An in vitro Technique for Measuring Cell Adhesion to Rigid Materials

Fletcher, Ronald D.; Schneider, Galen B.; Labant, MaryAnn; Albertson, J.N.

doi:10.1177/00220345790580072001

Cited by 8 publications

(7 citation statements)

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“…Previous studies of cell adhesion to metal implants have concentrated on assessing proliferation (Ciapetti et al, 1993;Grill et al, 1997) or adhesion strength (Dmytryk et al, 1990;Fletcher et al, 1979;Mustafa et al, 2001;Richards, 1996) on a fabricated implant. This research has been limited mainly to titanium surfaces, although some studies have investigated other metals (Iijima, 1989;Jansen et al, 1985;Nikolaychik, V et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

An in vitro model to evaluate cell adhesion to metals used in implantation shows significant differences between palladium and gold or platinum

Turner

Armitage

Butler

et al. 2004

Cell Biology International

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Adhesion of tissue cells to metallic implants is a major factor that is important for proper tissue integration. Adhesion of Swiss mouse 3T3 fibroblasts to gold, platinum and palladium surfaces was investigated. Immunofluorescence staining for the integrin subunits alphav and beta1 and the focal contact protein vinculin revealed that cells growing on gold and platinum expressed many focal contacts. In contrast, cells on palladium surfaces had reduced numbers of focal contacts shown by vinculin staining and failed to demonstrate expression of alphav and beta1 in focal contacts. Spread cell area was also significantly reduced on palladium than on other surfaces suggesting that cells on palladium were more weakly attached. This may be due to either a different molecular composition of focal contacts in cells grown on palladium surfaces or unusual microstructural properties of the palladium surface. This model is useful to evaluate adhesion of cells to different metal surfaces.

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Section: Introductionmentioning

confidence: 99%

An in vitro model to evaluate cell adhesion to metals used in implantation shows significant differences between palladium and gold or platinum

Turner

Armitage

Butler

et al. 2004

Cell Biology International

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“…It was also shown that secondary cultures were less easily detached from the sites where cells had previously been detached. Fletcher et al (1978) measured the degree of cell adhesion to dental implants by measuring the pressure from a small aperture which detached cells from the centre of a culture. Bell (1978) found that the force required to separate two cells or a cell from a surface was the same order of magnitude as the forces required to pull integral membrane proteins out of the cell membrane.…”

Section: Introductionmentioning

confidence: 99%

Microjet impingement followed by scanning electron microscopy as a qualitative technique to compare cellular adhesion to various biomaterials

Richards

Gwynn

et al. 1995

Cell Biology International

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Adhesion of cells to biomaterial surfaces is one of the major factors which mediates their biocompatibility. Quantitative or qualitative cell adhesion measurements would be useful for screening new implant materials. Microjet impingement has been evaluated by scanning electron microscopy, to determine to what extent it measures cell adhesion. The shear forces of the impingement, on the materials tested here, are seen to be greater than the cohesive strength of the cells in the impinged area, causing their rupture. The cell bodies are removed during impingement, leaving the sites of adhesion and other cellular material behind. Thus the method is shown not to provide quantification of cell adhesion forces for the metals and culture plastic tested. It is suggested that with highly adherent biomaterials, the distribution and patterns of these adhesion sites could be used for qualitative comparisons for screening of implant surfaces.

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“…At the conclusion of each time span, the masking tape seal from the outside of the hole was removed and the petri dish was placed with the aperture precisely over the debonding apparatus. The air pressure (cm HgO) to debond the various samples from the cell monolayer was measured and recorded on a strip chart recorder (Fletcher et al 1979).…”

Section: Methodsmentioning

confidence: 99%

“…Over a period of time, however, the epithelial cell attachment is broken rendering the implant useless (Smithloff & Fritz 1976). In an effort to ascertain which materials might avoid this rejection, gingival cell adhesions in vitro to various rigid materials (copper, glass, gold, polystyrene, silver, titanium, vitallium and vitreous carbon) were measured using the blister test (Fletcher et al 1979). In this study, titanium, polystyrene, glass and vitallium provided the best substratum for adhesion.…”

Section: Introductionmentioning

confidence: 99%

Quantitation of in vitro cell adhesion to cementum, dentin and enamel

Brastins

Labant

Fletcher

1980

J of Periodontal Research

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The William's blister test for adhesion was used to determine the strength of epithelial and fibroblast cell attachments to cementum, dentin and enamel. Differences of air pressure required (cm H2O) to debond the samples from a cell monolayer were measured and recorded on a strip chart recorder (Fletcher et al. 1977). Gingival and skin‐muscle cells detached at similar pressures from cementum, dentin and enamel. Higher pressures (of debond) were required with cells grown on a polystyrene surface.

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An in vitro Technique for Measuring Cell Adhesion to Rigid Materials

Cited by 8 publications

References 0 publications

An in vitro model to evaluate cell adhesion to metals used in implantation shows significant differences between palladium and gold or platinum

An in vitro model to evaluate cell adhesion to metals used in implantation shows significant differences between palladium and gold or platinum

Microjet impingement followed by scanning electron microscopy as a qualitative technique to compare cellular adhesion to various biomaterials

Quantitation of in vitro cell adhesion to cementum, dentin and enamel

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