An improved method for the selective detection of fungi in hospital waters by solid phase cytometry

Vos, M.M. De; Nelis, Hans

doi:10.1016/j.mimet.2006.05.020

Cited by 10 publications

(4 citation statements)

References 27 publications

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“…Previously, fluorescent probes have been evaluated for viability assessment in fungi and yeasts (Brul et al. 1997; De Vos and Nelis 2006; Vanhee et al. 2008).…”

Section: Discussionmentioning

confidence: 99%

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Potential for preventing spread of fungi in air-conditioning systems constructed using copper instead of aluminium

Weaver

Michels

Keevil

2010

Letters in Applied Microbiology

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Aims: As copper has been previously suggested as an antimicrobial surface, we tested the effectiveness of copper as an antifungal surface which could be used in air‐conditioning systems as an alternative to aluminium. Methods and Results: Coupons of copper (C11000) and aluminium were inoculated with fungal isolates (Aspergillus spp., Fusarium spp., Penicillium chrysogenum and Candida albicans) for various time periods. Culture on potato dextrose agar and an in situ viability assay using the fluorochrome FUN‐1 were used to determine whether spores had survived. The results showed increased die off of fungal isolates tested compared to aluminium. In addition, copper also prevented the germination of spores present, thereby reducing the risk of the release of spores. Conclusions: Copper offered an antifungal surface and prevented subsequent germination of spores present. FUN‐1 demonstrated that fungal spores entered into a viable but not culturable (VBNC) state on copper indicating the importance of using such methods when assessing the effect of an antifungal as culture alone may give false results. Significance and impact of study: Copper offers a valuable alternative to aluminium which could be used in air‐conditioning systems in buildings, particularly in hospital environments where patients are more susceptible to fungal infections.

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“…Previously, fluorescent probes have been evaluated for viability assessment in fungi and yeasts (Brul et al. 1997; De Vos and Nelis 2006; Vanhee et al. 2008).…”

Section: Discussionmentioning

confidence: 99%

“…2008). It has been reported that using fluorescent viability assays detected more fungi in dialysis water compared to plate counting, possibly indicating the presence of a viable but not culturable (VBNC) state in fungi (De Vos and Nelis 2006; Vanhee et al. 2008).…”

Section: Discussionmentioning

confidence: 99%

Potential for preventing spread of fungi in air-conditioning systems constructed using copper instead of aluminium

Weaver

Michels

Keevil

2010

Letters in Applied Microbiology

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“…The selectivity of an existing commercial SPC (solid phase cytometry) procedure for fungi, proposed by one manufacturer has to be substantially modified to reduce the interference from bacteria and to optimize the labeling of fungi. 7 Also, FTIR (Fourier transform infrared spectroscopy) coupled with chemometric methods, is becoming an important tool in micro-organisms differentiation. 8 These methodologies are expensive or time consuming.…”

Section: Introductionmentioning

confidence: 99%

Discrimination of motile bacteria from filamentous fungi using dynamic speckle

et al. 2012

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Abstract. We present a dynamic laser speckle method to easily discriminate filamentous fungi from motile bacteria in soft surfaces, such as agar plate. The method allows the detection and discrimination between fungi and bacteria faster than with conventional techniques. The new procedure could be straightforwardly extended to different micro-organisms, as well as applied to biological and biomedical research, infected tissues analysis, and hospital water and wastewaters studies.

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“…Filamentous yeast occur widely in environmental samples including drinking water (De Vos and Nelis, ; Pereira et al , ) and activated sludge (Zheng et al , ). The traditional enumeration method for yeast cells, i.e.…”

Section: Introductionmentioning

confidence: 99%

Improvement of FISH–FCM enumeration performance in filamentous yeast species in activated sludge by snailase partial digestion

Cui

Zhang

Han

et al. 2012

Yeast

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This paper developed a novel strategy to improve the fluorescence in situ hybridizationflow cytometry (FISH-FCM) enumeration performance in filamentous yeast species in activated sludge by snailase partial digestion to fully disaggregate filamentous yeast chains into single cells. A 2 h 2% snailase partial digestion liberated more rod-shaped yeast single cells from intertwined filamentous yeast samples than did sonication disaggregation, based on an optical microscopic observation and the forward-light-scatter frequency histogram of FCM analysis. However, adding snailase resulted in a fluorescencequenching phenomenon of the hybridized filamentous yeast cells, which was minimized by lowering the snailase concentration. An approximately 3 h 0.5% snailase partial digestion conducted between sonication and hybridization significantly improved the FISH-FCM enumeration performance for filamentous yeast species by 37%. The results presented here will facilitate the rapid detection, identification and exact enumeration of specific filamentous fungal species in environmental samples.

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An improved method for the selective detection of fungi in hospital waters by solid phase cytometry

Cited by 10 publications

References 27 publications

Potential for preventing spread of fungi in air-conditioning systems constructed using copper instead of aluminium

Potential for preventing spread of fungi in air-conditioning systems constructed using copper instead of aluminium

Discrimination of motile bacteria from filamentous fungi using dynamic speckle

Improvement of FISH–FCM enumeration performance in filamentous yeast species in activated sludge by snailase partial digestion

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