An improved method for studying the peristaltic reflex in the isolated colon

Frigo, G. M.; Lecchini, Sergio

doi:10.1111/j.1476-5381.1970.tb12898.x

Cited by 65 publications

(35 citation statements)

References 23 publications

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“…By analysing the pattern of the reflex in the isolated colon (Frigo & Lecchini, 1970), we can distinguish in the response of the longitudinal muscle coat a phasic contraction, which develops before the start of propulsion, and a tonic contraction, which increases in magnitude as propulsion progresses. Since both phases are blocked by tetrodotoxin and ganglion-blocking drugs, the nerve fibres and the ganglion cells of the intramural plexus are involved.…”

Section: Discussionmentioning

confidence: 99%

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A pharmacological analysis of the peristaltic reflex in the isolated colon of the guinea‐pig or cat

Crema

Frigo

Lecchini

1970

British J Pharmacology

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Summary1. The peristaltic reflex in the colon was elicited by a localized intraluminal stimulus. The contractile response of the longitudinal coat, which consists of two phases, begins before the start of propulsion. Although the contractions of the longitudinal and circular musculature are usually associated, they may be independent of each other. In particular, the longitudinal contraction does not seem to be necessary for propulsion.

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Section: Discussionmentioning

confidence: 99%

“…The experiments were carried out in the guinea-pig and in the cat by the method described in the preceding paper (Frigo & Lecchini, 1970).…”

Section: Methodsmentioning

confidence: 99%

A pharmacological analysis of the peristaltic reflex in the isolated colon of the guinea‐pig or cat

Crema

Frigo

Lecchini

1970

British J Pharmacology

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“…Myenteric neurones are known to contain neural pathways responsible for peristalsis of the intestine (reviewed by Kosterlitz & Lees, 1964;North, 1982). These neuronal pathways have been studied by inflation of a small balloon in the intestine (Frigo & Lecchini, 1970) or by mechanical distension (Costa & Furness, 1976) to produce a localized stimulus. Hirst & McKirdy (1974) demonstrated the presence of a descending neural pathway in the guinea-pig small intestine excited by distension of the intestinal wall or transmural electrical stimulation.…”

Section: Introductionmentioning

confidence: 99%

Differences in control of descending inhibition in the proximal and distal regions of rat colon

Hata

Kataoka

Takeuchi

et al. 1990

British J Pharmacology

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Descending inhibition in the proximal and distal portions of rat colon was studied separately, in vitro. In the proximal colon, localized distension with a small balloon caused three types of response (contraction; relaxation; relaxation, then contraction) of the circular muscle on the anal side of the distended region. Distension caused descending relaxation of circular muscle in all segments of the proximal colon, although for this prostaglandin F2α (PGF2α) was necessary in some segments to increase muscle tone. Atropine and guanethidine did not inhibit this descending relaxation, but tetrodotoxin did. Hexamethonium inhibited the descending relaxation in 14 of 17 preparations of proximal colon tested, but not in the others. In the distal colon, distension consistently caused an increase in the tone of the circular muscles. Descending relaxation was observed only after development of higher tone. Atropine and guanethidine did not inhibit the relaxation, but tetrodotoxin did. Hexamethonium did not inhibit the descending relaxation in most of the preparations of distal colon examined. AF64A, an inhibitor of choline uptake, inhibited the response mediated by cholinergic neurones in vitro to electrical transmural stimulation of the longitudinal muscle of proximal colon. Treatment of colonic preparations with AF64A in vitro resulted in inhibition of descending relaxation in those of proximal, but not those of distal, colon. The participation of intrinsic cholinergic neurones in the descending neuronal pathway is strongly suggested by the results in the proximal colon, but less so in the distal colon. The tone and spontaneous contractile activity of colonic circular muscles are discussed in relation to their neuronal control.

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“…It has been shown that PN stimulation enhances colonic peristalsis in response to balloon distension while sympathetic stimulation inhibits colonic peristalsis of the guinea pig distal colon in vitro. 36,37 However, it remains unclear whether PN stimulation affects fecal pellet propulsion or luminal pressure changes. As shown in Supplementary Video 2, we have clearly demonstrated that PN stimulation accelerates pellet propulsion.…”

Section: Discussionmentioning

confidence: 99%

Autonomic Nerve Regulation of Colonic Peristalsis in Guinea Pigs

Gribovskaja‐Rupp¹,

Babygirija²,

Takahashi³

et al. 2014

J Neurogastroenterol Motil

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Background/AimsColonic peristalsis is mainly regulated via intrinsic neurons in guinea pigs. However, autonomic regulation of colonic motility is poorly understood. We explored a guinea pig model for the study of extrinsic nerve effects on the distal colon. MethodsGuinea pigs were sacrificed, their distal colons isolated, preserving pelvic nerves (PN) and inferior mesenteric ganglia (IMG), and placed in a tissue bath. Fecal pellet propagation was conducted during PN and IMG stimulation at 10 Hz, 0.5 ms and 5 V. Distal colon was connected to a closed circuit system, and colonic motor responses were measured during PN and IMG stimulation. ResultsPN stimulation increased pellet velocity to 24.6 ± 0.7 mm/sec (n = 20), while IMG stimulation decreased it to 2.0 ± 0.2 mm/sec (n = 12), compared to controls (13.0 ± 0.7 mm/sec, P ＜ 0.01). In closed circuit experiments, PN stimulation increased the intraluminal pressure, which was abolished by atropine (10 −6 M) and hexamethonium (10 −4 M). PN stimulation reduced the incidence of non-coordinated contractions induced by NG-nitro-L-arginine methyl ester (L-NAME; 10 −4 M). IMG stimulation attenuated intraluminal pressure increase, which was partially reversed by alpha-2 adrenoceptor antagonist (yohimbine; 10 −6 M). Conclusions

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An improved method for studying the peristaltic reflex in the isolated colon

Cited by 65 publications

References 23 publications

A pharmacological analysis of the peristaltic reflex in the isolated colon of the guinea‐pig or cat

A pharmacological analysis of the peristaltic reflex in the isolated colon of the guinea‐pig or cat

Differences in control of descending inhibition in the proximal and distal regions of rat colon

Autonomic Nerve Regulation of Colonic Peristalsis in Guinea Pigs

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