A method that can fast, real-time get the projection matrix in ART reconstruction is proposed based on helical cone-beam CT trajectory in this paper. For a given ray this method can rapidly acquire the indices and corresponding lengths of the voxels passed through by this ray through sorting the intersections between the ray and mesh planes. Meanwhile we only store the projection matrix elements required for a given ray and release them after using them. By doing so the computation time and the storage space are significantly reduced. The experimental results show that this method can heighten the speed and better suppress noise.