An increased level of sperm abnormalities in mice with a partial deletion of the Y chromosome

Styrna, Józefa; Imai, Hirotami T.; Moriwaki, Kazuo

doi:10.1017/s0016672300029268

Cited by 80 publications

(46 citation statements)

References 6 publications

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“…Deletions in the MSYq region have emerged as the most common genetic cause for spermatogenic failures in the human population worldwide, resulting in oligo-or azoospermia (30). In mice, deficiency in MSYq transcripts deriving from mousespecific multicopy genes causes abnormalities in the sperm heads, and MSYq deletion mutants display a range of teratozoospermia and infertility phenotypes with severities corresponding to the extent of the deletion (23,25,26,(31)(32)(33). The increase in flat sperm heads is specific to MSYq deletions in mice, and even minor changes in the sperm head structure can affect sperm motility and thereby fertility (26,32,33).…”

Section: Discussionmentioning

confidence: 99%

Promoter ChIP-chip analysis in mouse testis reveals Y chromosome occupancy by HSF2

Åkerfelt

Henriksson

Laiho

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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The mammalian Y chromosome is essential for spermatogenesis, which is characterized by sperm cell differentiation and chromatin condensation for acquisition of correct shape of the sperm. Deletions of the male-specific region of the mouse Y chromosome long arm (MSYq), harboring multiple copies of a few genes, lead to sperm head defects and impaired fertility. Using chromatin immunoprecipitation on promoter microarray (ChIP-chip) on mouse testis, we found a striking in vivo MSYq occupancy by heat shock factor 2 (HSF2), a transcription factor involved in spermatogenesis. HSF2 was also found to regulate the transcription of MSYq resident genes, whose transcriptional regulation has been unknown. Importantly, disruption of Hsf2 caused a similar phenotype as the 2/3 deletion of MSYq, i.e., altered expression of the multicopy genes and increased mild sperm head abnormalities. Consequently, aberrant levels of chromatin packing proteins and more frequent DNA fragmentation were detected, implying that HSF2 is required for correct chromatin organization in the sperm. Our findings define a physiological role for HSF2 in the regulation of MSYq resident genes and the quality of sperm. chromatin packing ͉ heat shock factor ͉ MSYq ͉ promoter microarray ͉ spermatogenesis

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Section: Discussionmentioning

confidence: 99%

Promoter ChIP-chip analysis in mouse testis reveals Y chromosome occupancy by HSF2

Åkerfelt

Henriksson

Laiho

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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“…Low numbers of spermatozoa were recovered from the oviducts, and those with the most severely deformed heads were less frequent there than in the uterus. This clearly showed that a partial deletion of the Y chromosome affected the efficiency of fertilization and sperm morphology (Styrna et al, 1991;Styrna and Krzanowska, 1995;Styrna et al, 2002).…”

Section: Fig 2 Typical Examples Of Sperm Head Abnormalities (Accordmentioning

confidence: 85%

“…Experiments performed in the Department of Genetics and Evolution of the Jagiellonian University conducted by Krzanowska and her coworkers on inbred strains of mice, which differed in the level of sperm abnormalities and efficiency of fertilization, showed that the source of the Y chromosome plays a significant role in the inheritance of these traits and possibly contains genes controlling the course of spermatogenesis (Krzanowska, 1969;Krzanowska, 1972b;Krzanowska, 1986). This was confirmed later by the finding of an increased level of sperm abnormalities in males with a partial deletion of the Y chromosome (Styrna et al, 1991). These studies were the first ever description of the role of Y chromosome in determination of abnormal sperm heads.…”

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confidence: 93%

Genetic control of gamete quality in the mouse - a tribute to Halina Krzanowska

Styrna

2008

Int. J. Dev. Biol.

