An In Vitro Protocol for Propagating Castanea sativa Italian Cultivars

Pavese, Vera; Ruffa, Paola; Abbà, Simona; Costa, Rita; Corredoira, Elena; Silvestri, Cristian; Marinoni, Daniela Torello; Botta, Roberto

doi:10.3390/plants11233308

Cited by 8 publications

(5 citation statements)

References 44 publications

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“…To propagate the first shoots a mixture of BAR 0.1 mg + IBA 0.35 mg + GA3 0.2 mg / 1 L DKW is used, which will give each of them three new plants. 4. For good root formation, an environment is used in which three hormones are introduced -IBA 1.0 mg + NAA 0.5 mg + IAA 0.5 mg / l L DKW; this takes up to 30 days.…”

Section: Stage Materials Usedmentioning

confidence: 99%

“…Traditional methods are ineffective for large-scale reproduction of this plant for programs for the rapid renewal of natural or production sites or breeding in order to create disease-resistant germplasm [3,4,5]. In vitro reproduction is a highly effective tool for rapid and large-scale cloning of healthy germplasm from limited plant material under controlled environmental conditions, as well as for long-term maintenance of germplasm [6,7].…”

Section: Introductionmentioning

confidence: 99%

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Propagatıon of Azerbaıjanı Chestnut by Tıssue Culture

Hafizov,

Balazade

2023

Preprint

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Castanea sativa Mill., an indigenous species of the mountainous Gabala region of Azerbaijan, where its local variety is now facing the danger of extinction. The preservation of this variety of European chestnut requires the development of effective strategies for reliable in vitro regeneration systems as an alternative to traditional methods, which has become the main objective of this study. In solving this problem, the generally accepted technique of micro-multiplication of axillary shoots was mainly used. First, a phased sterilization of was carried out using liquid soap, Previkur fungicide and mercury (II) chloride. DKW (Driver and Kuniyuki Walnut) nutrient medium was used for germination of explants, into which growth stimulants BAP (Benzilamunapurine), IBA (Indole - 3 butyric acid), IAA (Indole Acetic acid), NAA (Naphthalene Acetic Acid) and GA3 (Gibberelic acid) were introduced in various combinations and quantities. The test of the above-mentioned sterilization model revealed significant shortcomings in terms of the acceptability of the results obtained (16-77%). It was also found that the germination of explants takes 14 days and it is better to conduct it in a DKW environment containing hormones BAP (0.6 mg), IBA (0.1 mg) and GA3 (0.1 mg) / 1 L DKW. A mixture of BAP (0.1mg) + IBA (0.35 mg) is more suitable for the reproduction of grown explants + GA3 (0.2 mg) / 1 l DKW (the result is 3 new micro-plants for each explant), and for good root formation (it takes 30 days) – a mixture of IBA 1.0 mg + NAA 0.5 mg + IAA 0.5 mg / l L DKW. After the shoots have acquired a certain length (at least 1.5 cm), it is required to transfer them for 22 days to a DKW medium containing IBA (1 mg), IAA (0.5 mg) and NAA(0.5 mg) / l L DKW so that the root splitting process begins and ends.

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Section: Stage Materials Usedmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Propagatıon of Azerbaıjanı Chestnut by Tıssue Culture

Hafizov,

Balazade

2023

Preprint

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“…Traditional methods are ineffective for large-scale reproduction of this plant for programs for the rapid renewal of natural or production sites or breeding in order to create disease-resistant germplasm (Liu et al, 2022;Pavese, et al, 2022;Troch et al, 2010). In vitro reproduction is a highly effective tool for rapid and largescale cloning of healthy germplasm from limited plant material under controlled environmental conditions, as well as for long-term maintenance of germplasm (Mullins, 1987;Tafazoli et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Propagation of Azerbaijani chestnut by tissue culture

