An improved method of preparing wet mounts for photomicrographs of microorganisms

Pfennig, Norbet; Wagener, Stefan

doi:10.1016/0167-7012(86)90043-6

Cited by 170 publications

(77 citation statements)

References 2 publications

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“…The morphology of cells was studied with a Reichert Zetopan anoptral microscope. Phase-contrast micrographs of bacteria were taken using agar-coated slides (Pfennig & Wagner, 1986). To prepare whole-cell specimens for electron microscopy, cells were sedimented by centrifugation, resuspended in tap water and negatively stained with 1 % (w\v) phosphotungstate pH 7.…”

Section: Methodsmentioning

confidence: 99%

Thermanaerovibrio velox sp. nov., a new anaerobic, thermophilic, organotrophic bacterium that reduces elemental sulfur, and emended description of the genus Thermanaerovibrio.

Заварзина¹,

Zhilina²,

Tourova³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

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Section: Methodsmentioning

confidence: 99%

Thermanaerovibrio velox sp. nov., a new anaerobic, thermophilic, organotrophic bacterium that reduces elemental sulfur, and emended description of the genus Thermanaerovibrio.

Заварзина¹,

Zhilina²,

Tourova³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

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“…For dispensation of anoxic medium into (serum) bottles or culture tubes, the medium was prepared in a 3-liter Erlenmeyer flask equipped with a butyl rubber stopper and a device similar to that described by Widdel and Bak Morphology. Phase-contrast microscopy (Labophot; Nikon, Tokyo, Japan) was used for routine examinations and for photomicrography using the agar slide method (29). Platinum-palladium-shadowed cells were examined in an electron microscope (100 C X JEOL, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

Desulfobacter vibrioformis sp. nov., a Sulfate Reducer from a Water-Oil Separation System

Lien

Beeder

1997

International Journal of Systematic Bacteriology

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A mesophilic, gram-negative, vibrio-shaped, marine, acetate-oxidizing sulfate reducer (strain B54) was isolated from a water-oil separation system on a North Sea oil platform. The optimum conditions for growth were 33"C, pH 6.8 to 7.0, and concentrations of NaCl and MgCl, * 6H,O of at least 1 and 0.3%, respectively. Of various organic acids tested, only acetate was used as an electron and carbon source. The presence of 2-oxog1utarate:dye oxidoreductase suggests acetate oxidation via an operative citric acid cycle. Even though growth of most Desulfobacter strains (including strain B54) did not occur on hydrogen, hydrogenase was detected at low activity. The growth yields were 4.6, 13.1, and 9.6 g of (dry weight) cells per mol of acetate oxidized with sulfate, sulfite, and thiosulfate, respectively, as electron acceptors. Strain B54 was able to fix dinitrogm. Desulforubidin and cytochromes of the c and b types were present. The G+C content of the DNA was 47 mol%. Strain B54 is most closely related to Desulfobacter lutus, with a 16s rDNA sequence similarity of 98.1%. The DNA-DNA relatedness between them was 40.5%. On the basis of differences in genotypic, phenotypic, and immunological characteristics, we propose that strain B54 is a member of a new species, D. vibrioformis. It can be easily identified and distinguished from other Desulfobacter species by its large, vibrioshaped cells.In 1977, Widdel and Pfennig (44) isolated the first authenticated sulfate-reducing bacterium, Desulfotomaculum acetoxiduns, which is able to oxidize acetate completely to carbon dioxide. Later, several new acetate-oxidizing sulfate reducers, both mesophilic and thermophilic, were described (4,28,43,45). Most of these completely oxidizing sulfate reducers are nutritionally versatile, using the oxidative CO dehydrogenase pathway for acetate oxidation (36). The genus Desulfobacter, however, comprises nutritionally specialized sulfate reducers with acetate as their characteristic substrate, which they oxidize via a modified citric acid cycle (16, 25). The genus was established in 1981 by the description of the type species Desulfobacterpostgatei (43). New Desulfobacter species were isolated in 1987, and the genus description was then emended to include H,-utilizing, curved, and vibrio-shaped cells (40). Only a few Desulfobacter species, D. postgatei, D. hydrogenophilus, D. latus, and D. cuwatus, have been validly described (40). Although other members of the Desulfobacter genus have been recently detected, e.g., by oligonucleotide probes, in various locations like bioreactors (33), oil field environments (7, 39), and photosynthetic biofilms (32), none of them has been isolated and characterized.In this paper, we describe the general characteristics and phylogenetic relations of a marine, vibrio-shaped, sulfate-reducing bacterium belonging to the genus Desulfobacter. The strain which we describe B54T [T = type strain]) was enriched and isolated with acetate and sulfate in a medium inoculated with water from the oil-water separation system o...

