An improved method for the isolation and quantitative cultivation of embryonic cells

Rinaldini, Luis M.

doi:10.1016/0014-4827(59)90119-3

Cited by 136 publications

(26 citation statements)

References 16 publications

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“…The crystals were purified according to Delafield [35], dissolved and activated by incubation for 3 h at 28 "C in Rinaldini's buffer [36] containing 0.05% trypsin and 10 mM triethanolamine, pH 10.2. Residual spores or undissolved crystals were eliminated by centrifugation (I2000 x g, 10 min, 4 C).…”

Section: Bucterial Strains and Plusmid D N Amentioning

confidence: 99%

Identification and characterization of Heliothis virescens midgut membrane proteins binding Bacillus thuringiensisδ‐endotoxins

Oddou

Hartmann

Geiser

1991

European Journal of Biochemistry

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To investigate the specificity of Bacillus thuringiensis var. kurstaki strain HD1 insecticidal crystal proteins (ICP), we used membrane preparations obtained from the midgut of Heliothis virescens larvae to perform separate ligand-blot experiments with the three activated CryIA toxins. The CrylA(a) and the CrylA(b) toxins bind the same 170-kDa protein, but most likely at two different binding sites. The CryIA(c) toxin binds two proteins of molecular masses 140 kDa and 120 kDa. We also demonstrate that the binding proteins for each of the B. thuringiensis toxins are not part of a covalent complex. Although the 170-kDa protein is a glycoprotein, endoglycosidase treatment does not prevent the binding of the CryIA

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Section: Bucterial Strains and Plusmid D N Amentioning

confidence: 99%

Identification and characterization of Heliothis virescens midgut membrane proteins binding Bacillus thuringiensisδ‐endotoxins

Oddou

Hartmann

Geiser

1991

European Journal of Biochemistry

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“…After a further 48 hours the medium was removed, the cell sheets being washed 4 times with fresh, unlabelled medium, and then treated with a solution (2-0 ml) of either 1% (w/v) Difco 1: 250 trypsin (Difco Laboratories Inc., Detroit, USA) or crystalline trypsin (Sigma 2X crystallized 500 jug/ml) in Ca2+ and Mg2+ free, Tyrode solution supplemented with 1% (w/v) sodium citrate (Rinaldini, 1959). After 10 minutes at 37°C an equal volume of 15% (v/v) serum in Waymouth's medium was added to each bottle to inhibit the trypsin and the cells were dislodged by gentle agitation.…”

mentioning

confidence: 99%

Uptake of 63Ni2+ from its Complexes with Proteins and other Ligands by Mouse Dermal Fibroblasts in vitro

Webb¹,

Weinzierl²

1972

Br J Cancer

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Summary.-A study has been made of the uptake of 63Ni2+ from its complexes with serum proteins and with the small molecules from a sterile autolysate of rat muscle by cells of the C57S/IP line of mouse dermal fibroblasts in vitro. Nickel from these complexes is incorporated intracellularly, the distribution being independent of the nature of the carrier. The cation is associated with all subcellular fractions, the largest amount being bound by the nuclei and the least by the microsomes. About half of the 63Ni2+ incorporated into the nuclei is located in the nucleoli.IT has been shown that the carcinogenic metals, cobalt, cadmium and nickel dissolve when incubated aseptically in horse serum and in homogenates of rat muscle with the formation of complexes of various biological ligands (Heath, Webb and Caffrey, 1969;Weinzierl and Webb, 1972). After solution in serum the dissolved cations are bound by both large (e.g. protein) and small (diffusible) molecules, whereas in muscle homogenates they are complexed almost entirely (e.g. 90 %) with compounds of low molecular weight. The products obtained on solution of nickel in a muscle homogenate in vitro are similar to those formed when implants of the metal dissolve in whole muscle in vivo, and thus it seems that these small molecular complexes may be the normal cation carriers that are involved in the transport of the cation during carcinogenesis. The cobalt serum complex obtained by dissolution of the metal in horse serum, however, also produces cytological changes in cultures of rat myoblasts similar to those seen in pre-malignant cells in vivo (Heath et al., 1969).To investigate whether carcinogenic metals, when bound by ligands of both high and low molecular weight, are incorporated by mammalian cells, a study has

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“…Cells subjected to washing, centrifugation, or repeated pipetting are known to be adversely affected, perhaps through the loss of membrane or cytoplasmic components (17,21,28). A magnetic stirbar or pipetting are commonly employed in disaggregation of cardiac tissue.…”

Section: Discussionmentioning

confidence: 99%

“…Various enzymes have been used to isolate myocardial cells (9,15,28,30,33). Some workers have suggested the use of enzymes other than trypsin and some note that highly purified enzymes may be advantageous.…”

Section: Discussionmentioning

confidence: 99%

Culture of Spontaneously Contracting Myocardial Cells from Adult Rats

Jacobson

1977

Cell Struct. Funct.

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An improved method for the isolation and quantitative cultivation of embryonic cells

Cited by 136 publications

References 16 publications

Identification and characterization of Heliothis virescens midgut membrane proteins binding Bacillus thuringiensisδ‐endotoxins

Identification and characterization of Heliothis virescens midgut membrane proteins binding Bacillus thuringiensisδ‐endotoxins

Uptake of 63Ni2+ from its Complexes with Proteins and other Ligands by Mouse Dermal Fibroblasts in vitro

Culture of Spontaneously Contracting Myocardial Cells from Adult Rats

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