An In Vitro Study of Different Extracts and Fractions of Allium sativum (Garlic): Vascular Reactivity

Ganado, Patricia; Sanz, María del Mar Mateos; Padilla, Eugenia; Tejerina, Teresa

doi:10.1254/jphs.94.434

Cited by 13 publications

(5 citation statements)

References 20 publications

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“…In vitro studies strongly suggest that garlic has the ability to reduce some events associated with cardiovascular disease 27–29. Volatile organosulphur compounds, named thiosulfinates, are responsible for some of its bioactive actions 30.…”

Section: Discussionmentioning

confidence: 99%

Garlic components inhibit angiotensin II‐induced cell‐cycle progression and migration: Involvement of cell‐cycle inhibitor p27^Kip1 and mitogen‐activated protein kinase

Castro

Lorenzo

González

et al. 2010

Molecular Nutrition Food Res

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Garlic has been used for prevention and treatment of hypertension; however, the molecular mechanisms of garlic's effects remain to be elucidated. In this study, the mechanisms of the in vitro effect of organosulphur compounds derived from garlic on growth and migration of cultured aortic smooth muscle cells isolated from spontaneously hypertensive rats were investigated. We demonstrated that allyl methyl sulphide (AMS) and diallyl sulphide (DAS) inhibited aortic smooth muscle cell angiotensin II-stimulated cell-cycle progression and migration. Neither cell viability nor annexin-V-binding analysis revealed cytotoxic effects of both organosulphur compounds at the used concentrations. Instead, their inhibitory effects were associated to the prevention of the cell-cycle inhibitor p27(Kip1) (p27) downregulation and the reduction of extracellular signal-regulated kinase 1/2 phosphorylation. When we assessed the antioxidant activity of AMS and DAS, we found that both organosulphur compounds inhibited angiotensin II-reactive oxygen species generation. Our findings show that AMS and DAS, compounds derivate from garlic, could be effective antioxidants targeted at the arterial remodelling seen in hypertension.

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Section: Discussionmentioning

confidence: 99%

Garlic components inhibit angiotensin II‐induced cell‐cycle progression and migration: Involvement of cell‐cycle inhibitor p27^Kip1 and mitogen‐activated protein kinase

Castro

Lorenzo

González

et al. 2010

Molecular Nutrition Food Res

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“…Garlic extract and its derivatives have hypotensive properties and can induce vasodilation in vivo and in vitro (6,(23)(24)(25). We therefore asked whether these compounds evoke vasodilation through a TRPA1-dependent mechanism.…”

Section: Garlic Compounds Act On Sensory Nerve Fibers To Produce Vasomentioning

confidence: 99%

Pungent products from garlic activate the sensory ion channel TRPA1

Bautista

Movahed

Hinman

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

758

585

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Garlic belongs to the Allium family of plants that produce organosulfur compounds, such as allicin and diallyl disulfide (DADS), which account for their pungency and spicy aroma. Many health benefits have been ascribed to Allium extracts, including hypotensive and vasorelaxant activities. However, the molecular mechanisms underlying these effects remain unknown. Intriguingly, allicin and DADS share structural similarities with allyl isothiocyanate, the pungent ingredient in wasabi and other mustard plants that induces pain and inflammation by activating TRPA1, an excitatory ion channel on primary sensory neurons of the pain pathway. Here we show that allicin and DADS excite an allyl isothiocyanate-sensitive subpopulation of sensory neurons and induce vasodilation by activating capsaicin-sensitive perivascular sensory nerve endings. Moreover, allicin and DADS activate the cloned TRPA1 channel when expressed in heterologous systems. These and other results suggest that garlic excites sensory neurons primarily through activation of TRPA1. Thus different plant genera, including Allium and Brassica, have developed evolutionary convergent strategies that target TRPA1 channels on sensory nerve endings to achieve chemical deterrence.inflammation ͉ pain ͉ TRP channel ͉ vasodilation ͉ natural products

