An Indigenous Biosurfactant Producing Burkholderia cepacia with High Emulsification Potential towards Crude Oil

Almatawah, Qadreyah

doi:10.4172/2161-0525.1000528

Cited by 9 publications

(2 citation statements)

References 39 publications

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“…8 ). The genus Burkholderia has been recognized for its ability to produce a stable biosurfactant with robust emulsification properties for crude oil, showcasing its potential for use in commercial bioremediation applications for oil-contaminated soils (Almatawah 2017 ). The obligate methylotrophs within the Methyloversatilis group have been noted for their role in the biodegradation of Benazolin-Ethyl in activated sludge and methane production (Cai et al 2011 ; Fenibo et al 2023b ).…”

Section: Discussionmentioning

confidence: 99%

Characterization and identification of long-chain hydrocarbon-degrading bacterial communities in long-term chronically polluted soil in Ogoniland: an integrated approach using culture-dependent and independent methods

Okoye,

Selvarajan,

Chikere

et al. 2024

Environ Sci Pollut Res

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Escalating oil consumption has resulted in an increase in accidental spills of petroleum hydrocarbons, causing severe environmental degradation, notably in vulnerable regions like the Niger Delta. Complex mixture of these hydrocarbons particularly long-chain alkanes presents unique challenges in restoration of polluted environment due to their chemical properties. This study aimed to investigate the long-chain hydrocarbon-degrading bacterial communities within long-term chronically polluted soil in Ogoniland, by utilizing both traditional cultivation methods and modern culture-independent techniques. Results revealed that surface-polluted soil (SPS) and subsurface soil (SPSS) exhibit significantly higher total organic carbon (TOC) ranging from 5.64 to 5.06% and total petroleum hydrocarbons (TPH) levels ranging from 36,775 ppm to 14,087 ppm, compared to unpolluted soil (UPS) with 1.97% TOC and 479 ppm TPH, respectively. Analysis of carbon chain lengths reveals the prevalence of longer-chain alkanes (C20-28) in the surface soil. Culture-dependent methods, utilizing crude oil enrichment (COE) and paraffin wax enrichment (PWE), yield 47 bacterial isolates subjected to a long-chain alkane degradation assay. Twelve bacterial strains demonstrate significant degradation abilities across all enriched media. Three bacterial members, namely Pseudomonas sp. (almA), Marinomonas sp. (almA), and Alteromonas (ladA), exhibit genes responsible for long-chain alkane degradation, demonstrating efficiency between 50 and 80%. Culture-independent analysis reveals that surface SPS samples exhibit greater species richness and diversity compared to subsurface SPSS samples. Proteobacteria dominates as the phylum in both soil sample types, ranging from 22.23 to 82.61%, with Firmicutes (0.2–2.22%), Actinobacteria (0.4–3.02%), and Acidobacteria (0.1–3.53%) also prevalent. Bacterial profiles at genus level revealed that distinct variations among bacterial populations between SPS and SPSS samples comprising number of hydrocarbon degraders and the functional predictions also highlight the presence of potential catabolic genes (nahAa, adh2, and cpnA) in the polluted soil. However, culture-dependent analysis only captured a few of the dominant members found in culture-independent analysis, implying that more specialized media or environments are needed to isolate more bacterial members. The findings from this study contribute valuable information to ecological and biotechnological aspects, aiding in the development of more effective bioremediation applications for restoring oil-contaminated environments.

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Section: Discussionmentioning

confidence: 99%

Characterization and identification of long-chain hydrocarbon-degrading bacterial communities in long-term chronically polluted soil in Ogoniland: an integrated approach using culture-dependent and independent methods

Okoye,

Selvarajan,

Chikere

et al. 2024

Environ Sci Pollut Res

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“…La tensión superficial se midió con un tensiómetro Dataphysics modelo DCAT 11 equipado con un anillo de Du Noüy (Almatawah, 2017). Las muestras se prepararon a una concentración de 5 g/L y se disolvieron en MeOH, se añadieron alícuotas de 100, 200, 300 y 500 µL.…”

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Producción de biosurfactantes en cultivo en medio sólido

Sánchez

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Glycolipids biosurfactants production using low-cost substrates for environmental remediation: progress, challenges, and future prospects

Aqif,

Shah,

Khan

et al. 2024

Environ Sci Pollut Res

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An Indigenous Biosurfactant Producing Burkholderia cepacia with High Emulsification Potential towards Crude Oil

Cited by 9 publications

References 39 publications

Characterization and identification of long-chain hydrocarbon-degrading bacterial communities in long-term chronically polluted soil in Ogoniland: an integrated approach using culture-dependent and independent methods

Characterization and identification of long-chain hydrocarbon-degrading bacterial communities in long-term chronically polluted soil in Ogoniland: an integrated approach using culture-dependent and independent methods

Producción de biosurfactantes en cultivo en medio sólido

Glycolipids biosurfactants production using low-cost substrates for environmental remediation: progress, challenges, and future prospects

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