversified habitats for seaweeds and also brings about human influences. In this article, we evaluated the biomass and their biomedical and agrichemical potentials as new antibiotics, antitumor drugs, agricultural fungicides, and pesticides in some common seaweeds from Dalian coastline by determining several bioactivities of them, including anti-methicillin-resistant Staphylococcus aureus (MRSA), anti-Escherichia coli DNA repair-defective/wildtype strains, anti-Pyricularia oryzae, and anti-brine shrimp (Artemia parthenogenetica L.) larvae.

show abstract

An improved brine shrimp larvae lethality microwell test method

Cited by 21 publications

References 19 publications

Synthesis and Characterization of Silver and Gold Nanoparticles Using Aqueous Extract of Seaweed,Turbinaria conoides,and Their Antimicrofouling Activity

Synthesis and Characterization of Silver and Gold Nanoparticles Using Aqueous Extract of Seaweed,Turbinaria conoides,and Their Antimicrofouling Activity

Isolation and characterization of bioactive fungi from shark Carcharodon carcharias’ gill with biopharmaceutical prospects

Bioactivity and constituents of several common seaweeds

Contact Info

Product

Resources

About