An improved procedure for the preparation and measurement of (Na+ + K+)-ATPase in human erythrocytes

Charalambous, Bambos M.; Ma, Minghong

doi:10.1016/0005-2736(82)90215-2

Cited by 9 publications

(3 citation statements)

References 30 publications

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“…This was similar to pre vious observations by Charalambous and Mir [16]. Though collection of specimens in this study was made at two locations, geographical difference of sample process ing in different laboratories was unlikely to occur when all specimens were handled by the same persons and treated in a similar fashion.…”

Section: Discussionsupporting

confidence: 90%

Hypokalemia, High Erythrocyte Na<sup>+</sup> and Low Erythrocyte NaK-ATPase in Relatives of Patients Dying from Sudden Unexplained Death Syndrome in North-East Thailand and in Survivors from Near-Fatal Attacks

Tosukhowong

Chotigasatit²,

Tungsanga³

et al. 1996

Am J Nephrol

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The sudden unexplained death syndrome (SUDS) is a sudden death of unknown cause in healthy South-East Asians. In Thailand, it is common in the North-East region. We previously reported high incidences of low erythrocyte sodium and potassium-activated adenosine triphosphatase (NaK-ATPase) activity and of high erythrocyte sodium in North-East Thais and speculated that this metabolic defect might be associated with the high incidence of SUDS in that region. In this communication, we studied plasma sodium and potassium, erythrocyte sodium and potassium, activities of erythrocyte NaK-ATPase, ouabain-insensitive ATPase and total ATPase in healthy Thai blood donors from Central Thailand (group 1), healthy North-East city dwellers (group 2), relatives of SUDS victims (group 3) and survivors from SUDS-like attacks (group 4). Compared with groups 1 and 2, group 3 and 4 subjects had significantly lower plasma potassium (p < 0.0001), higher erythrocyte sodium (p < 0.0001), lower activities of erythrocyte NaK-ATPase (p < 0.0001) and of erythrocyte total ATPase (p < 0.0001). In addition, group 4 subjects had lower plasma potassium, higher erythrocyte sodium and lower activity of total ATPase than those of group 3. The findings suggest that the pathogenesis of SUDS could be related to hypokalemia and a membrane sodium/potassium pump defect.

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Section: Discussionsupporting

confidence: 90%

Hypokalemia, High Erythrocyte Na<sup>+</sup> and Low Erythrocyte NaK-ATPase in Relatives of Patients Dying from Sudden Unexplained Death Syndrome in North-East Thailand and in Survivors from Near-Fatal Attacks

Tosukhowong

Chotigasatit²,

Tungsanga³

et al. 1996

Am J Nephrol

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“…Erythrocyte vesicles were prepared by the method of Charalambous and Mir (18); this method yields high activities of total ATPase and sodium potassium-ATPase with a coefficient of variation of <8%. Protein concentration was determined by the method of Bradford (19) and ATPase activity by measurement of phosphate release (20).…”

Section: Erythrocyte Cation Transportmentioning

confidence: 99%

Inhibitin: a specific inhibitor of sodium/sodium exchange in erythrocytes.

Morgan¹,

Brown²,

Spurlock³

et al. 1986

J. Clin. Invest.

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An inhibitor of ouabain-insensitive sodium/sodium exchange in erythrocytes has been isolated from leukemic promyelocytes. To explore the specific effects of this inhibitor, named inhibitin, sodium transport experiments were carried out in human erythrocytes. Inhibitin reduced ouabain-insensitive bidirectional sodium transport. It did not change net sodium fluxes, had no significant effect on rubidium influx, and did not inhibit sodiumpotassium-ATPase activity. The inhibitory effect of inhibitin was studied on sodium/sodium exchange and on sodium/lithium countertransport in 140 mM sodium and in sodium-free media. In the presence of sodium, inhibitin reduced sodium and lithium efflux to that observed in sodium-free medium. Inhibitin showed no reduction in sodium or lithium efflux when sodium was replaced by choline chloride or Mg2e. When inhibitin was combined with one or more of the other transport inhibitors (i.e., ouabain, furosemide, or bumetanide and amiloride), its inhibitable component remained distinct and it did not overlap with that of the other inhibitors. These studies show that inhibitin is a specific inhibitor of carrier-mediated sodium/sodium exchange and sodium/lithium countertransport processes in human erythrocytes.

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“…Для человека, мембраны (тени) эритроцитов -это более универсальный объект исследования активности фермента, чем мышечная ткань, поскольку при этом не требуется взятия биопсии. О необходимости стандартизации методов, применяемых для определения активности Na + /K + -АТФазы в эритроцитах, свидетельствуют следующие ав-торы [9,15,16]. Установлено, что в мембранных препаратах эритроцитов активность Na + /K + -АТФазы, по данным различных исследований, колеблет-ся в широких пределах, например, для эритроцитов человека активность фермента варьирует от 0,05 до 0,35 мкмоль/ч на мг белка [9,15,16] 1 этап -выделение теней эритроцитов и их хранение.…”

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Сравнительный Анализ Методов Исследования Активности Mg2+-Зависимой Na+/K+-Активируемой Атфазы В Тенях Эритроцитов

Petrova¹

2017

ВМНО

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An improved procedure for the preparation and measurement of (Na+ + K+)-ATPase in human erythrocytes

Cited by 9 publications

References 30 publications

Hypokalemia, High Erythrocyte Na<sup>+</sup> and Low Erythrocyte NaK-ATPase in Relatives of Patients Dying from Sudden Unexplained Death Syndrome in North-East Thailand and in Survivors from Near-Fatal Attacks

Hypokalemia, High Erythrocyte Na<sup>+</sup> and Low Erythrocyte NaK-ATPase in Relatives of Patients Dying from Sudden Unexplained Death Syndrome in North-East Thailand and in Survivors from Near-Fatal Attacks

Inhibitin: a specific inhibitor of sodium/sodium exchange in erythrocytes.

Сравнительный Анализ Методов Исследования Активности Mg2+-Зависимой Na+/K+-Активируемой Атфазы В Тенях Эритроцитов

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