An improved MIT assay

Śladowski, Dariusz; Steer, Sarah; Clothier, Richard H.; Balls, Michael

doi:10.1016/0022-1759(93)90088-o

Cited by 355 publications

(191 citation statements)

References 11 publications

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“…Macrophages were treated with the products from 12.5 to 200 μg/mL for 72 h. Cultures with DMSO were included as control treated. The cytotoxicity was determined using the colorimetric assay with 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) (Sigma, St. Louis, MO, USA) solution (15 L at 5 mg/mL in PBS) (Sladowski et al, 1993). The median cytotoxic concentration (CC 50 ) was obtained from lineal doseresponse curves.…”

Section: Cytotoxicity Assaymentioning

confidence: 99%

Antileishmanial activity of leaf extract from Calophyllum rivulare against Leishmania amazonensis

Oubada

García

Bello

et al. 2014

Emir. J. Food Agric

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Calophyllum rivulare is an endemic tree from Cuba that belongs to Clusiaceae family. This plant had revealed it to be a rich source of pyranochromanone acids including compounds with a variety of biological potentialities such as antioxidant, antiulcer, anticancer and anti-Helicobacter pylori activities. Herein, antileishmanial and cytotoxic activities of extracts obtained from C. rivulare leaves (rich in pyranochromanone acids) and amentoflavone were compared. Extracts were prepared by maceration employing ethyl acetate (CR-1) and methanol (CR-2) as solvents and amentoflavone was isolated from both extracts. The CR-2 extract showed the better activity against the parasite, with an IC 50 value of 95.1 ± 5.4 µg/mL and 20.6 ± 1.4 µg/mL against promastigote and amastigote forms, respectively. This extract showed lower cytotoxicity (IC 50 =182.4 ± 2.3 µg/mL) against peritoneal macrophage from BALB/c mice and a selectivity index of 9. Amentoflavone showed an IC50 of 5.9 ± 0.3 µg/mL against amastigote form and a selectivity index of 9. Results suggest that CR-2 extract and amentoflavone could be explored as new antileishmanial alternatives.

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Section: Cytotoxicity Assaymentioning

confidence: 99%

Antileishmanial activity of leaf extract from Calophyllum rivulare against Leishmania amazonensis

Oubada

García

Bello

et al. 2014

Emir. J. Food Agric

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show abstract

“…One day later, MTT assays were performed as previously described (Sladowski et al, 1993). For analysis of the response to oxidative stress, cells were exposed to 200 mM H 2 O 2 (Sigma) for 0.5 ± 6 h as described (Yakes and Van Houten, 1997) and immediately analysed.…”

Section: Mtt Assaymentioning

confidence: 99%

p53-independent apoptotic effects of the hepatitis B virus HBx protein in vivo and in vitro

et al. 1998

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The hepatitis B virus protein HBx is a promiscuous transactivator implicated in both cell growth and death and in the development of hepatocellular carcinoma. We recently reported that HBx can potentiate c-myc-induced liver oncogenesis in a transgenic model where low level expression of HBx induces no pathology. To assess if HBx could a ect the hepatocyte turnover, we investigated the HBx-elicited apoptotic responses in transgenic livers and in primary hepatocyte cultures. Here we show that transgenic expression of HBx is associated with a twofold increase of spontaneous cell death in the mouse liver. The ®nding that apoptosis was enhanced to similar extents in HBx mice carrying homozygous p53 null mutations implied that functionally intact p53 was not required to transduce the death signal. A direct, dosedependent apoptotic function of HBx was demonstrated in transient transfections of liver-derived cell lines. We further show that stable expression of HBx at low, presumably physiological levels in primary hepatocytes, induced cellular susceptibility to diverse apoptotic insults, including growth factor deprivation, treatment with antiFas antibodies or doxorubicine and oxidative stress. HBx expression, but not p53 status profoundly a ected the commitment of cells to die upon apoptotic stimuli. These data strengthen the notion that HBX may contribute to HBV pathogenesis by enhancing apoptotic death in the chronically infected liver.

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“…After incubation for an additional four hours the formazan crystals were dissolved by the addition of 100 μL of DMSO. The optical density was determined using an EMS Reader MF Version 2.4-0, at a wavelength of 560 nm 30 . This test was performed in triplicate.…”

Section: Cytotoxicity Assaymentioning

confidence: 99%

Leishmanicidal activity of Echinaster (Othilia) echinophorus crude extract

Parra

Fidalgo

Martínez

et al. 2010

Rev. Inst. Med. trop. S. Paulo

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SUMMARYIn this study, a methanolic extract from Echinaster (Othilia) echinophorus was evaluated for activity against Leishmania amazonensis. The extract showed activity against the promastigote and amastigote forms with IC 50 values of 62.9 and 37.5 μg.mL -1 respectively. This extract showed a moderate toxicity on macrophages from BALB/c mice. A dose of 100 mg/kg/day was effective when administered during 15 days by intraperitoneal route to BALB/c mice infected experimentally.

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An improved MIT assay

Cited by 355 publications

References 11 publications

Antileishmanial activity of leaf extract from Calophyllum rivulare against Leishmania amazonensis

Antileishmanial activity of leaf extract from Calophyllum rivulare against Leishmania amazonensis

p53-independent apoptotic effects of the hepatitis B virus HBx protein in vivo and in vitro

Leishmanicidal activity of Echinaster (Othilia) echinophorus crude extract

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