An increase of lycopene content in tomato fruit is associated with a novel Cyc-B allele isolated through TILLING technology

Silletti, Maria Francesca; Petrozza, Angelo; Stigliani, Adriana Lucia; Giorio, Giovanni; Cellini, Francesco; D’Ambrosio, Caterina; Carriero, Filomena

doi:10.1007/s11032-012-9824-6

Cited by 15 publications

(7 citation statements)

References 32 publications

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“…Additional mutants are known to affect β‐carotene biosynthesis in tomato fruits, such as old gold and Beta, which result respectively in an impairment or an enhancement, of the fruit‐specific CYC‐b cyclase (Ronen et al , ). An old gold allele shows increased fruit firmness, although its fruit ABA content has not been studied (Silletti et al , ). Beta alleles carry a poorly characterized chromosomal introgression from green‐fruited tomato species, which results in complex vegetative and fruit phenotypes (unpublished data).…”

Section: Discussionmentioning

confidence: 99%

Manipulation of β‐carotene levels in tomato fruits results in increased ABA content and extended shelf life

Diretto

Frusciante

Fabbri

et al. 2019

Plant Biotechnology Journal

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Summary Tomato fruit ripening is controlled by the hormone ethylene and by a group of transcription factors, acting upstream of ethylene. During ripening, the linear carotene lycopene accumulates at the expense of cyclic carotenoids. Fruit‐specific overexpression of LYCOPENE β‐CYCLASE (LCYb) resulted in increased β‐carotene (provitamin A) content. Unexpectedly, LCYb‐overexpressing fruits also exhibited a diverse array of ripening phenotypes, including delayed softening and extended shelf life. These phenotypes were accompanied, at the biochemical level, by an increase in abscisic acid (ABA) content, decreased ethylene production, increased density of cell wall material containing linear pectins with a low degree of methylation, and a thicker cuticle with a higher content of cutin monomers and triterpenoids. The levels of several primary metabolites and phenylpropanoid compounds were also altered in the transgenic fruits, which could be attributed to delayed fruit ripening and/or to ABA. Network correlation analysis and pharmacological experiments with the ABA biosynthesis inhibitor, abamine, indicated that altered ABA levels were a direct effect of the increased β‐carotene content and were in turn responsible for the extended shelf life phenotype. Thus, manipulation of β‐carotene levels results in an improvement not only of the nutritional value of tomato fruits, but also of their shelf life.

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Section: Discussionmentioning

confidence: 99%

Manipulation of β‐carotene levels in tomato fruits results in increased ABA content and extended shelf life

Diretto

Frusciante

Fabbri

et al. 2019

Plant Biotechnology Journal

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“…This observation confirms previous work by Barry and Pandey [30] identified this allele as responsible for the brown fruit color phenotype. Another gene that plays an important role in orange color formation and involved in improving carotenoid content in tomato fruit is CYC-B [33, 34]. This gene is suggested to play a major role in fruit pigmentation of the KNY2 inbred during the ripening stages.…”

Section: Resultsmentioning

confidence: 99%

Molecular breeding of a novel orange-brown tomato fruit with enhanced beta-carotene and chlorophyll accumulation

et al. 2017

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BackgroundTomatoes provide a significant dietary source of the carotenoids, lycopene and β-carotene. During ripening, carotenoid accumulation determines the fruit colors while chlorophyll degradation. These traits have been, and continue to be, a significant focus for plant breeding efforts. Previous work has found strong evidence for a relationship between CYC-B gene expression and the orange color of fleshy fruit. Other work has identified a point mutation in SGR that impedes chlorophyll degradation and causes brown flesh color to be retained in some tomato varieties.MethodsWe crossed two inbred lines, KNY2 (orange) and KNB1 (brown) and evaluated the relationship between these genes for their effect on fruit color. Phenotypes of F2 generation plants were analyzed and a novel ‘orange-brown’ fruit color was identified.ResultsWe confirm two SNPs, one in CYC-B and another in SGR gene sequence, associated with segregation of ‘orange-brown’ fruit color in F2 generation. The carotenoid and chlorophyll content of a fleshy fruit was assessed across the different phenotypes and showed a strong correlation with expression pattern of carotenoid biosynthesis genes and SGR function. The orange-brown fruit has high β-carotene and chlorophyll. Our results provide valuable information for breeders to develop tomato fruit of a novel color using molecular markers.Electronic supplementary materialThe online version of this article (doi:10.1186/s41065-016-0023-z) contains supplementary material, which is available to authorized users.

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“…With respect to the B locus, natural variation can be exploited to modulate levels of β‐carotene in ripe tomato fruit. Cultivated varieties with elevated β‐carotene and lycopene content are currently available on the market; however, further modulation of carotenoid content may be required when developing new biofortified foods (Hirschi, 2008; Silletti et al., 2013; Welch, 2002). In addition, plant genetic resources with assorted carotenoid contents can be used to test hypotheses about human adsorption and utilization and metabolism of nutrients in the food matrix (Cooperstone et al., 2015; Kopec et al., 2014).…”

Section: Discussionmentioning

confidence: 99%

Identification and assessment of alleles in the promoter of the Cyc‐B gene that modulate levels of β‐carotene in ripe tomato fruit

et al. 2021

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Novel diversity may be mined from databases and de novo sequencing, but functional characterization remains a limiting step to identifying new alleles. Classical breeding approaches augmented by marker‐assisted selection offer a means to rapidly assess the function of new variation in coding or regulatory regions to modulate traits. We used the Cyc‐B gene (B) of tomato (Solanum lycopersicum L.) for a proof of concept because of its role in the production of β‐carotene, a provitamin A carotenoid with importance to human nutrition. We measured carotenoid content in vintage and contemporary varieties and the profiles had a range of β‐carotene from 0.2 to 4.06 mg 100 g−1 fresh weight. We characterized variation in B from 84 sequences recovered from public databases and from an additional 29 high β‐carotene tomato, S. galapagense S. C. Darwin & Peralta, and S. cheesmaniae (L. Riley) Fosberg accessions. Thirteen unique haplotypes across 1600 bp of sequence 5′ to the first ATG were identified with 11 occurring in high β‐carotene accessions we sequenced, and additional haplotypes were identified in public data. Phylogenetic analysis suggested that the alleles in high β‐carotene varieties were derived from wild species. Association analysis suggested two single nucleotide polymorphisms (SNPs) as the most likely causes of high β‐carotene, presumably through their influence on transcription of B that is elevated in ripening fruit. A marker‐assisted backcross breeding scheme leveraging SNPs for background genome selection was used to rapidly develop germplasm resources containing different alleles of B in a uniform genetic background. Evaluation demonstrated that distinct promoter haplotypes function as different alleles that can be used to modulate the levels of β‐carotene in tomato.

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An increase of lycopene content in tomato fruit is associated with a novel Cyc-B allele isolated through TILLING technology

Cited by 15 publications

References 32 publications

Manipulation of β‐carotene levels in tomato fruits results in increased ABA content and extended shelf life

Manipulation of β‐carotene levels in tomato fruits results in increased ABA content and extended shelf life

Molecular breeding of a novel orange-brown tomato fruit with enhanced beta-carotene and chlorophyll accumulation

Identification and assessment of alleles in the promoter of the Cyc‐B gene that modulate levels of β‐carotene in ripe tomato fruit

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