2024
DOI: 10.3390/biomedicines12030505
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An In Silico and In Vitro Assessment of the Neurotoxicity of Mefloquine

Basma M. El Sharazly,
Abrar Ahmed,
Hany M. Elsheikha
et al.

Abstract: Mefloquine (MQ) is a quinoline-based anti-malarial drug used for chemoprophylaxis or as a treatment in combination with artesunate. Although MQ has clear anti-Plasmodium falciparum properties, it can induce neurotoxicity and undesired neuropsychiatric side effects in humans. Hence, this study aimed to characterize the neurotoxicity of MQ using human neuroblastoma SH-SY5Y cells. The effects of MQ on neuronal toxicity and cell viability were investigated over a concentration range of 1–100 µM using 3-(4,5-dimeth… Show more

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“…Cells were washed twice with ice-cold PBS and then their fluorescence was quantified using a Varioskan™ LUX multimode microplate reader (Thermo Fisher Scientific, Waltham, MA, USA) at excitation and emission spectra of 495 nm and 529 nm, respectively. Hydrogen peroxide (0.5 mM) was used as a positive control for ROS, set as 100% fluorescence [46,53]. Three to six replicate assays were performed for all data points, from which an average was calculated.…”
Section: Measurements Of Reactive Oxygen Speciesmentioning
confidence: 99%
“…Cells were washed twice with ice-cold PBS and then their fluorescence was quantified using a Varioskan™ LUX multimode microplate reader (Thermo Fisher Scientific, Waltham, MA, USA) at excitation and emission spectra of 495 nm and 529 nm, respectively. Hydrogen peroxide (0.5 mM) was used as a positive control for ROS, set as 100% fluorescence [46,53]. Three to six replicate assays were performed for all data points, from which an average was calculated.…”
Section: Measurements Of Reactive Oxygen Speciesmentioning
confidence: 99%