An In Situ Hybridization Protocol for Detection and Identification of Planktonic Bacteria

Glöckner, Frank Oliver; Amann, Rudolf; Alfreider, Albin; Pernthaler, Jakob; Psenner, Roland; Trebesius, Karlheinz; Schleifer, Karl‐Heinz

doi:10.1016/s0723-2020(96)80069-5

Cited by 289 publications

(225 citation statements)

References 19 publications

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“…6) indicated a high degree of cell-specific physiological activity, and the EUB probe detected 58-96% of total DAPI-stained bacterial cells. A similar range of values has been reported from oligo-to eutrophic lakes [1,11,26,38,49]. Except for a few samples from the middle part of the reservoir in the July transect, the EUB probe always targeted >65% of total DAPI-stained bacterial cells (Fig.…”

Section: Discussionsupporting

confidence: 84%

“…The BETA lineage has been detected in a broad variety of limnetic systems, but sequences related to BETA were largely absent from clone libraries from open ocean environments. BETA often comprised large proportions or even dominate within freshwater bacterial communities involving bacterioplankton [1,11,26,38], lake snow aggregates [49], and activated sludge [21,47].…”

Section: Discussionmentioning

confidence: 99%

“…A crude analysis of bacterial community structure was carried out by fluorescence in situ hybridization (FISH) with oligonucleotide probes on membrane filters [1,11]. Duplicated reservoir samples were prefixed with alkaline Lugol's solution followed by formalde- hyde (2% w/v, final concentration) for at least 1 h, and decolorized by addition of several drops of a 3% solution of sodium thiosulfate to prevent cell disruption of planktonic algae and of other fragile flagellated plankters.…”

Section: Fluorescence In Situ Hybridization Of Bacteria With Group Spmentioning

confidence: 99%

“…This approach has already distinguished some common and specific features of BCC in different freshwater environments covering a range from oligotrophic lakes [1,26], through oligo-mesotrophic lakes [11,22,49] or eutrophic reservoirs [38], to activated sludge systems [21,47]. Although canyon-shaped reservoirs are unique, highly dynamic systems that involve a range of trophic gradient within the same water body, no specific study on changing phylogenetic bacterial composition on such longitudinally highly heterogeneous systems has been conducted.…”

Section: Introductionmentioning

confidence: 99%

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Changes in the Epilimnetic Bacterial Community Composition, Production, and Protist-Induced Mortality along the Longitudinal Axis of a Highly Eutrophic Reservoir

Šimek¹,

Armengol

Comerma

et al. 2001

Microbial Ecology

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A B S T R A C TWe studied changes in the epilimnetic bacterial community composition (BCC), bacterial biomass and production, and protistan succession and bacterivory along the longitudinal axis of the canyonshaped, highly eutrophic Sau Reservoir (NE Spain) during two sampling campaigns, in April and July 1997. Longitudinal changes in BCC from the river inflow to the dam area of the reservoir were detected by using oligonucleotide probes targeted to the kingdom Bacteria, to the alpha, beta, and gamma subclasses (ALFA, BETA, and GAMA) of the class Proteobacteria, and to the Cytophaga/ Flavobacterium (CF) cluster. In general, the inflow of the organically loaded Ter river, with highly abundant allochthonous bacterial populations, induced a clearly distinguishable longitudinal succession of the structure of the microbial food web. The most dynamic changes in microbial parameters occurred at the plunge point, the mixing area of river water and the reservoir epilimnion. Changes within members of BETA and CF were the most important in determining changes in BCC, bacterial abundance and biomass. Much less relevant changes occurred within the less abundant ALFA and GAMA bacteria. From the plunge point downstream, we described a significant shift in BCC in the form of decreased proportions of BETA and CF. This shift spatially coincided with the highest values of heterotrophic nanoflagellate bacterivory (roughly doubled the bacterial production). CF numerically dominated throughout the reservoir without any marked longitudinal changes in their mean cell volume. In contrast, very large cells affiliated to BETA clearly dominated in the allochthonous bacterial biomass brought by the river. BETA showed a marked downstream trend of decreasing mean cell volume. We conclude that the observed BCC shift and the longitudinal shift in food web structure (bacteria-heterotrophic nanoflagellates-ciliates) resulted from highly complex interactions brought about by several major factors: varying hydrology, the high localized allochthonous input of organic matter brought by the river, downstream changing substrate availability, and selective protistan bacterivory.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Fluorescence In Situ Hybridization Of Bacteria With Group Spmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Changes in the Epilimnetic Bacterial Community Composition, Production, and Protist-Induced Mortality along the Longitudinal Axis of a Highly Eutrophic Reservoir

Šimek¹,

Armengol

Comerma

et al. 2001

Microbial Ecology

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“…Approximately 500 ng of Sr was evaporated onto a Re filament, and Sr isotopes of the samples, as well as Sr standard SRM 987 and modern seawater, were measured using a multidynamic method on a Finnigan MAT 262 multicollector thermal ionization mass spectrometer. A total of 100 ratios were measured for each analysis, and interference from 87 (Glockner et al, 1996). FISH was done with domain-and group-specific oligonucleotide DNA probes (IDT, Integrated DNA Technologies) labeled with Cyanine 3 (Glockner et al, 1996).…”

Section: Samples and Methodsmentioning

confidence: 99%

Isotopic evidence of enhanced carbonate dissolution at a coal mine drainage site in Allegheny County, Pennsylvania, USA

Sharma

Sack

Adams

et al. 2013

Applied Geochemistry

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Editorial handling by A. Kolker a b s t r a c t Stable isotopes were used to determine the sources and fate of dissolved inorganic C (DIC) in the circumneutral pH drainage from an abandoned bituminous coal mine in western Pennsylvania. The C isotope signatures of DIC (d 13 C DIC ) were intermediate between local carbonate and organic C sources, but were higher than those of contemporaneous Pennsylvanian age groundwaters in the region. This suggests a significant contribution of C enriched in 13 C due to enhanced carbonate dissolution associated with the release of H 2 SO 4 from pyrite oxidation. The Sr isotopic signature of the drainage was similar to other regional mine waters associated with the same coal seam and reflected contributions from limestone dissolution and cation exchange with clay minerals. The relatively high d 34 S SO4 and d 18 O SO4 isotopic signatures of the mine drainage and the presence of presumptive SO 4 -reducing bacteria suggest that SO 4 reduction activity also contributes C depleted in 13 C isotope to the total DIC pool. With distance downstream from the mine portal, C isotope signatures in the drainage increased , accompanied by decreased total DIC concentrations and increased pH. These data are consistent with H 2 SO 4 dissolution of carbonate rocks, enhanced by cation exchange, and C release to the atmosphere via CO 2 outgassing.

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Flow Cytometry and Cell Sorting for Monitoring Microbial Cells

Deere

Vesey²,

Gauci³

et al. 2003

Encyclopedia of Environmental Microbiology

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An In Situ Hybridization Protocol for Detection and Identification of Planktonic Bacteria

Cited by 289 publications

References 19 publications

Changes in the Epilimnetic Bacterial Community Composition, Production, and Protist-Induced Mortality along the Longitudinal Axis of a Highly Eutrophic Reservoir

Changes in the Epilimnetic Bacterial Community Composition, Production, and Protist-Induced Mortality along the Longitudinal Axis of a Highly Eutrophic Reservoir

Isotopic evidence of enhanced carbonate dissolution at a coal mine drainage site in Allegheny County, Pennsylvania, USA

Flow Cytometry and Cell Sorting for Monitoring Microbial Cells

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