An in vivo RNAi screen identifies evolutionary conserved Drosophila fat storage regulators

Abstract

An in vivo RNAi screen identifies evolutionary conserved Drosophila fat storage regulators Dissertation zur Erlangung des mathematisch-naturwissenschaftlichen Doktorgrades "Doctor rerum naturalium" der Georg-August-Universität Göttingen im Promotionsprogramm "Grundprogramm-Biologie" der Georg-August University School of Science (GAUSS) vorgelegt von Jens Baumbach aus Munster Göttingen, den 27.03.2014

Regulation of Drosophila melanogaster body fat storage by store-operated calcium entry

“…Moreover, Akh/AkhR likely regulates lipolysis at transcription level via the cAMP-responsive transcription factor Cyclic-AMP response element binding protein B (dCREB2). Thus overexpression of dominant-negative form of dCREB2 in fat body tissue not only increased lipid content (Iijima et al, 2009), but also repressed the expression of lipase gene bmm and increased the expression of the lipid synthesis gene mdy (Baumbach, 2014). In fact, to meet energy demands, basally and partially induced lipolysis are also independently carried out by BMM (Grönke et al, 2007).…”

“…In order to overcome the experimental limitations that might be associated with the six days lasting thermal stress conditions, comparatively short-term Stim knockdown period in fat storage tissues was performed and shown to causes a long-term effect on fat accumulation, resulting in obese flies even 30 days after they passed the 34 hours thermal treatment (Baumbach, 2014;Borcherding, 2014). However, the exact phenotypes were not completely characterized, and their underlying mechanisms [1]; P{y[+t7.7] v[+t1.8]…”

“…Long-term knockdown of Stim by six days of RNAi expression was employed to study the underlying mechanisms of fat storage (Baumbach, 2014;Baumbach et al, 2014a). However, the long-term knockdown by six days of incubation at 29°C is stressful for flies (Sayeed and Benzer, 1996).…”

“…To minimize the interference of the high temperature incubation with fat storage, the incubation time was reduced from six days to a transient incubation period of 34-38 hours of the adult flies. Transient 29°C incubation treatment revealed that flies continued gaining fat and becoming obese even one month after the treatment (Baumbach, 2014;Borcherding, 2014). (Baumbach, 2014).…”

“…Transient 29°C incubation treatment revealed that flies continued gaining fat and becoming obese even one month after the treatment (Baumbach, 2014;Borcherding, 2014). (Baumbach, 2014). Since Stim is ubiquitously expressed and found in all tissues (Attrill et al, 2016), I specifically dissected abdomen fat body tissues from adult male flies for RT-qPCR analysis.…”