An improved method of preparing density gradient tubes

Tung, L. H.; Taylor, W. C.

doi:10.1002/pol.1956.120219715

Cited by 54 publications

(10 citation statements)

References 4 publications

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“…Fraction I obtained after Sepharose 6B chromatography of mucosal cell supernatants was concentrated by ultrafiltration to a protein concentration of approximately 10 mg/ml. 0.5 ml of the concentrate was applied to a continuous glycerol gradient formed by mixing 60% and 20% glycerol solutions according to the method of Tung & Taylor (1956). The sample was subjected to centrifugation on the glycerol gradient as described by Eichholz & Crane (1965) for 10 min at 6.3 x 103 g. The "Fe content was counted and the alkaline phosphatase, intestinal disaccharidase and RNA concentrations were determined by standard methods (Bessey et a!, 1946; Plotkin & Isselbacher, 1964; Munro & Fleck, 1966) on 250pl fractions withdrawn from the bottom of the tube after centrifugation.…”

Section: Glycerol Density Gradient Centrgugationmentioning

confidence: 99%

Iron Absorption in the Rat: The Search for Possible Intestinal Mucosal Carriers

1976

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The biological relevance of four iron-containing fractions previously detected in rat intestinal mucosal cells has been studied. The distribution of iron in these fractions obtained by chromatography on Sepharose 6B has been examined after in vivo and in vitro incubation of mucosal cells with 59Ce. In addition, the effects of phenobarbitone, cycloheximide, iron-deficiency and iron-loading on the uptake and distrubution of iron within the four mucosal cell fractions was studied. The iron in fraction I was mostly bound to intracellular membrane particles. Fraction II was shown to be ferritin. Fraction III contained some transferrin and also a protein of molecular weight similar to transferrin but which was not precipitable by antitransferrin antiserum. Quantited with the results of 'chaser' experiments suggested that, in addition to ferritin, at least two of the fractions (I and III) were involved in the process of iron absorption by the mucosal cell.

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Section: Glycerol Density Gradient Centrgugationmentioning

confidence: 99%

Iron Absorption in the Rat: The Search for Possible Intestinal Mucosal Carriers

1976

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“…The column had a density gradient from 880 kg m À3 to 930 kg m À3 and was prepared using distilled water and isopropyl alcohol according to the method described by Tung and Taylor. 17 The samples were allowed to seek their equilibrium position in the column for approximately 6 h. The shrinkage behavior of the tapes was investigated by immersion in a warmed oil bath for 120 sec at 1008C. Subsequently, the shrinkage was determined as a percentage of the original sample length of 300 mm.…”

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On the overdrawing of melt‐spun isotactic polypropylene tapes

Schimanski

Loos

Peijs

et al. 2006

J of Applied Polymer Sci

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The morphology and mechanical properties of melt-spun and post-drawn isotactic polypropylene (iPP) tapes have been studied in order to examine their dependence on the post-draw ratio applied. Special attention is focused on the characterization of the so-called overdrawing behavior of the tapes; at a certain draw ratio, a change of the optical appearance of the tape from transparent to opaque is observed. Overdrawing is accompanied with changes in the mechanical properties, surface, and morphology of the tapes. For postdraw ratios without overdrawing, and for highly overdrawn tapes, the variation in the mechanical properties measured is very small, but for slightly overdrawn tapes, a large scattering of mechanical properties has been measured. In the latter case, this behavior is related to the start of internal delamination of the tapes during post drawing. It is shown that strongly overdrawn tapes have excellent mechanical properties, in particular, high specific stiffness and strength.

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“…[22][23][24] Theoretical maximum density (TMD) of TATB is 1.937 g/cc. 25 Density distributions of dry and wet aminated TATB in Figure 3 were almost identical with the mean density of wet aminated being 1.9173 g/cc or about 98.98% TMD and dry aminated mean density of 1.9181 g/cc or about 99.02% TMD.…”

Section: Density Distributions Of Tatbsmentioning

confidence: 99%

Comparison of New and Legacy TATBs

Hoffman

Willey

Mitchell

et al. 2008

Journal of Energetic Materials

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Two newly synthesized versions of the insensitive high explosive (IHE) 1,3,5-triamino-2,4,6-trinitrobenzenes (TATBs) were compared to two legacy explosives currently used by the Department of Energy. Except for thermal analysis, small scale safety tests could not distinguish between the different synthetic routes.Morphologies of new TATBs were less faceted and more spherical. The particle size distribution of one new material was similar to legacy TATBs, but the other was very fine. Densities and submicron structure of the new TATBs were also significantly different from the legacy explosives. Pressed pellets of the new explosives were less dense. Recrystallization from sulfolane improved the density and thermal stability of both new TATBs, though the morphology of the recrystallized TATB was nearly hexagonal platelets.

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An improved method of preparing density gradient tubes

Cited by 54 publications

References 4 publications

Iron Absorption in the Rat: The Search for Possible Intestinal Mucosal Carriers

Iron Absorption in the Rat: The Search for Possible Intestinal Mucosal Carriers

On the overdrawing of melt‐spun isotactic polypropylene tapes

Comparison of New and Legacy TATBs

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