An in vitro antifungal efficacy of silver nanoparticles activated by diode laser to Candida albicans

Astuti, Suryani Dyah; Kharisma, Ditya Hanif; Kholimatussadiah, Septia; Zaidan, Andi Hamim

doi:10.1063/1.5004293

Cited by 10 publications

(5 citation statements)

References 22 publications

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“…This difference in efficacy based on sizes is related to their large surface area to volume ratio, which allows efficient binding with the bacterial surface. Recently, researchers have discovered how to activate the bactericidal effect of Ag-NPs using laser irradiation [4,5], thus offering a promising development in the fight against antibacterial resistance. The size of metal particles (NPs) also correlates with their optical properties, which have been extensively studied and found to depend on their size, shape, composition, and dielectric surrounding medium [6].…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, a number of works have attempted to explore the toxicity of Ag-NPs after photoactivation using UV and visible laser light. These studies have shown that the antimicrobial effects of Ag-NPs can be enhanced through light activation [5]. Moreover, exposure of cells to Ag-NPs irradiated by low-power visible light can cause oxidative stress owing to the production of reactive oxygen species (ROS) such as, ⋅ OH, O 2 ⋅-, and ROS, produced by photoactive NPs which can damage cellular components, e.g., proteins and DNA of treated cells [11,12].…”

Section: Introductionmentioning

confidence: 99%

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Enhancement of the Antibacterial Efficiency of Silver Nanoparticles against Gram-Positive and Gram-Negative Bacteria Using Blue Laser Light

Al‐Sharqi¹,

Apun²,

Vincent³

et al. 2019

International Journal of Photoenergy

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Silver nanoparticles (Ag-NPs) possess excellent antibacterial properties and are considered to be an alternative material for treating antibiotic-resistant bacteria. The present study was aimed at enhancing the antibacterial efficiency of Ag-NPs using visible laser light against Escherichia coli and Staphylococcus aureus in vitro. Four concentrations of Ag-NPs (12.5, 25, 50, and 100 μg/ml), synthesized by the chemical reduction method, were utilized to conduct the antibacterial activity of prepared Ag-NPs. The antibacterial efficiencies of photoactivated Ag-NPs against both bacteria were determined by survival assay after exposure to laser irradiation. The mechanism of interactions between Ag-NPs and the bacterial cell membranes was then evaluated via scanning electron microscopy (SEM) and reactive oxygen species analysis to study the cytotoxic action of photoactivated Ag-NPs against both bacterial species. Results showed that the laser-activated Ag-NP treatment reduced the surviving population to 14% of the control in the E. coli population, while the survival in the S. aureus population was reduced to 28% of the control upon 10 min exposure time at the concentration of 50 μg/ml. However, S. aureus showed lower sensitivity after photoactivation compared to E. coli. Moreover, the effects depended on the concentration of Ag-NPs and exposure time to laser light. SEM images of treated bacterial cells indicated that substantial morphological changes occurred in cell membranes after treatment. The results suggested that Ag-NPs in the presence of visible light exhibit strong antibacterial activity which could be used to inactivate harmful and pathogenic microorganisms.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhancement of the Antibacterial Efficiency of Silver Nanoparticles against Gram-Positive and Gram-Negative Bacteria Using Blue Laser Light

Al‐Sharqi¹,

Apun²,

Vincent³

et al. 2019

International Journal of Photoenergy

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“…Action of chemical AgNPs synthesized using citrate reduction method against biofilms of C. albicans and C. glabrata has been described (Silva et al 2013). A study on synthesis of AgNPs from C. albicans with antifungal activity against C. albicans has also been reported (Astuti et al 2017). Chemically synthesized AgNPs exhibits low MIC values along with high toxicity and low stability (Pal et al 2007;Kittler et al 2010;Pereira et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Acinetobacter sp. mediated synthesis of AgNPs, its optimization, characterization and synergistic antifungal activity against C. albicans