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“…Cells of strain TBC1 T were immobilized on agar-coated slides (Pfennig & Wagener, 1986). Cells showed a short-rod morphology with round edges; width was around 0.4-0.6 µm and length was around 2-4 µm (Fig.…”

mentioning

confidence: 99%

Lentimicrobium saccharophilum gen. nov., sp. nov., a strictly anaerobic bacterium representing a new family in the phylum Bacteroidetes, and proposal of Lentimicrobiaceae fam. nov.

Sun

Toyonaga

Ohashi

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

187

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Lentimicrobium saccharophilum gen. nov., sp. nov., a strictly anaerobic bacterium representing a new family in the phylum Bacteroidetes, and proposal of Lentimicrobiaceae fam. nov. A novel, strictly anaerobic, short rod-shaped bacterium, designated strain TBC1 T , was isolated from methanogenic granular sludge in a full-scale mesophilic upflow anaerobic sludge blanket reactor treating high-strength starch-based organic wastewater. Cells of this strain were 2-4 µm long and 0.4-0.6 µm wide. They were non-motile and Gram-stain-negative. The optimum growth temperature was 30-37 C, with a range of 20-40 C. The optimum pH for growth was around pH 7.0, while growth occurred in a range of pH 6.5-9.0. Strain TBC1 T grew chemoorganotrophically on a narrow range of carbohydrates under anaerobic conditions. Yeast extract was required for its growth. The major fermentative end products from glucose, supplemented with yeast extract, were acetate, malate, propionate, formate and hydrogen. Doubling time under optimal growth conditions was estimated to be 1 day. The DNA G+C content of strain TBC1 T was 49.2 mol% as determined by HPLC. Major cellular fatty acids were C 16 : 0 , C 18 : 0 , C 16 : 1 !9c and C 18 : 1 !9c. Based on its 16S rRNA gene sequence, strain TBC1 T was shown to represent a distinct lineage at the family level in the phylum Bacteroidetes. Among previously described species of this phylum, Mucilaginibacter boryungensis BDR-9 T (Sphingobacteriaceae) displayed the highest sequence similarity (85.9 %) with strain TBC1 T . Phylogenomic analyses using 38-83 single copy marker genes also supported the novelty of strain TBC1 T at the family level. Based on its characteristics, strain TBC1 T (=JCM 30898 T =DSM 100618 T ) is considered to be the type strain of a novel species of a new genus, Lentimicrobium saccharophilum gen. nov., sp. nov. A new family, Lentimicrobiaceae fam. nov., is also proposed encompassing the strain and related environmental 16S rRNA gene clone sequences.Abbreviations: AAI, amino acid identity; UASB, upflow anaerobic sludge blanket; CTD, C-terminal domain.The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain TBC1 T is LC049960.A supplementary figure is available with the online Supplementary Material.

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An improved method of preparing wet mounts for photomicrographs of microorganisms

Cited by 170 publications

References 2 publications

Thermanaerovibrio velox sp. nov., a new anaerobic, thermophilic, organotrophic bacterium that reduces elemental sulfur, and emended description of the genus Thermanaerovibrio.

Thermanaerovibrio velox sp. nov., a new anaerobic, thermophilic, organotrophic bacterium that reduces elemental sulfur, and emended description of the genus Thermanaerovibrio.

Desulfobacter vibrioformis sp. nov., a Sulfate Reducer from a Water-Oil Separation System

Lentimicrobium saccharophilum gen. nov., sp. nov., a strictly anaerobic bacterium representing a new family in the phylum Bacteroidetes, and proposal of Lentimicrobiaceae fam. nov.

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