Balazade,

Hafizov

2024

AFRJBS

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Castanea sativa Mill., an indigenous species of the mountainous Gabala region of Azerbaijan, where its local variety is now facing the danger of extinction. The preservation of this variety of European chestnut requires the development of effective strategies for reliable in vitro regeneration systems as an alternative to traditional methods, which has become the main objective of this study. In solving this problem, the generally accepted technique of micro-multiplication of axillary shoots was mainly used. First, a phased sterilization was carried out using liquid soap, Previkur fungicide and mercury (II) chloride. DKW (Driver and Kuniyuki Walnut) nutrient medium was used for germination of explants, into which growth stimulants BAP (Benzilamunapurine), IBA (Indole -3 butyric acid), IAA (Indole Acetic acid), NAA (Naphthalene Acetic Acid) and GA3 (Gibberelic acid) were introduced in various combinations and quantities. The test of the abovementioned sterilization model revealed significant shortcomings in terms of the acceptability of the results obtained (16-77%). It was also found that the germination of explants takes 14 days and it is better to conduct it in a DKW environment containing hormones BAP (0.6 mg), IBA (0.1 mg) and GA3 (0.1 mg)/1 L DKW. A mixture of BAP (0.1 mg) + IBA (0.35 mg) is more suitable for the reproduction of grown explants + GA3 (0.2 mg)/1 l DKW (the result is 3 new micro-plants for each explant), and for good root formation (it takes 30 days) -a mixture of IBA 1.0 mg + NAA 0.5 mg + IAA 0.5 mg/l L DKW. After the shoots have acquired a certain length (at least 1.5 cm), it is required to transfer them for 22 days to a DKW medium containing IBA (1 mg), IAA (0.5 mg) and NAA(0.5 mg)/l L DKW so that the root splitting process begins and ends.

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“…Conversely, the storage of desiccation-sensitive seeds (recalcitrant category), such as oak acorns, remains challenging [ 3 ]. When it is not possible to store seeds, the in vitro method allows for the preservation of endangered or valuable plant material via plant tissue cultures of valuable individuals, in addition to their regeneration and reintroduction to the natural environment [ 4 , 5 , 6 ]. For agricultural species, artificial seeds are produced by encapsulating somatic embryos, shoot tips, or any other micropropagule which has the ability to convert into a plant in vitro or ex vitro [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Successful In Vitro Shoot Multiplication of Quercus robur L. Trees Aged up to 800 Years

Chmielarz

Kotlarski

Kalemba

et al. 2023

Plants

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The conservation of the genetic resources of old trees is crucial to their ecological role but is extremely difficult, especially for oak species (Quercus spp.) displaying recalcitrance in seed and vegetative propagation methods. Our study aimed to assess the regenerative potential of Quercus robur trees of different ages (up to 800 years) during micropropagation. We also aimed to determine how in vitro conditions can influence in vitro regeneration responses. Lignified branches collected from 67 selected trees were cultivated ex vitro in culture pots at 25 °C to obtain epicormic shoots (explant sources). The explants were cultivated on an agar medium supplemented with 0.8 mg L−1 6-benzylaminopurine (BAP) for at least 21 months. In a second experiment, two different shoot multiplication conditions (temporary immersion—RITA® bioreactor and agar medium) and two culture medium formulations (Woody Plant Medium and modified Quoirin and Lepoivre medium) were tested. The results showed that the mean length of the epicormic shoots obtained in a pot culture was a function of donor age and was similar among the group of younger trees (ca. 20–200 years), and varied between older trees (ca. 300–800 years). The efficiency of in vitro shoot multiplication strictly depended on the genotype. A sustainable in vitro culture (defined as survival after 6 months) was only possible for half of the tested old donor trees, even when they survived the first month of in vitro growth. A continuous monthly increase in the number of in vitro cultured shoots was reported in younger oaks and in some old oaks. We found a significant effect of the culture system and the macro- and micronutrient composition on in vitro shoot growth. This is the first report demonstrating that the in vitro culture can be successfully applied to the propagation of even 800-year-old pedunculate oak trees.

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An In Vitro Protocol for Propagating Castanea sativa Italian Cultivars

Cited by 8 publications

References 44 publications

Propagatıon of Azerbaıjanı Chestnut by Tıssue Culture

Propagatıon of Azerbaıjanı Chestnut by Tıssue Culture

Propagation of Azerbaijani chestnut by tissue culture

Successful In Vitro Shoot Multiplication of Quercus robur L. Trees Aged up to 800 Years

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