An In Vivo Perspective of the Role(s) of the Alternative Oxidase Pathway

Del‐Saz, Néstor Fernández; Ribas‐Carbó, Miquel; McDonald, Allison E.; Lambers, Hans; Fernie, Alisdair R.; Florez‐Sarasa, Igor

doi:10.1016/j.tplants.2017.11.006

Cited by 138 publications

(138 citation statements)

References 117 publications

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“…Involvement of AOX in stress responses was also proposed in other photosynthetic organisms (Van Aken et al ., ; Feng et al ., ; Vanlerberghe, ; Del‐Saz et al ., ). Likewise, AOX‐C5 showed higher sensitivity to stress (Fig.…”

Section: Resultsmentioning

confidence: 97%

“…In transgenic tobacco cells silencing of AOX expression resulted in accumulation of ROS, while overexpression of AOX decreased ROS levels (Maxwell et al ., ), and AOX was shown to limit superoxide production (Purvis, ). Since these first reports, numerous other groups have demonstrated that AOX may enhance an organism's ability to resist a variety of stressors by reducing the level of oxidative stress (Rasmusson et al ., ; Van Aken et al ., ; Del‐Saz et al ., ). AOX was shown to be involved in the defense against drought in N. tabacum leaves due to its ability to reduce oxidative damage (Dahal & Vanlerberghe, ).…”

Section: Introductionmentioning

confidence: 97%

“…AOX was shown to be involved in the defense against drought in N. tabacum leaves due to its ability to reduce oxidative damage (Dahal & Vanlerberghe, ). Conditions reported to modulate AOX expression or activity include chilling, drought, light stress, high salt, nutrient limitation, ozone, metal toxicity and heat stress (see Feng et al ., ; Del‐Saz et al ., , and references therein). Several signaling molecules have been reported to regulate AOX under stress, including calcium, nitric oxide (NO), abscisic acid, salicylic acid, ethylene, H 2 O 2 , superoxide, cyanide and citrate (Feng et al ., ; Vanlerberghe, ).…”

Section: Introductionmentioning

confidence: 99%

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Downregulation of mitochondrial alternative oxidase affects chloroplast function, redox status and stress response in a marine diatom

et al. 2018

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Diatom dominance in contemporary aquatic environments indicates that they have developed unique and effective mechanisms to cope with the rapid and considerable fluctuations that characterize these environments. In view of their evolutionary history from a secondary endosymbiosis, inter-organellar regulation of biochemical activities may be of particular relevance. Diatom mitochondrial alternative oxidase (AOX) is believed to play a significant role in supplying chloroplasts with ATP produced in the mitochondria. Using the model diatom Phaeodactylum tricornutum we generated AOX knockdown lines, and followed sensitivity to stressors, photosynthesis and transcriptome and metabolome profiles of wild-type and knockdown lines. We show here that expression of the AOX gene is upregulated by various stresses including H O , heat, high light illumination, and iron or nitrogen limitation. AOX knockdown results in hypersensitivity to stress. Knockdown lines also show significantly reduced photosynthetic rates and their chloroplasts are more oxidized. Comparisons of transcriptome and metabolome profiles suggest a strong impact of AOX activity on gene expression, which is carried through to the level of the metabolome. Our data provide evidence for the involvement of mitochondrial AOX in processes central to the cell biology of diatoms, revealing that cross-talk between mitochondria and chloroplasts is crucial for maintaining sensitivity to changing environments.

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Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Downregulation of mitochondrial alternative oxidase affects chloroplast function, redox status and stress response in a marine diatom

et al. 2018

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“…As the biomass of the photosynthetically active seedlings remained constant, energy dissipation was likely to be important, as indicated by their high AOX capacity. While oxygen isotope fractionation would be needed to establish the actual partition of electron in the alternative pathway in vivo , the AOX capacity is an indicator of the potential electron flux in the pathway (Del‐Saz et al ., ). AOX plays an important role in photosynthetic adaptation to high light (Yoshida et al ., ; Dinakar et al ., ) but also to macronutrients limited conditions (Sieger et al ., ; Escobar et al ., ; Vijayraghavan and Soole, ).…”

Section: Discussionmentioning

confidence: 97%

Arabidopsis seedlings display a remarkable resilience under severe mineral starvation using their metabolic plasticity to remain self‐sufficient for weeks

et al. 2019

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During the life cycle of plants, seedlings are considered vulnerable because they are at the interface between the highly stress tolerant seed embryos and the established plant, and must develop rapidly, often in a challenging environment, with limited access to nutrients and light. Using a simple experimental system, whereby the seedling stage of Arabidopsis is considerably prolonged by nutrient starvation, we analysed the physiology and metabolism of seedlings maintained in such conditions up to 4 weeks. Although development was arrested at the cotyledon stage, there was no sign of senescence and seedlings remained viable for weeks, yielding normal plants after transplantation. Photosynthetic activity compensated for respiratory carbon losses, and energy dissipation by photorespiration and alternative oxidase appeared important. Photosynthates were essentially stored as organic acids, while the pool of free amino acids remained stable. Seedlings lost the capacity to store lipids in cytosolic lipid droplets, but developed large plastoglobuli. Arabidopsis seedlings arrested in their development because of mineral starvation displayed therefore a remarkable resilience, using their metabolic and physiological plasticity to maintain a steady state for weeks, allowing resumption of development when favourable conditions ensue.

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“…One of the most effective AOX activators at the low level of reduced ubiquinone is pyruvate. The availability of AOX substrates, such as a reduced fraction of the ubiquinone pool and oxygen pool, also affects the AOX activity (Del-Saz et al 2018).…”

Section: mentioning

confidence: 99%

Application of abscisic acid prevents leaf senescence at decreased irradiance

Kupis¹,

Tomal²,

Niewiadomska³

2019

Biol. plant.

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Several lines of evidence suggest that plant hormone abscisic acid (ABA) and the activity of alternative respiratory pathway are somehow interrelated. In this paper, we tested how an application of ABA affects the alternative oxidase (AOX) pathway and leaf senescence in Arabidopsis thaliana (L.) Heynh. Different effects of ABA application on the AOX activity were observed under high irradiance (HI) and low irradiance (LI). The expression of AOX1D gene was stimulated, whereas the expression of senescence marker gene WRKY53 was decreased due to ABA treatment. We also noticed that changes of AOX genes AOX1A and AOX1D, as well as AOX regulatory gene ANAC017, were rather inversely correlated with irradiance-dependent changes in AOX activity. The expression of AOX1D and WRKY53 was diminished due to the application of H 2 O 2 under LI. Our results are indicative that in some conditions an exogenous application of ABA may suppress leaf senescence. This action does not go via the induction of AOX activity, despite stimulation of AOX genes.

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An In Vivo Perspective of the Role(s) of the Alternative Oxidase Pathway

Cited by 138 publications

References 117 publications

Downregulation of mitochondrial alternative oxidase affects chloroplast function, redox status and stress response in a marine diatom

Downregulation of mitochondrial alternative oxidase affects chloroplast function, redox status and stress response in a marine diatom

Arabidopsis seedlings display a remarkable resilience under severe mineral starvation using their metabolic plasticity to remain self‐sufficient for weeks

Application of abscisic acid prevents leaf senescence at decreased irradiance

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