An in vitro evaluation of the cell toxicity of honey and silver dressings

Toit, D. F. Du; Page, Benedict J.

doi:10.12968/jowc.2009.18.9.44307

Cited by 46 publications

(28 citation statements)

References 25 publications

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“…Our findings are consistent with previous investigators who have reported diminished proliferation of keratinocytes in the presence of silver ions. [24][25][26] In the absence of bacteria in the culture medium, alamarBlue and DNA content findings indicated that keratinocytes exhibited a significantly greater proliferation on AgMP-loaded scaffolds relative to AgNP-loaded scaffolds. However, with the inclusion of S. aureus bacteria in the coculture system, there was no significant difference in keratinocyte cytotoxicity between AgMP or AgNP scaffolds according to DNA content analyses.…”

Section: Discussionmentioning

confidence: 99%

Skin Tissue Engineering for the Infected Wound Site: Biodegradable PLA Nanofibers and a Novel Approach for Silver Ion Release Evaluated in a 3D Coculture System of Keratinocytes and Staphylococcus aureus

Mohiti-Asli

Pourdeyhimi

Loboa

2014

Tissue Engineering Part C: Methods

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Wound infection presents a challenging and growing problem. With the increased prevalence and growth of multidrug-resistant bacteria, there is a mounting need to reduce and eliminate wound infections using methodologies that limit the ability of bacteria to evolve into further drug-resistant strains. A well-known strategy for combating bacterial infection and preventing wound sepsis is through the delivery of silver ions to the wound site. High surface area silver nanoparticles (AgNPs) allowing extensive silver ion release have therefore been explored in different wound dressings and/or skin substitutes. However, it has been recently shown that AgNPs can penetrate into the stratum corneum of skin or diffuse into the cellular plasma membrane, and may interfere with a variety of cellular mechanisms. The goal of this study was to introduce and evaluate a new type of high surface area metallic silver in the form of highly porous silver microparticles (AgMPs). Polylactic acid (PLA) nanofibers were successfully loaded with either highly porous AgMPs or AgNPs and the antimicrobial efficacy and cytotoxicity of the two silver-based wound dressings were assessed and compared. To better mimic the physiological environment in vivo where both human cells and bacteria are present, a novel coculture system combining human epidermal keratinocytes and Staphylococcus aureus bacteria was designed to simultaneously evaluate human skin cell cytotoxicity with antimicrobial efficacy in a three-dimensional environment. We found that highly porous AgMPs could be successfully incorporated in nanofibrous wound dressings, and exhibited comparable antimicrobial efficacy and cytotoxicity to AgNPs. Further, PLA nanofibers containing highly porous AgMPs exhibited steady silver ion release, at a greater rate of release, than nanofibers containing AgNPs. The replacement of AgNPs with the newly introduced AgMPs overcomes concerns regarding the use of nanoparticles and holds great promise as skin substitutes or wound dressings for infected wound sites.

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Section: Discussionmentioning

confidence: 99%

Skin Tissue Engineering for the Infected Wound Site: Biodegradable PLA Nanofibers and a Novel Approach for Silver Ion Release Evaluated in a 3D Coculture System of Keratinocytes and Staphylococcus aureus

Mohiti-Asli

Pourdeyhimi

Loboa

2014

Tissue Engineering Part C: Methods

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“…Recently, the medical community has been recognizing the potential applications of honey [64][65][66][67]. Honey is the oldest wound dressing material used by humans and has traditionally been used to treat various conditions, including burns, infected and non-healing wounds, ulcers and boils [64][65][66][67]. This is due to honey's anti-bacterial capability, its high acidity, osmotic effects, anti-oxidant and hydrogen peroxide content and its stimulation of immunity, as well as other currently unidentified compounds [64].…”

