2017
DOI: 10.1016/j.jmb.2017.04.016
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An in vitro Model to Mimic Selection of Replication-Competent HIV-1 Intersubtype Recombination in Dual or Superinfected Patients

Abstract: The low frequency of HIV-1 recombinants within entire viral populations in both individual patients and in culture-based infection models impedes investigation of the underlying factors contributing either to the occurrence of recombinants or the survival of recombinants once they are formed. So far, most of the related studies have no consideration of recombinants’ functionality. Here we established a Functional Recombinant Production (FRP) system to produce pure and functional HIV-1 intersubtype Env recombin… Show more

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Cited by 5 publications
(4 citation statements)
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“…Hence, even if recombination among subtypes has no net effect on virus fitness, it is reasonable for recombinants of particular subtypes (e.g., A and D) to be, on average, less fit or more fit than their respective parental variants. Several groups have constructed inter-subtype recombinants and performed functional assays or competitive growth experiments in vitro against the parental strains [38, 39]. Overall, the experimental results are equivocal with some recombinants displaying a slight but significant fitness advantage over the parental strains, while other recombinants are less fit [40].…”
Section: Discussionmentioning
confidence: 99%
“…Hence, even if recombination among subtypes has no net effect on virus fitness, it is reasonable for recombinants of particular subtypes (e.g., A and D) to be, on average, less fit or more fit than their respective parental variants. Several groups have constructed inter-subtype recombinants and performed functional assays or competitive growth experiments in vitro against the parental strains [38, 39]. Overall, the experimental results are equivocal with some recombinants displaying a slight but significant fitness advantage over the parental strains, while other recombinants are less fit [40].…”
Section: Discussionmentioning
confidence: 99%
“…In contrast to an abundance of field data, few experimental data are available concerning NoV recombination and the mechanism(s) involved remain poorly characterised [ 26 , 28 , 29 , 30 , 50 ]. An incremental step in the generation of any recombinant viral RNA and consequently any viable recombinant virus is the successful simultaneous infection of a single cell by (a minimum of) two viruses [ 32 , 33 , 34 , 35 ]. Under natural conditions, various environmental, host, and virus factors may influence the probability of synchronous coinfections and may determine the delay or even the absolute achievability of asynchronous cellular superinfections.…”
Section: Discussionmentioning
confidence: 99%
“…Following this, host coinfection, single cell coinfection, and recombination must be accomplished to generate a recombinant NoV RNA. An incipient recombinant viruses must then survive a process of functional selection to be maintained in the viral population [ 32 , 33 , 34 , 35 ]. The rise of recombinant viruses resulting from this process is influenced by different factors.…”
Section: Introductionmentioning
confidence: 99%
“…We asked whether the alteration of Phe 317 to the less-common tyrosine residue would alter CD4mc sensitivity in other HIV-1 strains. We introduced the F317Y change into the Envs of HIV-1 191084 , another clade A virus, and HIV-1 CRF02_AG253.11 , a Tier-3 clade AG recombinant virus (69,(73)(74)(75). In both HIV-1 strains, the F317Y change resulted in increased sensitivity to the CD4mcs (Fig.…”
Section: The Gp120 V3 Loop As a Determinant Of Cd4mc Susceptibility T...mentioning
confidence: 99%