An in vitro characterization study of new near infrared dyes for molecular imaging

Pauli, Jutta; Vag, Tibor; Haag, Romy; Spieles, Monika; Wenzel, Matthias; Kaiser, Werner A.; Resch‐Genger, Ute; Hilger, Ingrid

doi:10.1016/j.ejmech.2009.01.019

Cited by 87 publications

(103 citation statements)

References 64 publications

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“…Several simple methods can be used to evaluate the activity of prepared fluorescent liposomes.DY-676-COOH has a high tendency to selfquench at high concentrations 21,29 probably resulting from H-dimer formation and pi-stacking interactions between dye molecules. These interactions, which occur due to nearness of the Förster radii of dye molecules at high concentrations, can be annihilated by dilution 30 .…”

Section: Discussionmentioning

confidence: 99%

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for <em>In Vivo</em> Optical Imaging

Tansi

Rüger

Rabenhold

et al. 2015

JoVE

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Optical imaging offers a wide range of diagnostic modalities and has attracted a lot of interest as a tool for biomedical imaging. Despite the enormous number of imaging techniques currently available and the progress in instrumentation, there is still a need for highly sensitive probes that are suitable for in vivo imaging. One typical problem of available preclinical fluorescent probes is their rapid clearance in vivo, which reduces their imaging sensitivity. To circumvent rapid clearance, increase number of dye molecules at the target site, and thereby reduce background autofluorescence, encapsulation of the near-infrared fluorescent dye, DY-676-COOH in liposomes and verification of its potential for in vivo imaging of inflammation was done. DY-676 is known for its ability to self-quench at high concentrations. We first determined the concentration suitable for self-quenching, and then encapsulated this quenching concentration into the aqueous interior of PEGylated liposomes. To substantiate the quenching and activation potential of the liposomes we use a harsh freezing method which leads to damage of liposomal membranes without affecting the encapsulated dye. The liposomes characterized by a high level of fluorescence quenching were termed Lip-Q. We show by experiments with different cell lines that uptake of Lip-Q is predominantly by phagocytosis which in turn enabled the characterization of its potential as a tool for in vivo imaging of inflammation in mice models. Furthermore, we use a zymosan-induced edema model in mice to substantiate the potential of Lip-Q in optical imaging of inflammation in vivo. Considering possible uptake due to inflammation-induced enhanced permeability and retention (EPR) effect, an always-on liposome formulation with low, non-quenched concentration of DY-676-COOH (termed LipdQ) and the free DY-676-COOH were compared with Lip-Q in animal trials. Video LinkThe video component of this article can be found at

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Section: Discussionmentioning

confidence: 99%

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for <em>In Vivo</em> Optical Imaging

Tansi

Rüger

Rabenhold

et al. 2015

JoVE

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“…20 Other types of anchoring moieties are also used for IONP pegylation, like nitrodopamine 21 or silanes 22,23 . In the present study, the near infrared (NIR) fluorescent dye DY700 that is stable under in vitro conditions and has low cytotoxicity 24 was also grafted using the phosphonate pre-functionalisation strategy in order to easily track the IONPs incubated with cells. A U87 human glioblastoma cell line, genetically modified to overexpress the bioluminescent reporter gene firefly luciferase (Fluc), was used to provide a convenient way to monitor the time course of cell viability by bioluminescence imaging (BLI) and to quantify the IONPs' performances in terms of magnetically-induced toxicity.…”

Section: Introductionmentioning

confidence: 99%

Monocorevs.multicore magnetic iron oxide nanoparticles: uptake by glioblastoma cells and efficiency for magnetic hyperthermia

Hemery

Genevois

Couillaud

et al. 2017

Mol. Syst. Des. Eng.

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“…The fluorescence quantum yield of ICG in water is about 4% [32] and is even less in blood [33]. This value is relatively low in comparison to specific dyes tested in−vitro in animals [34]. The multichannel time−resolved brain imager described in detail elsewhere [35] was reconstructed in such a way that it allows for fast acquisition of distributions of times of arrival of fluorescence (DTA) photons and distribu− tions of times of flight (DTOF) of diffusely reflected photons at multiple source−detector pairs located on the surface of the studied tissue phantom.…”

Section: Introductionmentioning

confidence: 98%

Time-resolved imaging of fluorescent inclusions in optically turbid medium — phantom study

Kacprzak

Liebert

Sawosz

et al. 2010

Opto-Electronics Review

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We present results of application of a time-resolved optical system for imaging of fluorescence excited in an inclusion containing indocyanine green (ICG), and located in optically turbid medium. The developed imaging system enabled simultaneous acquisition of fluorescence and diffusive reflectance. Eight independent time-resolved measurement channels based on time-correlated single photon counting technique were applied. In four of these channels, used for the fluorescence detection, sets of filters were applied in order to block the excitation light. Fast optomechanical switches allowed us to illuminate sequentially nine different spots on the surface of the studied object and finally 4×4 pixels maps at excitation and emission wavelengths were obtained. A liquid phantom used in this study consists of the fish tank filed with a solution ofmilk and water with black ink added to obtain optical properties in the range of the optical properties typical for the living tissue. A gel ball of a diameter of 5 mm with precisely controlled concentration of ICG was immersed in the liquid. The measurements were performed for inclusion located at different depths and for various ICG concentrations in the gel ball and in the surrounding liquid. The recorded distributions of times of arrival (DTA) of fluorescence photons and times of flight (DTOF) of diffusely reflected photons were analyzed by calculation of their statistical moments. We observed specific changes in moments of the measured DTAs as a function of depth of immersion of the fluorescent inclusion in the medium. We noted also that the changes of moments depend significantly on concentration of the dye in the fluorescence inclusion as well as in the surrounding liquid.

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An in vitro characterization study of new near infrared dyes for molecular imaging

Cited by 87 publications

References 64 publications

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for <em>In Vivo</em> Optical Imaging

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for <em>In Vivo</em> Optical Imaging

Monocorevs.multicore magnetic iron oxide nanoparticles: uptake by glioblastoma cells and efficiency for magnetic hyperthermia

Time-resolved imaging of fluorescent inclusions in optically turbid medium — phantom study

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