An Indigenous, Field-Deployable, Lateral Flow Immunochromatographic Assay Rapidly Detects Infectious Myonecrosis in Shrimp, Litopenaeus vannamei

Kumar, Shanmugam Santhosh; Jamalpure, Snehal; Ahmed, A Nafeez; Taju, G.; Vimal, S.; Majeed, Shahid; Suryakodi, S.; Syed, Rabbani; Paknikar, Kishore M.; Rajwade, Jyutika M.; Hameed, A.S. Sahul

doi:10.1007/s10126-022-10172-6

Cited by 1 publication

(1 citation statement)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…furthermore it was mentioned that the lateral flow devices sensitivity for E. coli O157: H7 bovine feces reached 10 5 CFU/g (Jung et al 2005). Also, another study was investigated an increase in LFD strips sensitivity for detection of Vibrio harveyi of 1-10CFU/mL in the tested sample (Sithigorngul et al 2007;Kumar et al 2022;Huda et al 2022). The prepared P. multocida LFK when compared to conventional bacteriological tests were analyzed and were recorded as sensitivity, specificity and accuracy test were 90.4, 94.4 and 93%, respectively, while M. haemolytica kits showed 84.7, 94, and 91.5%, respectively.…”

Section: Discussionmentioning

confidence: 99%

Diagnosis of Pasteurella Multocida and Mannheimia Haemolytica Infections in Cattle Using Lateral Flow Immunochromatographic Assay

2023

Int J Vet Sci

View full text Add to dashboard Cite

This study was mainly designed to advance and recover the control measures of Bovine respiratory disease (BRD) via accelerating the field identification of some BRD causing pathogens. A simple rapid field test was developed mainly to identify Pasteurella multocida and Mannheimia haemolytica, both are highly associated with BRD. A full identified Pasteurella multocida and Mannheimia haemolytica cultures were earned from Microbiology Department, Faculty of Veterinary Medicine, Cairo University, Egypt. Species-specific bacterial antigen of outer membrane protein of each bacterial strain was prepared separately for M. haemolytica and P. multocida. Specific polyclonal antibodies were prepared by amplifying each one in guinea pigs and rabbits. Pathogens-specific lgG were purified using caprylic acid technique. Pathogens-specific lgG titers were measured by ELISA. The colloidal gold nanoparticles were conjugated to the rabbit mono specific polyclonal lgG antibody and did not conjugate to guinea pig specific polyclonal lgG. The particles were then utilized to develop the Lateral flow kits (LFK) which is a rapid assay for detection for P. multocida and M. haemolytica in clinical samples when Compared to the conventional Bacteriological tests. Results: Percentage of accuracy, specificity and sensitivity of LFK kits recorded 93, 94.4 and 90.4%, respectively, for P. multocida and 91.5, 94.3and 84.7%, respectively, for Mannheimia haemolytica.

show abstract

Section: Discussionmentioning

confidence: 99%

Diagnosis of Pasteurella Multocida and Mannheimia Haemolytica Infections in Cattle Using Lateral Flow Immunochromatographic Assay

2023

Int J Vet Sci

View full text Add to dashboard Cite

show abstract

An Indigenous, Field-Deployable, Lateral Flow Immunochromatographic Assay Rapidly Detects Infectious Myonecrosis in Shrimp, Litopenaeus vannamei

Cited by 1 publication

References 51 publications

Diagnosis of Pasteurella Multocida and Mannheimia Haemolytica Infections in Cattle Using Lateral Flow Immunochromatographic Assay

Diagnosis of Pasteurella Multocida and Mannheimia Haemolytica Infections in Cattle Using Lateral Flow Immunochromatographic Assay

Contact Info

Product

Resources

About