Since the inception of the vitamin theory, bananas have usually been considered a good source of such essential food factors. Textbooks list the potency of bananas as + to ++ for vitamin A, + to ++ for vitamin B (Bl), ++ for vitamin C, and ++ for vitamin G (B2). One + indicates that the food contains the vitamin, while ++ indicates that the food is a good source of the vitamin. The vitamin D potency of bananas, in common with other fruits and vegetables, is low.The establishment of minimum vitamin requirements for children and adults by Rose (1933), Steibling (1936, and others and the use of International Reference Standards in bioassay techniques have brought about the necessity for a re-analysis of our common foodstuffs f o r their vitamin content. The present study contains the results of vitamin A, B,, and C assays carried out by latest approved techniques upon ripe banana pulp. The bananas used were representative of the banana of commerce in the United States.