Aspergillus is one of the well-known existed saprophytic fungi that can withstand with various environments. Other can be beneficial in food industry, it also can be infectious to human and animals and normally, it attacks those with low immunity level. In order to keep the treatment in track with more accurate analysis, identification of Aspergillus plays an important role. Identification of Aspergillus is solely based on its characteristic and currently, there are two methods used which are microscopic and macroscopic examinations to observe its features. It handled by experienced microscopist and a few confirmations had to be done before presenting out the final result. Therefore, to prevent misidentification, an automated based identification is proposed. In this paper, different supervised classifiers are tested and compared to observe their ability to detect different 162 of Aspergillus images. The features have been extracted by using Principal component analysis (PCA) and several classifiers such as k-nearest neighbour (kNN), Sparse Representation Classifier (SRC), Support Vector Machine (SVM), Improved Fuzzy-Based k Nearest Centroid Neighbor (IFkNCN) and Kernal Sparse Representation Classifier (KSRC) are employed. Based on its accuracy, Aspergillus flavus recorded 80% of accuracy for all the classifiers.