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“…According to previous investigations, garlic and its derivatives can reduce blood pressure by stimulation of NO production (Takashima, Kanamori, Kodera, Morihara, & Tamura, 2017), Na/H exchanger‐1 suppression (Al‐Qattan, Khan, Alnaqeeb, & Ali, 2003), enhancement of vascular hydrogen sulfide (Ried & Fakler, 2014) and alteration of extracellular/intracellular calcium exchange (Ganado, Sanz, Padilla, & Tejerina, 2004).…”

Section: Discussionmentioning

confidence: 98%

Evaluating the effects of dark chocolate formulated with micro‐encapsulated fermented garlic extract on cardio‐metabolic indices in hypertensive patients: A crossover, triple‐blind placebo‐controlled randomized clinical trial

Mohammadi

Mazloomi

Niakousari

et al. 2022

Phytotherapy Research

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This study aimed to investigate the health‐related effects of microencapsulated fermented garlic extract (FGE) containing dark chocolate in hypertensive adults. For this purpose, 36 hypertensive adults (15 males vs. 21 females) were randomized to receive the FGE (5 g/day) dark chocolate containing 650 mg of FGE powder or the placebo. Intervention periods lasted for 6 weeks and were separated by a 3‐week wash‐out period. The response variables included blood pressure, anthropometric indices, lipid profile, and inflammatory and oxidative stress indices. Statistical analyses were performed using the Pkcross procedure, and Cohen's d was estimated for all response variables. There was no significant inter‐period difference between the mean changes of body weight, waist circumference, and body mass index (BMI). Furthermore, no significant change was confirmed in participants' blood pressure, triglycerides, total cholesterol, low‐density lipoprotein (LDL), high‐density lipoprotein (HDL), very low‐density lipoprotein (VLDL), serum homocysteine, high‐sensitive c‐reactive protein (hs‐CRP), malondialdehyde (MDA), and total antioxidant capacity (TAC). It seems that the dose of FGE used in this study was not sufficient to cause any significant changes in the outcomes. Therefore, further studies with dose–response designs and longer durations are recommended.

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An In Vitro Study of Different Extracts and Fractions of Allium sativum (Garlic): Vascular Reactivity

Cited by 13 publications

References 20 publications

Garlic components inhibit angiotensin II‐induced cell‐cycle progression and migration: Involvement of cell‐cycle inhibitor p27^Kip1 and mitogen‐activated protein kinase

Garlic components inhibit angiotensin II‐induced cell‐cycle progression and migration: Involvement of cell‐cycle inhibitor p27^Kip1 and mitogen‐activated protein kinase

Pungent products from garlic activate the sensory ion channel TRPA1

Evaluating the effects of dark chocolate formulated with micro‐encapsulated fermented garlic extract on cardio‐metabolic indices in hypertensive patients: A crossover, triple‐blind placebo‐controlled randomized clinical trial

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An In Vitro Study of Different Extracts and Fractions of Allium sativum (Garlic): Vascular Reactivity

Cited by 13 publications

References 20 publications

Garlic components inhibit angiotensin II‐induced cell‐cycle progression and migration: Involvement of cell‐cycle inhibitor p27Kip1 and mitogen‐activated protein kinase

Garlic components inhibit angiotensin II‐induced cell‐cycle progression and migration: Involvement of cell‐cycle inhibitor p27Kip1 and mitogen‐activated protein kinase

Pungent products from garlic activate the sensory ion channel TRPA1

Evaluating the effects of dark chocolate formulated with micro‐encapsulated fermented garlic extract on cardio‐metabolic indices in hypertensive patients: A crossover, triple‐blind placebo‐controlled randomized clinical trial

Contact Info

Product

Resources

About

Garlic components inhibit angiotensin II‐induced cell‐cycle progression and migration: Involvement of cell‐cycle inhibitor p27^Kip1 and mitogen‐activated protein kinase

Garlic components inhibit angiotensin II‐induced cell‐cycle progression and migration: Involvement of cell‐cycle inhibitor p27^Kip1 and mitogen‐activated protein kinase