An improved rapid method for DNA recovery from cotton swabs

Gray, Alexander; Kuffel, Agnieszka; Daéid, Niamh Nic

doi:10.1016/j.fsigen.2023.102848

Cited by 8 publications

(3 citation statements)

References 19 publications

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“…It is known that swabs are useful for collecting DNA and/or bacteria from the lesion area, but poor at releasing the DNA on extraction procedure. Moreover, DNA extraction methods can affect the yield of DNA amount (Adamowicz et al 2014, Gray et al 2023. Therefore, false negative PCR results due to the extraction method might have caused discrepancies in detection rates of the bacteria.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the presence of Chlamydia spp., Mycoplasma spp. and Moraxella ovis in infectious keratoconjunctivitis cases in sheep and goats in Siirt province and evaluation of clinical findings

Gulaydin,

Akgul

et al. 2024

Polish Journal of Veterinary Sciences

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Infectious keratoconjunctivitis is an infectious disease that negatively affects animal welfare causing systemic or local clinical signs in small ruminants and causes significant economic losses in herds. It is important to determine the etiologic agent causing the infection in the development of the protection and control strategies against the disease. The aim of this study was to determine the presence of infectious keratoconjunctivitis cases in small ruminants raised in Siirt province in Türkiye. Infectious keratoconjunctivitis was graded according to the symptoms determined by clinical examination. The presence of Chlamydia spp., Mycoplasma spp. and Moraxella ovis was investigated by PCR in swab samples obtained from the animals with keratoconjunctivitis. Infectious keratoconjunctivitis was detected in 263 (19.86%) of 1324 animals examined in the study. Of the animals with infectious keratoconjunctivitis, 163 (61.97%) were sheep and 100 (38.02%) were goats. The detection rate of infectious keratoconjunctivitis was higher in sheep than goats. In 56 (21.29%), 109 (41.44%), 67 (25.47%), and 31 (11.78%) of the cases, findings of stage 1, 2, 3, and 4 infectious keratoconjunctivitis were detected, respectively. Of the eye swab samples taken from 263 animals with infectious keratoconjunctivitis, 5 (1.90%) were positive for Mycoplasma spp. and 6 (2.28%) were positive for M. ovis. It was determined that the distribution of the bacterial agents varied according to the stage of infectious keratoconjunctivitis. No statistically significant correlation was found in the distribution of bacterial agents among identified samples according to species, sex, age, and infectious keratoconjunctivitis stage of the animals. It was thought that the data obtained in the study would contribute to the studies for protection and control by determining the incidence and aetiology of infectious keratoconjunctivitis cases observed in small ruminants.

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Section: Discussionmentioning

confidence: 99%

Investigation of the presence of Chlamydia spp., Mycoplasma spp. and Moraxella ovis in infectious keratoconjunctivitis cases in sheep and goats in Siirt province and evaluation of clinical findings

Gulaydin,

Akgul

et al. 2024

Polish Journal of Veterinary Sciences

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“…Swabs in this experiment were extracted using our in-house extraction protocol as described by Gray et al [ 52 ] as preliminary experiments showed this method maximised DNA recovery over the available commercial methods. Briefly, swabs were extracted using a polyvinylpyrrolidone (PVP)/tween 20 solution containing proteinase K followed by a heating step to kill proteinase K activity and then direct qPCR of the extract.…”

Section: Methodsmentioning

confidence: 99%

A synthetic fingerprint solution and its importance in DNA transfer, persistence and recovery studies

Arsenault

Daéid

Gray

2023

Forensic Science International: Synergy

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“…BSA gave the best results but cannot be used in combination with prot K as it destroys the BSA. Therefore, PVP was used instead of BSA, ending up with the following optimal buffer composition: 1% PVP, 1% Tween 20, and 20 mM Tris HCl (with 20 µg/mL prot K to remove proteins that are bound to the DNA preventing access for primers and polymerase) [33].…”

Section: Comparison Of Swab Efficiencymentioning

confidence: 99%

What Are the Limitations and Challenges of Swab-Based DNA Sampling?

Bruijns

2024

Forensic Sciences

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Selecting the optimal sampling method is an essential component of the DNA analysis process. Errors or omissions in targeting and gathering relevant samples can significantly reduce the likelihood of obtaining a valuable DNA profile, affecting the profile’s quality and evidential value and ultimately hindering its ability to support the justice system. While DNA typing techniques have become significantly more sensitive in recent years, there is an ongoing need for further advancements in the recovery of DNA from crime scenes. It is essential to improve the accuracy and reliability of forensic investigations, particularly in cases where only tiny amounts of DNA are present, such as touch DNA samples or degraded forensic evidence. Parameters, including swab material, type of substrate, and swabbing protocol, that influence the efficiency of a swab are discussed in this review. This is followed by a literature review of studies that have compared swab types and/or other sampling conditions. While swabs are the most-used collection tools at a crime scene, alternatives are available. These alternatives are reviewed, including their advantages and disadvantages. A critical discussion and conclusions make clear that, unfortunately, neither swabs nor their alternatives are highly effective in recovering DNA from a substrate.

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An improved rapid method for DNA recovery from cotton swabs

Cited by 8 publications

References 19 publications

Investigation of the presence of Chlamydia spp., Mycoplasma spp. and Moraxella ovis in infectious keratoconjunctivitis cases in sheep and goats in Siirt province and evaluation of clinical findings

Investigation of the presence of Chlamydia spp., Mycoplasma spp. and Moraxella ovis in infectious keratoconjunctivitis cases in sheep and goats in Siirt province and evaluation of clinical findings

A synthetic fingerprint solution and its importance in DNA transfer, persistence and recovery studies

What Are the Limitations and Challenges of Swab-Based DNA Sampling?

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