An in Vitro Study of Syzygium Cumini Seed Extract on Glucose Uptake Activity in L-6 Cell Lines

Abstract

Diabetes mellitus is the most common endocrine disorder. The plant Syzygium cumini has been used in traditional medicine for the treatment of diabetes. The present study investigated the effect of ethanol extract of S. cumini seeds on uptake of glucose by L-6 rat skeletal muscle cells. S. cumini seeds were extracted with varying solvents and quantitative phytochemical analysis was carried out, ethanol extract of seeds exhibited higher content of tested phytochemicals. The effect of different concentrations (300µg/ml – 1000µg/ml) of ethanol extract of seeds were studied on glucose uptake activity of L-6 rat skeletal muscle cells. It was observed that with the increase in concentration, the glucose uptake activity was also increased. The results of the study supports and demonstrates the antidiabetic potential of ethanol seed extracts of Syzygium cumini utilizing in vitro model.
 KEY WORDS: Diabetes mellitus, Syzygium cumini, phytochemicals, glucose uptake, L-6 cells

In vitro anti-diabetic activity, bioactive constituents, and molecular modeling studies with sulfonylurea receptor1 for insulin secretagogue activity of seed extract of Syzygium cumini (L.)

“…SCE showed a concentration-dependent increase in glucose uptake activity, and the maximum activity was observed at 40 µg/mL with 19.91% uptake. Earlier studies on methanolic and ethanolic seed extracts of S. cumini have demonstrated a similar increase in the glucose uptake activity in L6 cell lines through GLUT4 translocation (11,22). The observed glucose uptake activity was attributed to the presence of polyphenols present in these extracts.…”