An Influenza Hemagglutinin a Peptide Assay Based on the Enzyme-Multiplied Immunoassay Technique

Chiu, May L.; Lai, Denton; Monbouquette, Harold G.

doi:10.1080/15321819.2011.538623

Cited by 3 publications

(1 citation statement)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…So far, EMIT has already attracted a lot of research interests in chemical analysis, especially in therapeutic drug monitoring [12] and drugs of abuse testing [14]. However, a serious decrease in the activity of reporter enzyme (up to 90%) was observed after labeling with hapten/analyte [15,16], which blocked the development of EMIT assay. Therefore, it is necessary to investigate the parameters influencing the enzyme activity.…”

Section: Fig 1 Scheme Of (A) Cg-g6pd Activity and (B) The Repression Of Cg-g6pd Activity Upon Cg Antibody Bindingmentioning

confidence: 99%

Fabricating cholyglycine–glucose‐6‐phosphate dehydrogenase conjugates for cholyglycine detection

Yang

Zhang

Zhi

et al. 2019

Biotech and App Biochem

View full text Add to dashboard Cite

To establish cholyglycine (CG) detection via enzyme‐multiplied immunoassay technique (EMIT), glucose‐6‐phosphate dehydrogenase (G6PD) was used as a reporter enzyme to prepare hapten–enzyme conjugate. Gel electrophoresis and UV scanning demonstrated that G6PD was successfully labeled with cholyglycine, and CG‐G6PD conjugate was obtained. Furthermore, the effects of various parameters on the preparation of CG‐G6PD conjugates were investigated. Consequently, CG amount, nicotinamide adenine dinucleotide, d‐glucose‐6‐phosphate (G6P), phosphate buffer and the pH, and ionic strength of solution had important effects on the residual activity of CG‐G6PD. Moreover, CG amount, the pH, and G6P played important roles in changing CG labeling location on G6PD. Using the CG‐G6PD conjugate as test kit, the cholyglycine–EMIT calibration curve was established, which could be employed in clinical detection of cholyglycine. This study provides some valuable information for preparing hapten–G6PD conjugates.

show abstract

Section: Fig 1 Scheme Of (A) Cg-g6pd Activity and (B) The Repression Of Cg-g6pd Activity Upon Cg Antibody Bindingmentioning

confidence: 99%

Fabricating cholyglycine–glucose‐6‐phosphate dehydrogenase conjugates for cholyglycine detection

Yang

Zhang

Zhi

et al. 2019

Biotech and App Biochem

View full text Add to dashboard Cite

show abstract

Withdrawal: Yang, M.‐N., Zhang, Y.‐F., Zhi, G.‐Y., Gu, X.‐F., Han, L. and Zhang, D‐H. (2020), Fabricating cholyglycine‐glucose‐6‐phosphate dehydrogenase conjugates for cholyglycine detection. Biotechnology and Applied Biochemistry. (Accepted Article): https://doi.org/10.1002/bab.1983.

Yang

Zhang

Zhi

et al. 2024

Biotech and App Biochem

View full text Add to dashboard Cite

To establish cholyglycine (CG) detection via enzyme multiplied immunoassay technique (EMIT), glucose‐6‐phosphate dehydrogenase (G6PD) was used as a reporter enzyme to prepare hapten‐enzyme conjugate. Gel electrophoresis and UV scanning demonstrated that G6PD was successfully labeled with cholyglycine and CG‐G6PD conjugate was obtained. Furthermore, the effects of various parameters on the preparation of CG‐G6PD conjugates were investigated. Consequently, CG amount, NADH, D‐glucose‐6‐phosphate (G6P), phosphate buffer and the pH, and ionic strength of solution had important effects on the residual activity of CG‐G6PD. Moreover, CG amount, the pH, and G6P played important roles in changing CG labeling location on G6PD. Using the CG‐G6PD conjugate as test kit, the cholyglycine‐EMIT calibration curve was established, which could be employed in clinical detection of cholyglycine. This study provides some valuable information for preparing hapten‐G6PD conjugates.This article is protected by copyright. All rights reserved

show abstract

Mathematical modeling of bioassays

Sotnikov

Zherdev

Dzantiev

2017

Biochemistry Moscow

View full text Add to dashboard Cite

The high affinity and specificity of biological receptors determine the demand for and the intensive development of analytical systems based on use of these receptors. Therefore, theoretical concepts of the mechanisms of these systems, quantitative parameters of their reactions, and relationships between their characteristics and ligand-receptor interactions have become extremely important. Many mathematical models describing different bioassay formats have been proposed. However, there is almost no information on the comparative characteristics of these models, their assumptions, and predictive insights. In this review we suggested a set of criteria to classify various bioassays and reviewed classical and contemporary publications on these bioassays with special emphasis on immunochemical analysis systems as the most common and in-demand techniques. The possibilities of analytical and numerical modeling are discussed, as well as estimations of the minimum concentrations that may be detected in bioassays and recommendations for the choice of assay conditions.

show abstract

An Influenza Hemagglutinin a Peptide Assay Based on the Enzyme-Multiplied Immunoassay Technique

Cited by 3 publications

References 24 publications

Fabricating cholyglycine–glucose‐6‐phosphate dehydrogenase conjugates for cholyglycine detection

Fabricating cholyglycine–glucose‐6‐phosphate dehydrogenase conjugates for cholyglycine detection

Mathematical modeling of bioassays

Contact Info

Product

Resources

About