An in vivo model of beta-adrenoceptor desensitization

Chong, B. T.Y.; Agrawal, Devendra K.; Romero, F.A.; Townley, Robert G.

doi:10.1016/s1056-8719(98)00046-x

Cited by 9 publications

(6 citation statements)

References 24 publications

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“…Previous studies have also shown a tolerance to shortacting b 2 -agonists in human 10 and animal 11 models. Investigators using animal models have used b 2 -agonists 11 or IL-1b 12,13 in vivo to study b 2 -AR tolerance. Although exposure of airway smooth muscle to IL-1b evokes b 2 -AR desensitization, the exact mechanism by which this occurs might not be identical to that which occurs in asthmatic patients.…”

Section: Discussionmentioning

confidence: 99%

Steroids completely reverse albuterol-induced β2-adrenergic receptor tolerance in human small airways

Cooper

Panettieri

2008

Journal of Allergy and Clinical Immunology

101

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Section: Discussionmentioning

confidence: 99%

Steroids completely reverse albuterol-induced β2-adrenergic receptor tolerance in human small airways

Cooper

Panettieri

2008

Journal of Allergy and Clinical Immunology

101

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“…Paradoxically, it seems that increased doses of β 2 AR-agonists (racemic form) may lose protective efficacy with time, and lead to a worsening of the condition [58,91]. (RS)-salbutamol intensifies bronchoconstrictor responses of asthmatic airways, and promotes the activation of human eosinophils in vitro [9,87].…”

Section: Pro-inflammatory or Anti-inflammatory Rolementioning

confidence: 99%

The Role of β2-Adrenergic Receptors in Inflammation and Allergy

Šitkauskienė¹,

Sakalauskas²

2005

CDTIA

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Essential role of beta(2)-adrenoreceptor (beta(2)AR) in airway relaxation is well established. Nevertheless, beta(2)AR seems playing an actual role in allergy and inflammation. Interaction between beta(2)AR and proinflamatory cytokines in airway smooth muscle has been revealed. Being located on proinflamatory cells, beta(2)ARs may influence function of these cells in vivo. It was clear established, that stimulation of beta(2)AR inhibits release of proinflamatory mediators from mast cells, influences T-cell growth and function, eosinophil survival and function, including GM-CSF- or PAF-induced degranulation. Stimulation of beta(2)ARs, located on alveolar macrophages and epithelial cells, has ambiguity influence on their regulation and function, including phagocytosis and mediator secretion, in vivo. Vascular responses, resulting in inhibition of plasma exudation were confirmed, but modulation of sensory nerves via beta(2)AR is not certain yet. beta(2)AR-agonists are effective in treatment of immediate allergic reactions, but desensitisation of beta(2)ARs on inflammatory cells may result in paradoxical effects, especially in asthma. In summary, it is clear that beta(2)ARs may play an anti-inflammatory role in vitro. Unfortunately, in vitro data have shown limited applicability in vivo; therefore further research in this field is required.

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“…In 1997, for the first time using UWBP, a study on airway hyperresponsiveness (AHR) using aerosolized methacholine challenge in conscious, spontaneously breathing mice, revealed a good correlation between Penh and readouts such as lung resistance obtained from an invasive method (Hamelmann et al, 1997). Following this pioneering study, a few more investigations confirmed good correlations between Penh and gold-standard functional parameters obtained from invasive lung function measurements (Chong et al, 1998;Berry et al, 1999;Finotto et al, 2001;Donaldson et al, 2002;Kumar et al, 2004). However, further experiments have raised serious uncertainties and questions such as: what Penh as a dimensionless index really measures and to what extent it can be related to lung mechanics (Lundblad et al, 2002;Mitzner and Tankersley, 2003;Adler et al, 2004;Bates et al, 2004;Sly et al, 2005).…”

Section: Non-imaging-based Techniques 2211 Unrestrained Whole-body Pl...mentioning

confidence: 95%

“…This technique provides parameters such as tidal volume and respiratory frequency (Glaab et al, 2007;Vanoirbeek et al, 2010;Bates, 2017). UWBP has been employed by many researchers for longitudinal measurement of lung functions in spontaneously breathing animals, especially by reporting a parameter called enhanced pause (Penh) (Hamelmann et al, 1997;Chong et al, 1998;Finotto et al, 2001;Donaldson et al, 2002). Penh is a dimensionless parameter used for the evaluation of changes in the shape of the airflow entering and leaving a whole-body plethysmograph (Bates, 2017).…”

Section: Non-imaging-based Techniques 2211 Unrestrained Whole-body Pl...mentioning

confidence: 99%

Lung function measurements in preclinical research: What has been done and where is it headed?

2023

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Due to the close interaction of lung morphology and functions, repeatable measurements of pulmonary function during longitudinal studies on lung pathophysiology and treatment efficacy have been a great area of interest for lung researchers. Spirometry, as a simple and quick procedure that depends on the maximal inspiration of the patient, is the most common lung function test in clinics that measures lung volumes against time. Similarly, in the preclinical area, plethysmography techniques offer lung functional parameters related to lung volumes. In the past few decades, many innovative techniques have been introduced for in vivo lung function measurements, while each one of these techniques has their own advantages and disadvantages. Before each experiment, depending on the sensitivity of the required pulmonary functional parameters, it should be decided whether an invasive or non-invasive approach is desired. On one hand, invasive techniques offer sensitive and specific readouts related to lung mechanics in anesthetized and tracheotomized animals at endpoints. On the other hand, non-invasive techniques allow repeatable lung function measurements in conscious, free-breathing animals with readouts related to the lung volumes. The biggest disadvantage of these standard techniques for lung function measurements is considering the lung as a single unit and providing only global readouts. However, recent advances in lung imaging modalities such as x-ray computed tomography and magnetic resonance imaging opened new doors toward obtaining both anatomical and functional information from the same scan session, without the requirement for any extra pulmonary functional measurements, in more regional and non-invasive manners. Consequently, a new field of study called pulmonary functional imaging was born which focuses on introducing new techniques for regional quantification of lung function non-invasively using imaging-based techniques. This narrative review provides first an overview of both invasive and non-invasive conventional methods for lung function measurements, mostly focused on small animals for preclinical research, including discussions about their advantages and disadvantages. Then, we focus on those newly developed, non-invasive, imaging-based techniques that can provide either global or regional lung functional readouts at multiple time-points.

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An in vivo model of beta-adrenoceptor desensitization

Cited by 9 publications

References 24 publications

Steroids completely reverse albuterol-induced β2-adrenergic receptor tolerance in human small airways

Steroids completely reverse albuterol-induced β2-adrenergic receptor tolerance in human small airways

The Role of β2-Adrenergic Receptors in Inflammation and Allergy

Lung function measurements in preclinical research: What has been done and where is it headed?

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An in vivo model of beta-adrenoceptor desensitization

Cited by 9 publications

References 24 publications

Steroids completely reverse albuterol-induced β2-adrenergic receptor tolerance in human small airways

Steroids completely reverse albuterol-induced β2-adrenergic receptor tolerance in human small airways

The Role of &#946;2-Adrenergic Receptors in Inflammation and Allergy

Lung function measurements in preclinical research: What has been done and where is it headed?

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The Role of β2-Adrenergic Receptors in Inflammation and Allergy