In this paper, we make a detailed discussion on the η-meson leading-twist light-cone distribution amplitude (LCDA) φ 2;η (u, µ) by using the QCD sum rules approach under the background field theory. Taking both the non-perturbative condensates up to dimension-six and the next-to-leading order (NLO) QCD corrections to the perturbative part, its first three moments ξ n 2;η | µ0 with n = (2, 4, 6) can be determined, where the initial scale µ 0 is set as the usual choice of 1 GeV. Numerically, we obtain ξ 2 2;η | µ0 = 0.204 +0.008 −0.012 , ξ 4 2;η | µ0 = 0.092 +0.006 −0.007 , and ξ 6 2;η | µ0 = 0.054 +0.004 −0.005 . Next, we calculate the D s → η transition form factor (TFF) f + (q 2 ) within the QCD light-cone sum rules approach up to NLO level. Its value at the large recoil region is f + (0) = 0.484 +0.039 −0.036 . After extrapolating the TFF to the allowable physical region, we then obtain the total decay widthes and the branching fractions of the semi-leptonic31.197 +5.456 −4.323 ) × 10 −15 GeV, B(D + s → ηe + ν e ) = 2.389 +0.418 −0.331 for D + s → ηe + ν e channel, and Γ(D + s → ηµ + ν µ ) = (30.849 +5.397 −4.273 ) × 10 −15 GeV, B(D + s → ηµ + ν µ ) = 2.362 +0.413 −0.327 for D + s → ηµ + ν µ channel respectively. Those values show good agreement with the recent BES-III measurements.