An Improved Technique for Quantitative Detection of Fecal Coliforms and Pseudomonas Aeruginosa on Surfaces and in Air

Petersen, Norman J.; Favero, Martin S.; Bond, Walter W.; Carson, Loretta A.; Marshall, James H.; Collins, Dalis E

doi:10.1007/978-3-642-49267-9_15

Cited by 3 publications

(3 citation statements)

References 7 publications

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“…containing a t raised convex nutrient bed of Trypticase soy agar (BBL) with 0.07% lecithin and 0.5% polysorbate 80 neutralizers to inactivate residual disinfectants from the surface. The bacteriological methods used have been described elsewhere (13,14). Briefly, after incubation for 16 h at 37°C, colonies on the plates were transferred by direct contact onto sterile rayon velvet cloth (purchased locally), from which similar plates containing FC medium (Bacto-mFC agar; Difco Laboratories, Detroit, Mich.) were inoculated.…”

mentioning

confidence: 99%

Fecal coliforms on environmental surfaces in two day care centers

Weniger¹,

Ruttenber²,

Goodman³

et al. 1983

Appl Environ Microbiol

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A survey of environmental surfaces in two Atlanta area day care centers was conducted to determine the prevalence of fecal coliform bacteria, considered a marker for the presence of fecal contamination which might contain pathogenic parasites, bacteria, or viruses. Fecal coliforms were found in 17 (4.3%) of 398 representative samples of building surfaces, furniture, and other objects. These surfaces may be involved in the chain of transmission of enteric diseases among children. Therefore, disinfection of inanimate objects, in addition to good handwashing, may be important in controlling the spread of enteric diseases in day care centers.

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mentioning

confidence: 99%

Fecal coliforms on environmental surfaces in two day care centers

Weniger¹,

Ruttenber²,

Goodman³

et al. 1983

Appl Environ Microbiol

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show abstract

“…Moreover, such techniques typically require the use of physical or chemical agents (11,16,17,18,20,23,25) either to inhibit substantial fractions of the total microbial flora or to enhance the growth or development of distin-guishing characteristics that enable rapid detection of specific organisms. Whether the restrictions imposed by the use of selective media, on which injured or stressed populations may be unable to recover, further compounds the problems of interpreting results obtained in in vitro environments has been questioned by Hoadley and Cheng (16).…”

Section: Discussionmentioning

confidence: 99%

“…The asparagine-MPN system has proven effective in microbiological surveys to detect or enumerate P. aeruginosa in a variety of hospital environments (10,21,22,23) despite the limitations of sample size and procedural difficulties inherent in MPN systems. mF techniques which rely on development of typical colony characteristics may be subject to considerable variation, dependent to some extent on sample sources and differences in the physiological condition of test strains or on the quality of assay media and procedures.…”

Section: Discussionmentioning

confidence: 99%

Factors influencing detection and enumeration of Pseudomonas aeruginosa by most-probable-number and membrane filtration techniques.

1975

Applied Microbiology

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Most-probable-number (MPN) and membrane filtration (mF) techniques were evaluated with respect to selectivity, sensitivity, and efficiency in recovering Pseudomonas aeruginosa strains in hospital fluids and extramural water environments. Known numbers of cells of a naturally occurring strain of P. aeruginosa maintained in distilled water or cells subcultured on Standard Methods agar were added to test samples containing various types and levels of background microbial contaminants. Environmental samples containing unknown numbers ofP. aeruginosa strains also were tested. Asparagine and acetamide broths were employed as presumptive media in MPN tests, and mPA and Pseudosel agars were used in mF assays. Statistical analyses of data showed the superiority and comparability of the asparagine-MPN and mPA-mF systems. Greater precision and accuracy were consistently obtained in either assay technique by the use of naturally occurring cells as test organisms. The type of filter and nature of diluents employed, as well as pH of assay media, were found to greatly influence both recovery and development of characteristic colonial morphology in the mPA-mF system.

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Factors influencing detection and enumeration of Pseudomonas aeruginosa by most-probable-number and membrane filtration techniques

1975

Appl Microbiol

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Most-probable-number (MPN) and membrane filtration (mF) techniques were evaluated with respect to selectivity, sensitivity, and efficiency in recovering Pseudomonas aeruginosa strains in hospital fluids and extramural water environments. Known numbers of cells of a naturally occurring strain of P. aeruginosa maintained in distilled water or cells subcultured on Standard Methods agar were added to test samples containing various types and levels of background microbial contamiants. Environmental samples containing unknown numbers of P. aeruginosa strains also were tested. Asparagine and acetamide broths were employed as presumptive media in MPN tests, and mPA and Pseudosel agars were used in mF assays. Statistical analyses of data showed the superiority and comparability of the asparagine-MPN and mPA-mF systems. Greater precision and accuracy were consistently obtained in either assay technique by the use of naturally occurring cells as test organisms. The type of filter and nature of diluents employed, as well as pH of assay media, were found to greatly influence both recovery and developemnt of characteristic colonial morphology in the mPA-mF system.

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An Improved Technique for Quantitative Detection of Fecal Coliforms and Pseudomonas Aeruginosa on Surfaces and in Air

Cited by 3 publications

References 7 publications

Fecal coliforms on environmental surfaces in two day care centers

Fecal coliforms on environmental surfaces in two day care centers

Factors influencing detection and enumeration of Pseudomonas aeruginosa by most-probable-number and membrane filtration techniques.

Factors influencing detection and enumeration of Pseudomonas aeruginosa by most-probable-number and membrane filtration techniques

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