An in vivo model of apoptosis: linking cell behaviours and caspase substrates in embryos lacking DIAP1

Chandraratna, Dhianjali; Lawrence, Nicola; Welchman, David P.; Sanson, Bénédicte

doi:10.1242/jcs.03472

Cited by 11 publications

(9 citation statements)

References 68 publications

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“…DIAP1 is in a complex with Rac1 and Profilin and enables border cell motility apparently by promoting actin turnover (Geisbrecht and Montell 2004). Further, in the embryo, DIAP1 loss leads to Dlg cleavage and cellular rounding and dispersal (Chandraratna et al 2007). Too much DIAP1 also appears to be deleterious to movement, because targeted overexpression of DIAP1 specifically in border cells slows their migration (data not shown).…”

Section: Discussionmentioning

confidence: 96%

Basolateral Junctions Utilize Warts Signaling to Control Epithelial-Mesenchymal Transition and Proliferation Crucial For Migration and Invasion of Drosophila Ovarian Epithelial Cells

Zhao¹,

Szafrański²,

Hall³

et al. 2008

Genetics

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Fasciclin2 (Fas2) and Discslarge (Dlg) localize to the basolateral junction (BLJ) of Drosophila follicle epithelial cells and inhibit their proliferation and invasion. To identify a BLJ signaling pathway we completed a genomewide screen for mutants that enhance dlg tumorigenesis. We identified two genes that encode known BLJ scaffolding proteins, lethal giant larvae (lgl) and scribble (scrib), and several not previously associated with BLJ function, including warts (wts) and roughened eye (roe), which encode a serine-threonine kinase and a transcription factor, respectively. Like scrib, wts and roe also enhance Fas2 and lgl tumorigenesis. Further, scrib, wts, and roe block border cell migration, and cause noninvasive tumors that resemble dlg partial loss of function, suggesting that the BLJ utilizes Wts signaling to repress EMT and proliferation, but not motility. Apicolateral junction proteins Fat (Ft), Expanded (Ex), and Merlin (Mer) either are not involved in these processes, or have highly spatio-temporally restricted roles, diminishing their significance as upstream inputs to Wts in follicle cells. This is further indicated in that Wts targets, CyclinE and DIAP1, are elevated in Fas2, dlg, lgl, wts, and roe cells, but not Fat, ex, or mer cells. Thus, the BLJ appears to regulate epithelial polarity and dynamics not only as a localized scaffold, but also by communicating signals to the nucleus. Wts may be regulated by distinct junction inputs depending on developmental context.

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Section: Discussionmentioning

confidence: 96%

Basolateral Junctions Utilize Warts Signaling to Control Epithelial-Mesenchymal Transition and Proliferation Crucial For Migration and Invasion of Drosophila Ovarian Epithelial Cells

Zhao¹,

Szafrański²,

Hall³

et al. 2008

Genetics

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“…DIAP1 (Figure 1), referred as a “gatekeeper of death” [3], is essential to ensure cell survival, neutralization of DIAP1 being necessary and sufficient to trigger apoptosis [33,40,44]. Loss-of-function mutations in DIAP1encoding gene ( thread ) lead to early embryonic death with a massive caspase-dependent apoptosis [33,51,52]. Diap1 -deficiency-induced cell death is rescued by the inactivation of DRONC or DARK [40].…”

Section: Regulation Of Apoptosis By Iaps In Invertebratesmentioning

confidence: 99%

Regulation of Apoptosis by Inhibitors of Apoptosis (IAPs)

Berthelet

Dubrez

2013

Cells

133

106

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Inhibitors of Apoptosis (IAPs) are a family of proteins with various biological functions including regulation of innate immunity and inflammation, cell proliferation, cell migration and apoptosis. They are characterized by the presence of at least one N-terminal baculoviral IAP repeat (BIR) domain involved in protein-protein interaction. Most of them also contain a C-terminal RING domain conferring an E3-ubiquitin ligase activity. In drosophila, IAPs are essential to ensure cell survival, preventing the uncontrolled activation of the apoptotic protease caspases. In mammals, IAPs can also regulate apoptosis through controlling caspase activity and caspase-activating platform formation. Mammalian IAPs, mainly X-linked IAP (XIAP) and cellular IAPs (cIAPs) appeared to be important determinants of the response of cells to endogenous or exogenous cellular injuries, able to convert the survival signal into a cell death-inducing signal. This review highlights the role of IAP in regulating apoptosis in Drosophila and Mammals.

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“…28, 29 During the later apoptotic shrinking phase, AJ components are lost in a caspase-dependent manner, which is in agreement with studies, indicating that AJ components are bona fide caspase substrates. 25, 30, 31, 32 …”

Section: Discussionmentioning

confidence: 99%

JAK/STAT signaling is necessary for cell monosis prior to epithelial cell apoptotic extrusion

et al. 2017

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Epithelial cell extrusion is crucial for proper development and tissue homeostasis. High-resolution 3D reconstruction and 4D imaging, combined with genetic analyis, have allowed us to reveal the highly-sterotyped morphogenetic events controlled by JAK/STAT signaling in a developmentally-programmed case of epithelial cell extrusion. Specialized somatic cells, Polar Cells (PCs), are produced in excess and then undergo apoptotic elimination from the follicular epithelium in the Drosophila ovary. We show that supernumerary PCs are first systematically enveloped by PC neighbors on all sides, first laterally, then apically in conjunction with highly-reinforced adherens junctions, and finally basally. The PC to be removed thus loses all contact with follicle cells, germline cells and the basement membrane in a process we have called cell ‘monosis’, for ‘isolation’ in Greek. PC monosis takes several hours, and always precedes, and is independent of, activation of apoptosis. JAK/STAT signaling is necessary within the surrounding follicular epithelium for PC monosis. Minutes after monosis is complete, PC apoptotic corpses are formed and extruded laterally within the epithelium, in contrast to the apical and basal extrusions described to date. These apoptotic corpses are engulfed and eliminated by surrounding follicle cells, which are thus acting as non-professional phagocytes. This study therefore shows the non cell-autonomous impact of an epithelium, via JAK/STAT signaling activation, on cell morphogenesis events leading to apoptotic extrusion. It is likely that the use of high-resolution 3D and 4D imaging, which allows for better spatio-temporal understanding of morphogenetic events, will reveal that cell monosis and lateral extrusion within an epithelium are pertinent for other cases of epithelial cell extrusion as well.

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An in vivo model of apoptosis: linking cell behaviours and caspase substrates in embryos lacking DIAP1

Abstract: SummaryAn in vivo model of apoptosis: linking cell behaviours and caspase substrates in embryos lacking DIAP1

Cited by 11 publications

References 68 publications

Basolateral Junctions Utilize Warts Signaling to Control Epithelial-Mesenchymal Transition and Proliferation Crucial For Migration and Invasion of Drosophila Ovarian Epithelial Cells

Basolateral Junctions Utilize Warts Signaling to Control Epithelial-Mesenchymal Transition and Proliferation Crucial For Migration and Invasion of Drosophila Ovarian Epithelial Cells

Regulation of Apoptosis by Inhibitors of Apoptosis (IAPs)

JAK/STAT signaling is necessary for cell monosis prior to epithelial cell apoptotic extrusion

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