An in-vitro and in-vivo methodology study of alveolar bone measurement using extra-oral radiographic alignment apparatus, Image Pro-Plus® software and a subtraction programme

Rawlinson, Āndrew; Elcock, Claire; Cheung, Anthony; Al-Buhairi, A.; Khanna, Sangeeta; Walsh, T. F.; Ellwood, R.P.

doi:10.1016/j.jdent.2005.01.013

Cited by 17 publications

(11 citation statements)

References 20 publications

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“…Once in digital format the images were analyzed with Image-Pro Plus computer software (version 7.0) using a technique described previously. 14 A single operator carried out all the measurements on the masked images, which were repeated after two weeks. The repeatability of the methods was assessed using a paired t test to detect systematic error and an intra-class correlation coefficient to determine random error.…”

Section: Alveolar Bone Levelsmentioning

confidence: 99%

Periodontal health of palatally displaced canines treated with open or closed surgical technique: A multicenter, randomized controlled trial

Parkin

Milner

Deery

et al. 2013

American Journal of Orthodontics and Dentofacial Orthopedics

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Section: Alveolar Bone Levelsmentioning

confidence: 99%

Periodontal health of palatally displaced canines treated with open or closed surgical technique: A multicenter, randomized controlled trial

Parkin

Milner

Deery

et al. 2013

American Journal of Orthodontics and Dentofacial Orthopedics

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“…6 Threedimensional (3D) micro computed tomography (mCT) analysis has been used to overcome these limitations to obtain conclusive research outcomes. 1,[28][29][30] Although 3D Micro CT has been used by a few researchers to analyze mandibular bone, 31,32 it has not been used for analysis of maxillary bone changes due to the lack of method to extract the volume of interest (VOI) areas in maxilla. Therefore, this study was undertaken to compare maxillary and tibial bone morphometric parameter changes resulting from estrogen deficiency induced by ovariectomy.…”

mentioning

confidence: 99%

Estrogen Deficiency-Associated Bone Loss in the Maxilla: A Methodology to Quantify the Changes in the Maxillary Intra-radicular Alveolar Bone in an Ovariectomized Rat Osteoporosis Model

Steck

Doan

et al. 2015

Tissue Engineering Part C: Methods

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The effects of estrogen deficiency on bone characteristics are site-dependent, with the most commonly studied sites being appendicular long bones (proximal femur and tibia) and axial bones (vertebra). The effect on the maxillary and mandibular bones is still inconsistent and requires further investigation. This study was designed to evaluate bone quality in the posterior maxilla of ovariectomized rats to validate this site as an appropriate model to study the effect of osteoporotic changes. Forty-eight 3-month-old female Sprague-Dawley rats were randomly divided into two groups: an ovariectomized (OVX) group (n=24) and Sham-operated (SHAM) group (n=24). Six rats were randomly sacrificed from both groups at time points 8, 12, 16, and 20 weeks. The samples from tibia and maxilla were collected for micro computed tomography (μCT) and histological analysis. For the maxilla, the volume of interest area focused on the furcation areas of the first and second molar. Trabecular bone volume fraction (BV/TV, %), trabecular thickness (Tb.Th.), trabecular number (Tb.N.), trabecular separation (Tb.Sp.), and connectivity density (Conn.Dens) were analyzed after Micro CT scanning. At 8 weeks the indices BV/TV, Tb.Sp., Tb.N., and Conn.Dens showed significant differences (p<0.05) between the OVX and SHAM groups in the tibia. Compared with the tibia, the maxilla developed osteoporosis at a later stage, with significant changes in maxillary bone density only occurring after 12 weeks. Compared with the SHAM group, both the first and second molars of the OVX group showed significantly decreased BV/TV values from 12 weeks, and these changes were sustained through 16 and 20 weeks. For Tb.Sp., there were significant increases in bone values for the OVX group compared with the SHAM group at 12, 16, and 20 weeks. Histological changes were highly consistent with Micro CT results. This study established a method to quantify the changes of intra-radicular alveolar bone in the posterior maxilla in an accepted rat osteoporosis model. The degree of the osteoporotic changes to trabecular bone architecture is site-dependent and at least 3 months are required for the osteoporotic effects to be apparent in the posterior maxilla following rat OVX.

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“…The accuracy of the digital measurement methods has been examined in previous studies. As expected, the intraobserver reliability (intraclass correlation coefficient: 0.98) is more accurate than interobserver examination (0.69 and 0.65) [Shinkai et al, 2004;Rawlinson et al, 2005]. In this study, the Adobe Photoshop 7 was used to analyze the digital images.…”

Section: Discussionmentioning

confidence: 74%

“…Digital CT and X-ray images or histological sections are frequently used to quantify the amount of bone formation [Mohr et al, 2003;Shinkai et al, 2004;Rawlinson et al, 2005] Image Solutions IMSOL, Standish, Wigan (UK), Image Science Software, Berlin (Germany) and Imagic Bildverarbeitung, Glattbrugg (Switzerland)]. The accuracy of the digital measurement methods has been examined in previous studies.…”

Section: Discussionmentioning

confidence: 99%

Digital Measurements: A Different Approach to Evaluate Bone Formation

Gordjestani¹,

Dermaut²,

Ridder

et al. 2006

Cells Tissues Organs

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In this study a digital measurement technique has been proposed to quantify bone formation on histological images. Two standard parietal defects were created in 30 adult rabbits. The animals were divided into six groups. Four animals of each group were randomly chosen as experimental group in which osteopontin-coated hydroxyapatite (OPN-HA) and hydroxyapatite (HA) were inserted alternatively in created defects. To observe the spontaneous healing process of defects, one animal of each group was used as control animal and these created defects did not receive any implants. The animals were sacrificed after 1, 2, 6, 12, 18 and 30 weeks. The histological sections were magnified (×100) and scanned digitally. The newly formed bone surfaces within the healing area were indicated and quantified by means of Adobe Photoshop® 7 software. This measuring technique was found to be reliable and reproducible. The results of this study show no significant differences in bone formation between the OPN-HA and non-coated HA defects, although a significant difference in bone formation was measured at the margins of the defects treated with OPN-HA.

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An in-vitro and in-vivo methodology study of alveolar bone measurement using extra-oral radiographic alignment apparatus, Image Pro-Plus® software and a subtraction programme

Cited by 17 publications

References 20 publications

Periodontal health of palatally displaced canines treated with open or closed surgical technique: A multicenter, randomized controlled trial

Periodontal health of palatally displaced canines treated with open or closed surgical technique: A multicenter, randomized controlled trial

Estrogen Deficiency-Associated Bone Loss in the Maxilla: A Methodology to Quantify the Changes in the Maxillary Intra-radicular Alveolar Bone in an Ovariectomized Rat Osteoporosis Model

Digital Measurements: A Different Approach to Evaluate Bone Formation

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