An improved fluorimetric method for assay of Dopa in urine and tissues and its use for determination of urinary Dopa, at endogenous level, in different species

Dutruge

et al. 1980

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Catecholamines (DA, NE, E), methoxyamines (MT, NMN, MN), DOPA and DOPAC were studied in urine of term small for gestational age infants (SGA) and preterm with appropriate birthweights for gestational age (PT) during the first ten days of life. Results were compared to values obtained for full term infants (FT). As a whole no deficit in urine catecholamines was observed in either group of SGA and PT neonates suggesting that capacities to synthesize catecholamines are already developed at birth. Furthermore, in SGA infants, adrenergic function seems to be enhanced during the first four days of life; however, SGA infants with low blood glucose levels excreted amounts of epinephrine similar to those of FT neonates, but much lower than those obtained in normoglycemic SGA neonates. These data suggest that enhanced release of catecholamines is required in SGA infants to maintain the glycemic homeostasis. In premature infants, the adrenergic pattern was highly altered only in younger preterm neonates (31 weeks of gestational age) who excreted more catecholamines than older preterm babies (33 to 36 weeks) or full term neonates; this catecholamine increase in urine of young preterm infants might be related to immaturity of storage vesicles and/or to thermoregulatory or respiratory events. On the other hand, a striking deficit in excretion of DOPAC was observed in small for gestational age infants and in young preterm neonates during the first ten days of life. DOPAC excretion was even lower in SGA than in young preterm neonates. These findings suggest that the maturation of dopaminergic neurons occurs late in gestational age and is greatly dependent on nutritional factors.

Section: Methodsmentioning

confidence: 99%

Neonatal pattern of adrenergic metabolites in urine of small for gestational age and preterm infants

Dutruge

et al. 1980

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“…In the final eluates, DOPA, DA, NE and E were assayed by automated fluorimetry of hydroxyindoles derivatives: DOPA at pH 6 (Cottet-Emard and Peyrin, 1977), E at pH 2.8 and NE at pH 6 (Peyrin and Cottet-Emard, 1973), DA at pH 5.2 (Dalmaz and Peyrin, 1976). DOPAC was assayed by continuous flow colorimetry .…”

Section: Urine Samplesmentioning

confidence: 99%

Sex-differences in catecholamine metabolites in human urine during development and at adulthood

1982

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Adrenergic amines (dopamine [DA], norepinephrine [NE], epinephrine [E] and related compounds (DOPA, 3-methoxytyramine [MT], normetanephrine [NMN], metanephrine [MN], 3-5-dihydroxyphenylacetic acid [DOPAC], vanilmandelic acid [VMA] were analyzed in urine of human from one day to 40 years of age, in view to investigate the sex influence on catecholamine metabolism during life. In neonatal life (1 day to 3.5 months), the total amounts of urinary (E + MN) and (NE + NMN + VMA) were lower in girls than in boys, the former two compounds being mainly of adrenal medullary origin and the latter three coming largely from the sympathetic neurons. In adulthood, women excreted epinephrine (12.5 +/- 2.2 micrograms/24 hours) at lower levels then men (22.8 +/- 4.5 micrograms/24 hours) but no sex related differences occurred in the total amounts of urinary E + MN and NE + NMN + VMA. It is concluded from our study that, during the neonatal life, girls exhibit a lesser adreno-sympathetic maturity than boys, but full maturation of peripheral adrenergic sites is reached near to the fifth year of age in both sexes. The role of testosterone has been discussed. In adults, the lower epinephrine levels found in women urine do not seem to result from a sex difference in the degree of adrenergic maturity, but perhaps partly from an increased rate of epinephrine inactivation by catechol-O-methyltransferase.

“…In the final eluates, DOPA, DA, NE and E were assayed by automated fluorimetry of hydroxyindoles derivatives: DOPA at pH 6 (Cottet-Ernard and Peyrin, 1977); E at pH 2.85 and NE at pH 6 (Peyrin and CottetErnard, 1973) and DA at pH 5.2 ( 'Dalrnaz and Peyrin, 1976). DOPAC was assayed by continuous flow colorimetry (Peyrin et aI., 1978).…”

Section: Urine Samplesmentioning

confidence: 99%

Age-related changes in catecholamine metabolites of human urine from birth to adulthood

Sann

et al. 1979

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Catecholamines (dopamine [DA], norepinephrine [NE], epinephrine [E]), methoxyamines (3-methoxytyramine [MT], normetanephrine [NMN], metanephrine [MN]), DOPA, and acidic metabolites (3,4-dihydroxyphenylacetic acid [DOPAC], vanilmandelic acid [VMA]) were determined in human urines from one day of age to adulthood, in order to investigate sympatho-adrenal development during life. All adrenergic compounds are present in neonate urines on the first day of life, but their postnatal evolution is quite different according to the nature of metabolites. Daily E, MN and VMA amounts remain low until the 10th month of life; daily NE, MT and DOPA levels increase progressively, but, in contrast, NMN amounts are already high in the neonatal period and increase only beyond the fourth year of age. DA is at either age the predominant catecholamine but its elimination is relatively more important in the neonatal period.