Nadhe

Singh²,

Wadhwani

et al. 2019

J of Applied Microbiology

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Aims To synthesize silver nanoparticles (AgNPs) with cell free extract of Acinetobacter sp. and evaluate antifungal activity against planktonic and biofilm of Candida. Also, to study mechanism of antifungal action of AgNPs. Methods and Result Acinetobacter spp were screened for synthesis of AgNPs. Physio‐chemical parameters were optimized to obtained monodispersed nanoparticles. Optimized nanoparticles were characterized using spectroscopic, microscopic and diffraction techniques. Antifungal and biofilm disruption activity of AgNPs (10 ± 5 nm) were investigated against C. albicans. Mechanism of antifungal activity of nanosilver was deduced by growth curve, reactive oxygen species generation, thiol interaction and microscopic analysis. Acinetobacter sp. GWRFH 45 gave maximum synthesis of AgNPs. At optimized condition monodispersed, spherical nanoparticles were obtained which were crystalline with negative surface charge. AgNPs exhibited antifungal activity against planktonic cells and biofilm of Candida. AgNPs showed synergistic effect with amphotericin B as well as fluconazole against biofilm disruption. AgNPs were found to affect growth of Candida, generate reactive oxygen species and disrupt cellular morphology. Conclusions Cell free extract of A. calcoaceticus GWRFH 45 has ability to synthesize AgNPs. AgNPs alone and in combination with drugs have potential to inhibit C. albicans. Significance and Impact of the Study This is the first report of bacteriogenic AgNPs used in combination with antifungal drugs against Candida.

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“…Our results corroborated literature reports that observed minimal antimicrobial effect of AgNPs when short interaction times were used. Astuti et al 45 evaluated the inactivation of C. albicans yeasts assisted by AgNPs under blue laser irradiation (450 nm, IR time of 1.25 min, and 29.25 J/cm 2 ). Their results showed ca .…”

Section: Resultsmentioning

confidence: 99%

A Novel Strategy Based on Zn(II) Porphyrins and Silver Nanoparticles to Photoinactivate Candida albicans

Raposo¹,

Souza²,

Santana³

et al. 2023

IJN

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Background Photodynamic inactivation (PDI) is an attractive alternative to treat Candida albicans infections, especially considering the spread of resistant strains. The combination of the photophysical advantages of Zn(II) porphyrins (ZnPs) and the plasmonic effect of silver nanoparticles (AgNPs) has the potential to further improve PDI. Here, we propose the novel association of polyvinylpyrrolidone (PVP) coated AgNPs with the cationic ZnPs Zn(II) meso -tetrakis( N -ethylpyridinium-2-yl)porphyrin or Zn(II) meso -tetrakis( N -n-hexylpyridinium-2-yl)porphyrin to photoinactivate C. albicans . Methods AgNPs stabilized with PVP were chosen to allow for (i) overlap between the NP extinction and absorption spectra of ZnPs and (ii) favor AgNPs-ZnPs interaction; prerequisites for exploring the plasmonic effect. Optical and zeta potential (ζ) characterizations were performed, and reactive oxygen species (ROS) generation was also evaluated. Yeasts were incubated with individual ZnPs or their respective AgNPs-ZnPs systems, at various ZnP concentrations and two proportions of AgNPs, then irradiated with a blue LED. Interactions between yeasts and the systems (ZnP alone or AgNPs-ZnPs) were evaluated by fluorescence microscopy. Results Subtle spectroscopic changes were observed for ZnPs after association with AgNPs, and the ζ analyses confirmed AgNPs-ZnPs interaction. PDI using ZnP-hexyl (0.8 µM) and ZnP-ethyl (5.0 µM) promoted a 3 and 2 log 10 reduction of yeasts, respectively. On the other hand, AgNPs-ZnP-hexyl (0.2 µM) and AgNPs-ZnP-ethyl (0.6 µM) systems led to complete fungal eradication under the same PDI parameters and lower porphyrin concentrations. Increased ROS levels and enhanced interaction of yeasts with AgNPs-ZnPs were observed, when compared with ZnPs alone. Conclusion We applied a facile synthesis of AgNPs which boosted ZnP efficiency. We hypothesize that the plasmonic effect combined with the greater interaction between cells and AgNPs-ZnPs systems resulted in an efficient and improved fungal inactivation. This study provides insight into the application of AgNPs in PDI and helps diversify our antifungal arsenal, encouraging further developments toward inactivation of resistant Candida spp.

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An in vitro antifungal efficacy of silver nanoparticles activated by diode laser to Candida albicans

Cited by 10 publications

References 22 publications

Enhancement of the Antibacterial Efficiency of Silver Nanoparticles against Gram-Positive and Gram-Negative Bacteria Using Blue Laser Light

Enhancement of the Antibacterial Efficiency of Silver Nanoparticles against Gram-Positive and Gram-Negative Bacteria Using Blue Laser Light

Acinetobacter sp. mediated synthesis of AgNPs, its optimization, characterization and synergistic antifungal activity against C. albicans

A Novel Strategy Based on Zn(II) Porphyrins and Silver Nanoparticles to Photoinactivate Candida albicans

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