Section: Flowering Shrubs As a Substitute Or Supplement To Trees In Amentioning

confidence: 99%

“…This is due to honey's anti-bacterial capability, its high acidity, osmotic effects, anti-oxidant and hydrogen peroxide content and its stimulation of immunity, as well as other currently unidentified compounds [64]. Sufficient evidence exists to recommend its use in the treatment of acute wounds and mild to moderate burns [64,66,67]. These types of medical applications have been found in multiple kinds of honey, including Gelam, Medihoney, Tualang and Manuka [64].…”

Section: Flowering Shrubs As a Substitute Or Supplement To Trees In Amentioning

confidence: 99%

Increasing the Effectiveness of the “Great Green Wall” as an Adaptation to the Effects of Climate Change and Desertification in the Sahel

O’Connor

Ford

2014

Sustainability

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Abstract:The Great Green Wall (GGW) has been advocated as a means of reducing desertification in the Sahel through the planting of a broad continuous band of trees from Senegal to Djibouti. Initially proposed in the 1980s, the plan has received renewed impetus in light of the potential of climate change to accelerate desertification, although the implementation has been lacking in all but two of 11 countries in the region. In this paper, we argue that the GGW needs modifying if it is to be effective, obtain the support of local communities and leverage international support. Specifically, we propose a shift from planting trees in the GGW to utilizing shrubs (e.g., Leptospermum scoparium, Boscia senegalensis, Grewia flava, Euclea undulata or Diospyros lycioides), which would have multiple benefits, including having a faster growth rate and proving the basis for silvo-pastoral livelihoods based on bee-keeping and honey production.

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“…Chlorhexidine also shows dose-dependent toxicity to ibroblasts at concentrations between 0.2 and 0.001% [53,54]. Moreover, silver-impregnated dressings have been reported to be more cytotoxic to epidermal keratinocytes and dermal ibroblasts than honey-based dressings [55]. On the other hand, antibiotic dressings (Table ) are nontoxic and can work efectively on the target sites without damaging host tissues [4 ].…”

Section: Advanced Medicated Antimicrobial Wound Dressingsmentioning

confidence: 99%

Antimicrobial Dressings for Improving Wound Healing

Sarheed¹,

Ahmed²,

Shouqair³

et al. 2016

Wound Healing - New Insights Into Ancient Challenges

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Wound healing occurs by a series of interrelated molecular events which work together to restore tissue integrity and cellular function. These physiological events occur smoothly in normal healthy individual and/or under normal conditions. However, in certain cases, these molecular events are retarded resulting in hard-to-heal or chronic wounds arising from several factors such as poor venous return, underlying physiological or metabolic conditions such as diabetes as well as external factors such as poor nutrition. In most cases, such wounds are infected and infection also presents as another complicating phenomenon which triggers inlammatory reactions, therefore delaying wound healing. There has therefore been recent interests and signiicant eforts in preventing and actively treating wound infections by directly targeting infection causative agents through direct application of antimicrobial agents either alone or loaded into dressings (medicated). These have the advantage of overcoming challenges such as poor circulation in diabetic and leg ulcers when administered systemically and also require lower amounts to be applied compared to that required via oral or iv administration. This chapter will review and evaluate various antimicrobial agents used to target infected wounds, the means of delivery, and current state of the art, including commercially available dressings. Data sources will include mainly peer-reviewed literature, clinical trials and reports, patents as well as government reports where available.

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An in vitro evaluation of the cell toxicity of honey and silver dressings

Cited by 46 publications

References 25 publications

Skin Tissue Engineering for the Infected Wound Site: Biodegradable PLA Nanofibers and a Novel Approach for Silver Ion Release Evaluated in a 3D Coculture System of Keratinocytes and Staphylococcus aureus

Skin Tissue Engineering for the Infected Wound Site: Biodegradable PLA Nanofibers and a Novel Approach for Silver Ion Release Evaluated in a 3D Coculture System of Keratinocytes and Staphylococcus aureus

Increasing the Effectiveness of the “Great Green Wall” as an Adaptation to the Effects of Climate Change and Desertification in the Sahel

Antimicrobial Dressings for Improving Wound Healing

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