An Increase of 63kDa-Protein Present in the Cell Membranes of Staphylococcus aureus That Bears a Plasmid Mediating Inducible Resistance to Partial Macrolide and Streptogramin B Antibiotics.

Matsuoka, Masao; Janosi, Laszlo; Endou, Kikutarou; Saitoh, Shiori; Hashimoto, Hajime; Nakajima, Yoshinori

doi:10.1248/bpb.16.1288

Cited by 8 publications

(10 citation statements)

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“…The msrSA gene encodes for a cell-membrane protein that acts as an active erythromycin-efflux pump. This gene was first described in a Staphylococcus aureus isolate, but it was also found in Bacteroides strains [13,14].…”

Section: Introductionmentioning

confidence: 94%

The prevalence of antibiotic resistance genes in Bacteroides fragilis group strains isolated in different European countries

et al. 2013

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Section: Introductionmentioning

confidence: 94%

The prevalence of antibiotic resistance genes in Bacteroides fragilis group strains isolated in different European countries

et al. 2013

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“…The sequence of the N-terminal amino acid residues of a 63 kDa protein (MsrSA) that appeared in the membrane of PMS-resistant strains was identical to that of an MsrA polypeptide related to enhanced e¥ux of [ 14 C]EM [12]. Ribosomes from PMS-resistant strains showed a similar a¤nity for EM to those from the PMS-sensitive host strain NCTC8325, and no inactivation of EM by 8325(pEP2104) was observed [11].…”

Section: Introductionmentioning

confidence: 96%

“…We have shown that Staphylococcus aureus 8325(pEP2104) exhibits inducible resistance to PMS [11] (partial macrolide and streptogramin B) antibiotics (the 14-membered macrolides, erythromycin (EM), and oleandomycin (OL), and the 16-membered macrolide mycinamicin (MCM) and STG-B). The sequence of the N-terminal amino acid residues of a 63 kDa protein (MsrSA) that appeared in the membrane of PMS-resistant strains was identical to that of an MsrA polypeptide related to enhanced e¥ux of [ 14 C]EM [12].…”

Section: Introductionmentioning

confidence: 99%

Cloning and sequences of inducible and constitutive macrolide resistance genes in Staphylococcus aureus that correspond to an ABC transporter

Matsuoka

1999

FEMS Microbiology Letters

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A restriction map was made and the DNA sequence was determined for a plasmid, pMC38, derived from the inducible macrolide resistance plasmid pEP2104, that showed constitutive resistance to PMS antibiotics (partial macrolide and streptogramin B antibiotics). A 5.04 kb SalI-PstI fragment (fragment C) of pMC38, which encoded PMS resistance, was cloned into a shuttle vector, pRIT5, to yield pMR504. The transformant Staphylococcus aureus 4220 (pMR504) exhibited constitutive PMS resistance. Fragment C was subcloned to pUC19 in order to determine the DNA sequence. This sequence was consequently found to contain three open reading frames (ORF1^3), of which ORF3 corresponded to the 63 kDa membrane protein (MsrSA) that expressed PMS resistance. According to DNA sequence comparison of the control region of ORF3 in pMC38 and pEP2104, 44 nucleotides including RBS1 and the leader peptide (MTASMRLK) were deleted on plasmid pMC38. This suggests that the leader peptide is essential for the inducible expression of PMS resistance. ß

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“…The sequence of the N‐terminal amino acid residues of a 63 kDa protein (MsrSA) that appeared in the membrane of PMS‐resistant strains was identical to that of an MsrA polypeptide related to enhanced efflux of [ 14 C]EM [12]. Ribosomes from PMS‐resistant strains showed a similar affinity for EM to those from the PMS‐sensitive host strain NCTC8325, and no inactivation of EM by 8325(pEP2104) was observed [11].…”

Section: Introductionmentioning

confidence: 97%

“…We have shown that Staphylococcus aureus 8325(pEP2104) exhibits inducible resistance to PMS [11] (partial macrolide and streptogramin B) antibiotics (the 14‐membered macrolides, erythromycin (EM), and oleandomycin (OL), and the 16‐membered macrolide mycinamicin (MCM) and STG‐B). The sequence of the N‐terminal amino acid residues of a 63 kDa protein (MsrSA) that appeared in the membrane of PMS‐resistant strains was identical to that of an MsrA polypeptide related to enhanced efflux of [ 14 C]EM [12].…”

Section: Introductionmentioning

confidence: 99%

Cloning and sequences of inducible and constitutive macrolide resistance genes inStaphylococcus aureusthat correspond to an ABC transporter

Matsuoka

Janosi

Endou

et al. 1999

FEMS Microbiology Letters

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A restriction map was made and the DNA sequence was determined for a plasmid, pMC38, derived from the inducible macrolide resistance plasmid pEP2104, that showed constitutive resistance to PMS antibiotics (partial macrolide and streptogramin B antibiotics). A 5. 04 kb SalI-PstI fragment (fragment C) of pMC38, which encoded PMS resistance, was cloned into a shuttle vector, pRIT5, to yield pMR504. The transformant Staphylococcus aureus 4220 (pMR504) exhibited constitutive PMS resistance. Fragment C was subcloned to pUC19 in order to determine the DNA sequence. This sequence was consequently found to contain three open reading frames (ORF1-3), of which ORF3 corresponded to the 63 kDa membrane protein (MsrSA) that expressed PMS resistance. According to DNA sequence comparison of the control region of ORF3 in pMC38 and pEP2104, 44 nucleotides including RBS1 and the leader peptide (MTASMRLK) were deleted on plasmid pMC38. This suggests that the leader peptide is essential for the inducible expression of PMS resistance.

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An Increase of 63kDa-Protein Present in the Cell Membranes of Staphylococcus aureus That Bears a Plasmid Mediating Inducible Resistance to Partial Macrolide and Streptogramin B Antibiotics.

Cited by 8 publications

References 0 publications

The prevalence of antibiotic resistance genes in Bacteroides fragilis group strains isolated in different European countries

The prevalence of antibiotic resistance genes in Bacteroides fragilis group strains isolated in different European countries

Cloning and sequences of inducible and constitutive macrolide resistance genes in Staphylococcus aureus that correspond to an ABC transporter

Cloning and sequences of inducible and constitutive macrolide resistance genes inStaphylococcus aureusthat correspond to an ABC transporter

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