An improved medium for adventitious shoot formation and callus induction in Beta vulgaris L. in vitro

Abstract

Six sugarbeet (Beta vulgaris L.) lines (GWI-248, SPB-11, MonoHy 55, SMS-1, EL45 and FC607) were tested for regeneration. Shoot cultures were initiated in vitro from naked, sterilized embryos obtained from mature seed. Excised petioles from cultured shoots were plated on Gamborg's B5 medium and four modified Murashige and Skoog (MS) media. A medium containing MS inorganic salts supplemented with 0.4 mg/1 N(6)-benzyladenine, 0.1 mg/1 indole-3-butyric acid, ten vitamins and six amino acids, termed RV, was superior for both adventitious shoot and callus formation. Callus was observed only on RV medium and only on petioles that did not develop adventitious buds directly. Rooting of regenerated shoots and development of complete plants was accomplished by transfer to Gamborg's B5 medium with 5 mg/l indole-3-butyric acid as the sole phytohormone. The complete process of regeneration through adventitious shoot production took from 4 to 6 weeks from explants to rooted plants. The callus that formed on nonorganogenic petioles was regenerative when transferred to fresh RV medium. Regeneration from callus occurred mainly by shoot organogenesis but also by somatic embryogenesis at a low frequency.

Measurement and effects of gel matric potential and expressibility on production of morphogenic callus by cultured sugarbeet leaf discs

“…Gels were prepared using Bacto agar (Difco), Gelrite (Kelco division of Merck), Phytagar (GIBCO) and HGT and SeaPlaque agarose (FMC) in RV medium (Freytag et al 1988) modified to contain 1 mg 1-1 BA (N 6-benzyladenine as the sole growth regulator and 3% sucrose. Media were adjusted to pH 5.8, sterilized by autoclaving at 121°C for 15 min, distributed into Petri dishes (100 x 25 mm; 35 ml/dish) and allowed to solidify for about 21 h at room temperature.…”

Tissue culture response of Beta germplasm: callus induction and plant regeneration

“…Reliable tissue culture techniques provide a necessary link between genetic manipulation and conventional methods of crop improvement. In sugarbeet breeding, however, in vitro approaches have not yet been realized due to limited progress in regeneration of functional plants [1,5,10,14].…”

Formation ofEnt-isophleichrome byCladosporium herbarumIsolated from Sugar Beet

“…Leaf discs were immersed in a suspension of Agrobacterium tumefaciens, as part of a genetic transformation experiment, before plating on a modified Murashige-Skoog medium. This medium contained the ten vitamins and six amino acids of RV medium 3 ) and 1 mg/liter of N 6 -benzyladenine as the only hormone. The antibacterial agents kanamycin and cefotaxime were included, both at concentrations of 200 mg/liter.…”