An Improved Preparation of Sodium Desoxyribonucleate

Fluorescence spectral evidence that benzo(a)pyrene‐DNA products in mouse skin arise from diol‐epoxides

“…Benzo(u)pyrene, purified by recrystallization from xylene, and DNA (salmon sperm, type III), deproteinized by a detergent salt procedure [6], were purchased from Sigma Chemical Co., St, Louis, MO., U.S.A. m-Chloroperoxybenzoic acid was obtained from BDH Chemicals Ltd., Poole, Dorset, U. K. 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene and 9,10-dihydro-9,10-dihydroxybenzo(u)pyrene were obtained from the hydrocarbon by metabolism [7] ; the corresponding diol-epoxides, 7,8-dihydro-7,%dihydroxy-benz(u)pyrene 9,10-oxide and 9,10-dihydro-9,10-dihydroxybenzo(a)pyrene 7,8-oxide were prepared from the diols by oxidation with m-chloroperoxybenzoic acid in benzene. Benzo(a)pyrene 4,5oxide was also synthesized [SJ .…”

An ultracentrifugation analysis of two hundred fish genomes

“…The samples of fish DNAs investigated here were obtained from either liver or, in the case of small fishes, from whole bodies, using the method of Kay et al (1952).…”

Polycyclic hydrocarbon epoxides: The involvement of 8,9‐dihydro‐8,9‐dihydroxybenz (a) anthracene 10,11‐oxide in reactions with the DNA of benz (a) anthracene‐treated hamster embryo cells

“…8,9-Dihydro-8,9-dihydroxybenz(a)anthracene 10, 1 l-oxide (2.5 mg) or benz(a)anthracene 5,6-oxide (2.5 mg) in ethanol (25 ml) was mixed with a solution of Salmon sperm DNA (50 mg) previously deproteinized by a detergent-salt procedure [9], in Tris buffer pH 7.4, 0.01 M (50 ml) and incubated at 37'C for 4 hr. After incubation, the solution was extracted with solvent and the DNA reisolated as described previously [5].…”