An in vitro study of electroporation of leukemia and cervical cancer cells

Ramachandran, Raja Prabu; Madhivanan, S; Sundararajan, Raji; Lin, Cindy; Kannan, Sankaranarayanan

doi:10.1533/9781908818294.161

Cited by 7 publications

(5 citation statements)

References 26 publications

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“…Previous reports have shown that the effectiveness of curcumin could be increased in human breast cancer MCF-7 cells and human leukemia HL-10 cells when electroporation was applied. 31 , 32 However, such intense PEF with invasive electrodes have been reported to cause unwanted side effects. Therefore, research for finding adequate approaches that enable low concentration of curcumin to achieve maximum effect with minimum side effect is of vital importance.…”

Section: Discussionmentioning

confidence: 99%

“… 17 , 37 , 38 Furthermore, the enhanced cytotoxic efficacy of curcumin by non-invasive PEF demonstrated comparable result to the application of standard PEF on conventional drugs or curcumin. 31 , 39 , 40 Most importantly, the approach performed in this study is a non-invasive, gentle, and long-term continuous treatment using contactless electrodes. In view of the modifiability of the electric field to focus on a specific location and the non-cytotoxicity of the combination treatment in HEK293 cells, this approach presents a means to achieve a cancer-specific effect in a safe manner.…”

Section: Discussionmentioning

confidence: 99%

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Enhanced anticancer effects of low-dose curcumin with non-invasive pulsed electric field on PANC-1 cells

Lin

Hsieh

et al. 2018

OTT

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BackgroundPulsed electric field (PEF) has been considered as a cell permeability enhancing agent for cancer treatment. Nevertheless, application of PEF for conventional electrochemo-therapy is usually at high intensity, and contact or even invasive electrodes are typically used, which may cause unwanted side effects. In this study, a non-invasive way of applying low intensity, non-contact PEF was adopted to study its combination effect with herb, curcumin, against pancreatic cancer cells and the mechanism involved.MethodsThe pancreatic cancer PANC-1 cells were treated with curcumin and PEF alone or in combination, and MTT assay was used to determine the viability of PANC-1 cells. Apoptosis and uptake of curcumin were analyzed by microscopy and flow cytometry. Western blot was further performed to evaluate the expression of apoptotic proteins.ResultsOur results demonstrated that PEF synergized with curcumin to inhibit the proliferation of PANC-1 cells in a field strength- and dose-dependent manner and caused apoptotic death of PANC-1 cells. The apoptotic induction of combination treatment was characterized by an increase in Bax/Bcl-2 ratio, and cleavage of caspase-8, -9, and -3. Moreover, the increase of curcumin uptake via electro-endocytosis was clearly observed in the cells following the exposure of PEF.ConclusionWe show for the first time that a non-contact approach using low intensity electric field in a pulsed waveform could enhance the anticancer effect of low-dose curcumin on PANC-1 cells through triggering both extrinsic and intrinsic pathways. The findings highlight the potential of this alternative treatment, non-invasive electric field and curcumin, to increase therapeutic efficacy with minimum cytotoxicity and side effects, which may provide a new aspect of cancer treatment in combination of PEF and other anticancer agents.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Enhanced anticancer effects of low-dose curcumin with non-invasive pulsed electric field on PANC-1 cells

Lin

Hsieh

et al. 2018

OTT

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“…THP-1 is a spontaneously immortalized monocyte-like cell line derived from the peripheral blood of a childhood case of acute monocytic leukemia (M5 subtype) . HL-60 is a human promyelocytic leukemia cell line with a p53-null mutation . Compound 48c demonstrated antiproliferative activity against these cell lines with IC 50 values in the 2–5 μM range (Figure A).…”

