An In Vitro Estimation of the Cytotoxicity and Genotoxicity of Root Extract from Leonurus sibiricus L. Overexpressing AtPAP1 against Different Cancer Cell Lines

Sitarek, Przemysław; Synowiec, Ewelina; Kowalczyk, Tomasz; Śliwiński, Tomasz; Skała, Ewa

doi:10.3390/molecules23082049

Cited by 18 publications

(20 citation statements)

References 42 publications

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“…Many studies have shown that plant extracts or pure compounds isolated from plants are able to induce apoptosis in various types of cancer [22,[30][31][32]. Although revolutions in the modern drug industry have given rise to a trend of replacing herbal remedies with modern drugs, studies of natural compounds from herbal plants and their activities are still of interest for drug discovery; they are not only characterized by better absorption but also by lower toxicity than chemical drugs.…”

Section: Discussionmentioning

confidence: 99%

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Insight the Biological Activities of Selected Abietane Diterpenes Isolated from Plectranthus spp.

et al. 2020

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Natural compounds isolated from plants are excellent starting points in drug design and have been widely studied as anticancer agents; they hence find use in a considerable proportion of anticancer drugs. The genus Plectranthus (Lamiaceae) comprises a large and widespread group of species with various applications in traditional medicine. Therefore, the aim of the present study was to determine the effectiveness of treatment with four abietane diterpenoids isolated from P. madagascariensis and P. ecklonii, 6,7-dehydroroyleanone, 7β,6β-dihydroxyroyleanone, 7α-acetoxy-6β-hydroxyroyleanone and parvifloron D, in initiating apoptosis in a glioma cell line. The pure compounds were found to exhibit anticancer effects in primary H7PX glioma cells line by inducing apoptosis G2/M cell cycle arrest and double-strand breaks, indicated by increased levels of phosphorylated H2A.X and decreasing mitochondrial membrane potential; they also influenced the expression of pro- and anti-apoptotic genes (Bax, Bcl-2, TP53, or Cas-3). Our findings indicate that these compounds may offer potential as beneficial antitumor drugs but further in vivo studies are needed.

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Section: Discussionmentioning

confidence: 99%

“…Prior to measurements, the cells were centrifuged (300× g for 10 min at 22 • C) then washed twice with the HBSS. Further procedure has been described previously [22,23].…”

Section: Mitochondrial Membrane Potential (Mmp)mentioning

confidence: 99%

Insight the Biological Activities of Selected Abietane Diterpenes Isolated from Plectranthus spp.

et al. 2020

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“…The cell growing process was performed in a humidified incubator at 37 • C and 5% CO 2 . To perform the analysis cells were seeded at 3 × 10 6 cells per well and were left in the incubator for 12 h before treatment procedures [34]. Next, the cell samples were incubated with melittin in a concentration range of 0.001 to 100 µM for 24 h and 48 h. As a positive control (S)-(+)-camptothecin was used in three different concentration-10, 50 and 100 µM.…”

Section: Cell Culturesmentioning

confidence: 99%

Melittin—A Natural Peptide from Bee Venom Which Induces Apoptosis in Human Leukaemia Cells

et al. 2020

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Bee venom is a very complex mixture produced and secreted by the honeybee (Apis mellifera). Melittin is a major component of bee venom that accounts for about 52% of its dry mass. A vast number of studies have been dedicated to the effects of melittin’s regulation of apoptosis and to the factors that induce apoptosis in various types of cancer such as breast, ovarian, prostate, lung. The latest evidence indicates its potential as a therapeutic agent in the treatment of leukaemia. The aim of our present study is to evaluate melittin’s ability to induce apoptosis in leukaemia cell lines of different origin acute lymphoblastic leukaemia (CCRF-CEM) and chronic myelogenous leukaemia (K-562). We demonstrated that melittin strongly reduced cell viability in both leukaemia cell lines but not in physiological peripheral blood mononuclear cells (PMBCs). Subsequent estimated parameters (mitochondrial membrane potential, Annexin V binding and Caspases 3/7 activity) clearly demonstrated that melittin induced apoptosis in leukaemia cells. This is a very important step for research into the development of new potential anti-leukaemia as well as anticancer therapies. Further analyses on the molecular level have been also planned (analysis of proapoptotic genes expression and DNA damages) for our next research project, which will also focus on melittin.

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“…Prior to measurements, the cells were centrifuged (300× g for 10 min at 22 • C) then washed twice with the HBSS. Finally, the fluorescence was measured on a Bio-Tek Synergy HT Microplate Reader according to our previous study [24].…”

Section: Mitochondrial Membrane Potential (∆ψM)mentioning

confidence: 99%

An Extract of Transgenic Senna obtusifolia L. hairy roots with Overexpression of PgSS1 Gene in Combination with Chemotherapeutic Agent Induces Apoptosis in the Leukemia Cell Line

et al. 2020

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Many biologically-active plant-derived compounds have therapeutic or chemopreventive effects. The use of plant in vitro cultures in conjunction with modern genetic engineering techniques allows greater amounts of valuable secondary metabolites to be obtained without interfering with the natural environment. This work presents the first findings concerning the acquisition of transgenic hairy roots of Senna obtusifolia overexpressing the gene encoding squalene synthase 1 from Panax ginseng (PgSS1) (SOPSS hairy loot lines) involved in terpenoid biosynthesis. Our results confirm that one of PgSS1-overexpressing hairy root line extracts (SOPSS2) possess a high cytotoxic effect against a human acute lymphoblastic leukemia (NALM6) cell line. Further analysis of the cell cycle, the expression of apoptosis-related genes (TP53, PUMA, NOXA, BAX) and the observed decrease in mitochondrial membrane potential also confirmed that the SOPSS2 hairy root extract displays the highest effects; similar results were also obtained for this extract combined with doxorubicin. The high cytotoxic activity, observed both alone or in combination with doxorubicin, may be due to the higher content of betulinic acid as determined by HPLC analysis. Our results suggest synergistic effects of tested extract (betulinic acid in greater amount) with doxorubicin which may be used in the future to develop new effective strategies of cancer chemosensitization.

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An In Vitro Estimation of the Cytotoxicity and Genotoxicity of Root Extract from Leonurus sibiricus L. Overexpressing AtPAP1 against Different Cancer Cell Lines

Cited by 18 publications

References 42 publications

Insight the Biological Activities of Selected Abietane Diterpenes Isolated from Plectranthus spp.

Insight the Biological Activities of Selected Abietane Diterpenes Isolated from Plectranthus spp.

Melittin—A Natural Peptide from Bee Venom Which Induces Apoptosis in Human Leukaemia Cells

An Extract of Transgenic Senna obtusifolia L. hairy roots with Overexpression of PgSS1 Gene in Combination with Chemotherapeutic Agent Induces Apoptosis in the Leukemia Cell Line

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