An improved method of 90% pancreatectomy using a low dose of streptozotocin at the pancreaticoduodenal artery results in a rapid diabetic stage in dogs

Abstract

An improved method of inducing diabetes in dogs was developed. This method included 90% pancreatectomy, 2 mg/kg streptozotocin (STZ) perfused into pancreaticoduodenal artery, and the fixation suture of the duodenum to the costo-abdominal wall. Vasopressin injection administered to the animals before surgery reduced bleeding. All dogs used in this procedure survived and became diabetic. One month after the procedure the pancreatic islets were reduced in volume and the number compared with pancreas tissue obtained during the surgery. Acinar tissue remained with a normal histology, and exocrine function maintained the physiological parameters, except for a soft faecal consistency. We conclude that this procedure is effective in inducing experimental diabetes in dogs.

Induction of Type I Diabetes Mellitus in Beagle Dogs Using Alloxan and Streptozotocin

“…Although selective breeding can be used to produce canine subjects with naturally occurring type I diabetes, this is time‐consuming, as dogs tend to develop the disease later in life (Olivares et al., 2017; Qadri et al., 2015) with a median diagnosis age of 9 years (Catchpole, Ristic, Fleeman, & Davison, 2005). Pancreatectomies, removal of β‐cells from the pancreas, injection of a low dose of STZ directly into the pancreaticoduodenal artery, and administration of combined ALX/STZ doses directly into the suprarenal artery are other methods that can be used to induce diabetes in dogs; however, these methods are performed surgically and are therefore more technically difficult (Olivares et al., 2017; Pimentel Morales et al., 2005; Salis et al., 2001). Alternatively, chemical induction of diabetes through IV administration is relatively simple to carry out in a laboratory setting.…”