Section: Resultsmentioning

confidence: 99%

“…61 HL-60 is a human promyelocytic leukemia cell line with a p53-null mutation. 62 Compound 48c demonstrated antiproliferative activity against these cell lines with IC 50 values in the 2−5 μM range (Figure 8A). Like MOLM-14 cells, MOLM-13 AML cells have one wild-type FLT3 and one FLT3-ITD allele.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Design, Synthesis, and Biological Evaluation of Quinazolin-4-one-Based Hydroxamic Acids as Dual PI3K/HDAC Inhibitors

et al. 2020

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A series of quinazolin-4-one based hydroxamic acids was rationally designed and synthesized as novel dual PI3K/HDAC inhibitors by incorporating an HDAC pharmacophore into a PI3K inhibitor (Idelalisib) via an optimized linker. Several of these dual inhibitors were highly potent (IC 50 < 10 nM) and selective against PI3Kγ, δ and HDAC6 enzymes and exhibited good antiproliferative activity against multiple cancer cell lines. The lead compound 48c, induced necrosis in several mutant and FLT3-resistant AML cell lines and primary blasts from AML patients, while showing no cytotoxicity against normal PBMCs, NIH3T3, and HEK293 cells. Target engagement of PI3Kδ and HDAC6 by 48c was demonstrated in MV411 cells using the cellular thermal shift assay (CETSA). Compound 48c showed good pharmacokinetics properties in mice via intraperitoneal (ip) administration and provides a means to examine the biological effects of inhibiting these two important enzymes with a single molecule, either in vitro or in vivo.

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“…Researchers had demonstrated pulse electric eld synergized with low dose curcumin to inhibit proliferation of PANC-1, pancreatic cancer cells proliferation of PANC-1 cells in a eld strength-and dose-dependent manner and caused apoptotic death of PANC-1 cells [11]. Researchers have shown that electroporation can enhance the effectiveness of curcumin in MCF-7 cells and HL-10 cells [37,38]. Reports noted undesired side effect with intense PEF with invasive electrodes and work on novel approaches that enable low concentration of curcumin to achieve cytotoxicity is essential.…”

Section: Discussionmentioning

confidence: 99%

Combinatorial Anticancer Effects of The Non-Invasive High Voltage micro-second Pulse Electric Field with Low-Dose Curcumin on A549 Cells

KUMAR,

Pai,

Singh

et al. 2023

Preprint

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Purpose Novel, alternative and combinatorial approaches to combat cancers with minimal side effects is the need of the hour, owing to significant therapy-associated adverse effects. Natural molecule curcumin has shown to have significant anticancer action in lung cancers, a major cause of mortality world over. However, usage of curcumin is limited by its poor bioavailability. High voltage microsecond pulse electric field (HV-µsPEF) therapy is an emerging alternative option in cancer therapy which can be used as an effective adjuvant anti-cancer therapy. In the current investigation, we assessed the combinatorial effect of HV-µsPEF with low-dose curcumin on A549 lung cancer cell line with the main objective of minimization of the curcumin dose required for its anti-cancer action. Method HV-µsPEF was generated using a previously reported pulse-generator which was combined with low doses of curcumin to assess effects on A549 cell line. Phase-contrast microscopy based cellular morphology analysis, flow-cytometry based analyses to assess the mode of cell death, uptake of curcumin, assessment of reactive oxygen species and that of mitochondrial membrane potential (MMP) loss were carried out. Results Our work demonstrates a highly synergistic cell death induction in A549 cells as assessed by phase contrast microscopy and flow-cytometry based analysis due to the increased uptake of curcumin by cancer cells in the presence of HV-µsPEF. This led to increased ROS production and loss of MMP in cancer cells with the combinatorial treatment. Conclusion This study shows promising anti-cancer effects in the usage of low doses of curcumin, a natural molecule, with HV-µsPEF. Combined treatment of HV-µsPEF with curcumin in A549 cells increased the uptake of curcumin in the cells leading to effective induction of cell death via increased ROS production and MMP loss effectively minimizing the requirement of high doses of curcumin which are practically not achievable.

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An in vitro study of electroporation of leukemia and cervical cancer cells

Cited by 7 publications

References 26 publications

Enhanced anticancer effects of low-dose curcumin with non-invasive pulsed electric field on PANC-1 cells

Enhanced anticancer effects of low-dose curcumin with non-invasive pulsed electric field on PANC-1 cells

Design, Synthesis, and Biological Evaluation of Quinazolin-4-one-Based Hydroxamic Acids as Dual PI3K/HDAC Inhibitors

Combinatorial Anticancer Effects of The Non-Invasive High Voltage micro-second Pulse Electric Field with Low-Dose Curcumin on A549 Cells

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