An inhibition of ceruloplasmin expression induced by cerebral ischemia in the cortex and hippocampus of rats

Li, Yanwei; Lin, Li; Zhao, Jinying

doi:10.1007/s12264-008-1017-2

Cited by 4 publications

(2 citation statements)

References 22 publications

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“…Finally, some reports suggest that ATP7B might be involved in the synthesis of coagulation factors VIII and V [135,136]. Besides the liver, ATP7B expression has been detected in cells of neural origin and vascular endothelial cells [137,138]. It has been assumed that segments of the luminal loops of ATP7B participate in the direct transfer of copper to the active sites of intact Cp [139,140].…”

Section: Interplay Between Silver and Pathways Driving Intracellulmentioning

confidence: 99%

Silver Ions as a Tool for Understanding Different Aspects of Copper Metabolism

et al. 2019

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In humans, copper is an important micronutrient because it is a cofactor of ubiquitous and brain-specific cuproenzymes, as well as a secondary messenger. Failure of the mechanisms supporting copper balance leads to the development of neurodegenerative, oncological, and other severe disorders, whose treatment requires a detailed understanding of copper metabolism. In the body, bioavailable copper exists in two stable oxidation states, Cu(I) and Cu(II), both of which are highly toxic. The toxicity of copper ions is usually overcome by coordinating them with a wide range of ligands. These include the active cuproenzyme centers, copper-binding protein motifs to ensure the safe delivery of copper to its physiological location, and participants in the Cu(I) ↔ Cu(II) redox cycle, in which cellular copper is stored. The use of modern experimental approaches has allowed the overall picture of copper turnover in the cells and the organism to be clarified. However, many aspects of this process remain poorly understood. Some of them can be found out using abiogenic silver ions (Ag(I)), which are isoelectronic to Cu(I). This review covers the physicochemical principles of the ability of Ag(I) to substitute for copper ions in transport proteins and cuproenzyme active sites, the effectiveness of using Ag(I) to study copper routes in the cells and the body, and the limitations associated with Ag(I) remaining stable in only one oxidation state. The use of Ag(I) to restrict copper transport to tumors and the consequences of large-scale use of silver nanoparticles for human health are also discussed.

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Section: Interplay Between Silver and Pathways Driving Intracellulmentioning

confidence: 99%

Silver Ions as a Tool for Understanding Different Aspects of Copper Metabolism

et al. 2019

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“…2). Immunohistochemical studies of Cp in brain sections has previously identified expression in both cell types, 39 and it is unclear as to why cultured astrocytes do not display a Cu peak corresponding to Cp. A variety of Cu-60 binding proteins and peptides have been identified in astrocytes, including transporters (Copper transporter receptor 1 -Ctr1; divalent metal transporter 1 -DMT1; ATP7A), intracellular traffickers (SOD-1, copper chaperone for superoxide dismutase -CSS; ATOX1, Cox17), and storage and detoxifiers (metallothioneins -MTs).…”

Section: Diversity In Metalloproteins Between Cell Typesmentioning

confidence: 99%

Profiling the iron, copper and zinc content in primary neuron and astrocyte cultures by rapid online quantitative size exclusion chromatography-inductively coupled plasma-mass spectrometry

et al. 2013

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Metals often determine the chemical reactivity of the proteins to which they are bound. Each cell in the body tightly maintains a unique metalloproteomic profile, mostly dependent on function. This paper describes an analytical online flow injection quantitative size exclusion chromatography-inductively coupled plasma-mass spectrometry (SEC-ICP-MS) method, which was applied to profiling the metal-binding proteins found in primary cultures of neurons and astrocytes. This method can be conducted using similar amounts of sample to those used for Western blotting (20-150 μg protein), and has a turnaround time of <15 minutes. Metalloprotein standards for Fe (as ferritin), Cu and Zn (as superoxide dismutase-1) were used to construct multi-point calibration curves for online quantification of metalloproteins by SEC-ICP-MS. Homogenates of primary neuron and astrocyte cultures were analysed by SEC-ICP-MS. Online quantification by external calibration with metalloprotein standards determined the mass of metal eluting from the column relative to time (as pg s(-1)). Total on-column Fe, Cu and Zn detection limits ranged from 0.825 ± 0.005 ng to 13.6 ± 0.7 pg. Neurons and astrocytes exhibited distinct metalloprotein profiles, featuring both ubiquitous and unique metalloprotein species. Separation and detection by SEC-ICP-MS allows appraisal of these metalloproteins in their native state, and online quantification was achieved using this relatively simple external calibration process.

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Multicopper oxidases: an innovative approach for oxygen management of aerobic organisms

Arrigoni

2009

Rend. Fis. Acc. Lincei

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Multicopper oxidases (MCOs), such as ascorbic acid oxidase and ceruloplasmin, are multidomain proteins capable of oxidizing many structurally unrelated compounds reducing oxygen to water without ever generating reactive oxygen species. While MCOs show great oxidative versatility, they can only transfer electrons to molecular oxygen, which is the obligate electron acceptor. Therefore, MCOs could also be considered as ''O 2 consuming enzymes'', thus contributing to create those states of hypoxia that are normally found in tissues, cells and cell compartments. Since hypoxia is also a common feature of many rapidly growing solid tumors, we postulate that the regulation of GPI-ceruloplasmin isoform, present on the surface of the plasma membrane, could be the molecular event in the creation and the maintenance of hypoxia in tumor cells. By silencing the different MCO genes with siRNA, it would appear possible to attempt to overcome tumor hypoxia, thus improving the efficiency of radiotherapy.

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An inhibition of ceruloplasmin expression induced by cerebral ischemia in the cortex and hippocampus of rats

Cited by 4 publications

References 22 publications

Silver Ions as a Tool for Understanding Different Aspects of Copper Metabolism

Silver Ions as a Tool for Understanding Different Aspects of Copper Metabolism

Profiling the iron, copper and zinc content in primary neuron and astrocyte cultures by rapid online quantitative size exclusion chromatography-inductively coupled plasma-mass spectrometry

Multicopper oxidases: an innovative approach for oxygen management of aerobic organisms

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