An Improved Oil Emulsion Synthesis Method for Large, Porous Zirconia Particles for Packed- or Fluidized-Bed Protein Chromatography

Robichaud, Michael J.; Sathyagal, Arun; Carr, Peter W.; McCormick, Alon V.; Flickinger, Michael C.

doi:10.1080/01496399708000785

Cited by 8 publications

(4 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One series of papers [135,147,[155][156][157][158][159][160][161][162][163] affords a comparison of the type of pore structure, sintering behavior, diffusion behavior, and chromatographic performance of mesoporous zirconia made of the same colloid through two different methods to make spherical aggregates-that using an oil emulsion (cf. [13]) versus that using polymerization method (cf.…”

Section: Effect Of Synthesis Methods On the Pore Structurementioning

confidence: 99%

“…This produces ca. 10 m spheres with pores ranging from 100 to 300 Å. Carr and co-workers [147] used a similar procedure, with a commercially available colloid and modified by rheological control of the aqueous phase and by the introduction of a reactive binder to enhance the stability and monodispersity of the resulting microspheres (however, they tended to make spheres on the order of 50 m in diameter). Shalliker et al [148][149][150][151][152][153][154] used a similar procedure, enhancing the rate and perhaps stability of the gel structure with the addition of urea.…”

Section: Zirconiamentioning

confidence: 96%

“…Carr et al [17,135,147,[155][156][157][158][159][160][161][162][163] took an approach related to Iler's to make monodisperse, micron-scale spherical microparticles out of commercially available colloid through the addition of urea and formaldehyde. Pore structure and particle size variations were investigated as preparation and sintering conditions were manipulated, and the most striking result was that very strong, very porous (up to 50% porous), very large pore (200 Å minimum aperture) monodisperse spherical particles could be made.…”

Section: Zirconiamentioning

confidence: 99%

See 2 more Smart Citations

Part I. Chromatography using ultra-stable metal oxide-based stationary phases for HPLC

Nawrocki

Dunlap

McCormick

et al. 2004

Journal of Chromatography A

316

187

View full text Add to dashboard Cite

The first part of the review contrasts the main drawbacks of silica-based packings such as their relative thermal and chemical instability with excellent stability of metal oxides. The paper concerns mainly ZrO2, TiO2 and Al2O3. Methods of preparation of spherical particles for HPLC are described. Surface chemistry of the oxides is, however, very different from that of silica. Ability of the oxides to ion- and ligand exchange is discussed from a chromatographic point of view.

show abstract

Section: Effect Of Synthesis Methods On the Pore Structurementioning

confidence: 99%

Section: Zirconiamentioning

confidence: 96%

Section: Zirconiamentioning

confidence: 99%

See 1 more Smart Citation

Part I. Chromatography using ultra-stable metal oxide-based stationary phases for HPLC

Nawrocki

Dunlap

McCormick

et al. 2004

Journal of Chromatography A

316

187

View full text Add to dashboard Cite

show abstract

“…The FBOM-zirconia particles were synthesized by the method of Robichaud et al (1997) and were screened with US standard sieves to select particles in the size range 75-103 m. The particles were washed with acid and base and elutriated prior to use as described elsewhere (Griffith et al, 1997). The bed was expanded using ddH 2 O with flow rates up to 75 mL/min.…”

Section: Expanded Bed Rtd and Protein Adsorptionmentioning

confidence: 99%

Expanded and packed bed albumin adsorption on fluoride modified zirconia

Mullick¹,

Griffith²,

Flickinger³

1998

Biotechnol. Bioeng.

Self Cite

View full text Add to dashboard Cite

The expanded bed characteristics of 75–103μm fluoride‐modified zirconia (FmZr) particles synthesized by a fed batch oil emulsion process were investigated. These particles are distinguished from commercially available expanded‐bed adsorbents by virtue of their high density (2.8 g/cc) and the mixed mode protein retention mechanism which allows for the retention of both cationic and anionic proteins. The linear velocity versus bed porosity data agree with the Richardson–Zaki relationship with the terminal velocity in infinite medium of 2858.4 cm/h and a bed expansion index of 5.1. Residence time distribution (RTD) studies and bovine serum albumin (BSA) adsorption studies were performed as a function of the height of the settled bed to the column diameter (H:D) ratio and degree of bed expansion with superficial velocities of 440 to 870 cm/h. The settled bed, a 2× expanded bed, and a 3× expanded bed were studied for the H:D ratios of 1:1, 2:1, and 3:1. The dynamic binding capacity (DBC) at 5% breakthrough was low (2–8 mg BSA/mL settled bed) and was independent of the H:D ratio or the degree of bed expansion. The saturation DBC was 32.3 ± 7.0 mg BSA/mL settled bed. The adsorption–desorption kinetics and intraparticle diffusion for protein adsorption on FmZr (38–75 μm) were investigated by studying the packed bed RTD and BSA adsorption as a function of temperature and flow rate. The data show that the adsorption–desorption kinetics along with intraparticle diffusion significantly influence protein adsorption on FmZr. Low residence times (∼0.8 min) of BSA result in a DBC at 5% breakthrough which is 3.5‐fold lower compared to that at 6‐fold higher protein residence time. At low linear velocity (45 cm/h) the breakthrough curve is nearly symmetrical and becomes asymmetrical and more dispersed at higher linear velocity (270 cm/h) due to the influence of slow adsorption–desorption kinetics and intraparticle diffusion.© 1998 John Wiley & Sons, Inc. Bioeng 60: 333–340, 1998.

show abstract

Expanded bed adsorption of human serum albumin from very densesaccharomyces cerevesiae suspensions on fluoride-modified zirconia

Mullick

Flickinger

1999

Biotechnol. Bioeng.

View full text Add to dashboard Cite

An Improved Oil Emulsion Synthesis Method for Large, Porous Zirconia Particles for Packed- or Fluidized-Bed Protein Chromatography

Cited by 8 publications

References 11 publications

Part I. Chromatography using ultra-stable metal oxide-based stationary phases for HPLC

Part I. Chromatography using ultra-stable metal oxide-based stationary phases for HPLC

Expanded and packed bed albumin adsorption on fluoride modified zirconia

Expanded bed adsorption of human serum albumin from very densesaccharomyces cerevesiae suspensions on fluoride-modified zirconia

Contact Info

Product

Resources

About