An industrial perspective fermentative bioreduction of aromatic ketones by Penicillium rubens VIT SS1 and Penicillium citrinum VIT SS2

Abstract

Isolation and characterization of fungal cultures: Isolation and morphological identificationThe fungal cultures were isolated from soil using serial dissolution and selective spread plating in PDA, MEA and SDA. A single colony picked and streaked on PDA, MEA and SDBA after serial dissolution. Single purified colony inoculated to liquid media, PDB & SDA and incubated for 3 days at 25°C, 200 rpm. Grown cultures were streaked in PDB plates, after sporulation, preserved for the study.

Alcohol Dehydrogenases with anti‐Prelog Stereopreference in Synthesis of Enantiopure Alcohols

“…Asymmetric reduction reactions of prochiral ketones in anti ‐Prelog mode have been reported using whole cells of Acetobacter pasteurianus GIM1.158, [44] Leifsonia xyli HS0904, [45] Leifsonia xyli CCTCCM 2010241, [46] Lactobacillus reuteri DSM 20016, [47] and Penicillium citrinum VIT SS2, [48] and also when using the fungus Penicillium expansum . [49] The use of whole cells is attractive for several reasons, including production cost, space‐time‐yield and easier procedures.…”