This paper investigates the challenging problem of fixed-time formation trajectory tracking control for multiple unmanned surface vessels (USVs) affected by uncertain model dynamics, time-varying external ocean disturbances, as well as input saturation. Firstly, an adaptive super-twisting lumped disturbance observer (ASTLDO) is created by integrating high-order sliding mode with observer technology, which can accurately observe and compensate for the complex disturbance of the system within a finite time. Secondly, following the disturbance observer, backstepping technique, fixed-time control, and virtual leader–follower algorithm, the fixed-time formation tracking strategy is implemented. The proposed formation tracking control scheme enables the multiple surface vessels system to converge and maintain a stable desired formation in a fixed time, and the convergence time is independent of the initial states of the system. Furthermore, an adaptive auxiliary system is introduced to mitigate input saturation. In the end, the effectiveness and anti-interference ability of the suggested approach are confirmed by the formation simulation results of three USVs.