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In this article, we summarise the principal research findings of the distinguished Polish scientist, Professor Halina Krzanowska, related to the genetic control of mammalian gamete quality. During the early stages of her career, Halina Krzanowska conducted experiments on poultry and then she moved on to work on mice. All her research on gamete quality was conducted on the research models, consomic, congenic and recombinant inbred strains, which Krzanowska developed herself. These models differed mostly in their fertility. Krzanowska was one of the first researchers to demonstrate the influence of chromosome Y on the morphology of mice spermatozoa. She also showed that the uterotubal junction is in vivo a selection barrier for the morphologically abnormal spermatozoa, whereas in vitro abnormal spermatozoa are able to participate in fertilization, the function of selective barrier being performed by the granulosa cell layer and the zona pellucida. Another model which Krzanowska produced were chimaeras, which she used to find out if the percentage of abnormal spermatozoa and the efficiency of fertilization are determined by germ cells themselves or by environmental factors and she discovered that sperm head shape, the proportion of abnormal sperm and fertilizing capacity are determined mainly by the genotype of germ cells and only minimally by environmental factors.

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“…The BALB/c strain h a s a ve r y h ig h i n ci d en c e of ab n o r ma l s pe r m morphology and a significantly low capacitation competence in TYH [4,6], which suggest that the poor IVF competence of the BALB/c strain is due to poor sperm competence to capacitate in vitro. Other examples of poor fertilization in vitro have been shown in a mouse strain, B10.BR-Y d el , that has a partial deletion in the Y chromosome [23][24][25], and in the KE mouse strain [22,26]. In these strains, male-related factors were shown to be the major contributor to fertilization competence.…”

Section: Discussionmentioning

confidence: 99%

Kinetics of In Vitro Fertilization and Development of an Inbred Mouse Strain: A Study Comparing RFM/Ms with C57BL/6J

Kito¹,

Tateno

Ohta

et al. 2002

J.Mamm.Ova.Res.

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In vitro fer tilizability and subsequent developmental competence of inbred RFM/Ms mice, which have high incidence of myeloid leukemia by radiation, was studied. When response of RFM/Ms females to various amount of eCG (1.25-10.0 i.u.) In the past few decades, the development of techniques such as in vitro fertilization (IVF) and in vitro embryo culture (IVC) for many mammalian species has significantly contributed to clinical intervention in human and endangered species infertility, as well as the animal science of domesticated and laboratory animals [1]. Of these mammals, mice are most frequently used as models for reproductive physiological studies. Although many mouse strains have been bred for specific purposes of research, their reproductive profiles vary among strains [2]. In vitro handling, such as IVF and IVC is not necessarily possible in all inbred strains of mice [3][4][5][6]. The feasibility of IVF and IVC of a particular mouse strain has to be determined on a strain by strain basis in most cases.We report the ability to manipulate gametes and embryos from an inbred RFM/Ms mouse strain that has often been used in the study of leukemia [7,8]. In spite of the importance of this strain in radiation biology and immunology due to its high incidence of myeloid le uke m ia a ft er r adi at io n exp osu re [9,10 ], t he reproductive profile and gamete/zygote handling in vitro have not been studied. Therefore, we examined this strain's ovulatory response to hormone stimulation and the fertilization and developmental competence of RFM/ Ms gametes and zygotes in IVF and IVC including comparison with C57BL/6J gametes and embryos. As we observed differences in the kinetics of fertilization and embryo development, an attempt was made to investigate how paternal and maternal factors affect fertilization and developmental kinetics in vitro by producing hybrid embryos. Materials and Methods AnimalsInbred RFM/Ms and C57BL/6J mice have been maintained by sib mating over 80 generations in the specific pathogen free animal facility of the National

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An increased level of sperm abnormalities in mice with a partial deletion of the Y chromosome

Cited by 80 publications

References 6 publications

Promoter ChIP-chip analysis in mouse testis reveals Y chromosome occupancy by HSF2

Promoter ChIP-chip analysis in mouse testis reveals Y chromosome occupancy by HSF2

Genetic control of gamete quality in the mouse - a tribute to Halina Krzanowska

Kinetics of In Vitro Fertilization and Development of an Inbred Mouse Strain: A Study Comparing RFM/Ms with C57BL/6J

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