An initial assessment of genetic diversity for Phytophthora capsici in northern and central Mexico

Castro-Rocha, Arturo; Shrestha, Sandesh; Lyon, Becky; Grimaldo-Pantoja, Graciela Lizette; Flores-Marges, Juan Pedro; Valero-Galván, José; Aguirre-Ramírez, Marisela; Osuna-Ávila, Pedro; Gómez-Dorantes, Nuria; Ávila-Quezada, Graciela Dolores; Luna‐Ruíz, José de Jesús; Rodríguez-Alvarado, Gerardo; Fernández-Pavía, S. P.; Lamour, Kurt

doi:10.1007/s11557-016-1157-0

Cited by 27 publications

(26 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In North America and Mexico, P. capsici often undergoes meiosis through the interaction of an A1 and A2 mating type to produce thick-walled, long-lived sexual dormancy spores (oospores) [25][26][27]. Yet, newly introduced populations, even those emanating from a small focus of initial infection (e.g.…”

Section: Dynamic Extreme Aneuploidy Phytophthora Capsicimentioning

confidence: 99%

Dynamic Extreme Aneuploidy (DEA) in the vegetable pathogen Phytophthora capsici and the potential for rapid asexual evolution

Shrestha

Zhou

et al. 2020

PLoS ONE

Self Cite

View full text Add to dashboard Cite

Oomycete plant pathogens are difficult to control and routine genetic research is challenging. A major problem is instability of isolates. Here we characterize >600 field and single zoospore isolates of Phytophthora capsici for inheritance of mating type, sensitivity to mefenoxam, chromosome copy number and heterozygous allele frequencies. The A2 mating type was highly unstable with 26% of 241 A2 isolates remaining A2. The A1 mating type was stable. Isolates intermediately resistant to mefenoxam produced fully resistant single-spore progeny. Sensitive isolates remained fully sensitive. Genome re-sequencing of single zoospore isolates revealed extreme aneuploidy; a phenomenon dubbed Dynamic Extreme Aneuploidy (DEA). DEA is characterized by the asexual inheritance of diverse intra-genomic combinations of chromosomal ploidy ranging from 2N to 3N and heterozygous allele frequencies that do not strictly correspond to ploidy. Isolates sectoring on agar media showed dramatically altered heterozygous allele frequencies. DEA can explain the rapid increase of advantageous alleles (e.g. drug resistance), mating type switches and copy neutral loss of heterozygosity (LOH). Although the mechanisms driving DEA are unknown, it can play an important role in adaptation and evolution and seriously hinders all aspects of P. capsici research.

show abstract

Section: Dynamic Extreme Aneuploidy Phytophthora Capsicimentioning

confidence: 99%

Dynamic Extreme Aneuploidy (DEA) in the vegetable pathogen Phytophthora capsici and the potential for rapid asexual evolution

Shrestha

Zhou

et al. 2020

PLoS ONE

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, P. capsici is one of the most genetically diverse and plastic eukaryotic microorganisms yet described. Recently, the phenotypic and genotypic diversity of P. capsici populations has been noted at a global scale within countries and at a local scale within regions using a wide range of molecular markers (Castro‐Rocha et al, ; Hulvey et al, ; Islam, Babadoost, Lambert, Ndeme, & Fouly, ; Lamour & Hausbeck,; Silvar, Merino, & Díaz, ; Sun, Jia, O'Neill, Feng, & Zhang, ). In Peru, Argentina, eastern and northern China, long‐lived clonal lineages were detected across wide geographical areas and can survive for a long time (Gobena, McGrath, & Lamour, ; Hu et al, ; Hurtado‐González, Aragón‐Caballero, Apaza‐Tapia, Donahoo, & Lamour, ; Li et al, ; Sun et al, ).…”

Section: Introductionmentioning

confidence: 99%

Investigation of the genetic diversity of Phytophthora capsici in China using a universal fluorescent labelling method

Chen

Zhang

Huang

et al. 2018

Journal of Phytopathology

View full text Add to dashboard Cite

Phytophthora capsici is an important oomycete pathogen threatening the vegetable production in China, but very little is known about its population structure. The objective of the present study was to evaluate the genetic diversity of 49 P. capsici isolates obtained from 2007 to 2014 at nine provincial locations in China. Isolates were assessed for mating type, metalaxyl resistance and simple sequence repeat (SSR) genotype. Mating‐type analyses of the isolates showed that both mating types were present in all of the sampled production regions, and the mating‐type frequency in the total Chinese population did not deviate significantly from a 1:1 ratio. Responses of isolates to the fungicide metalaxyl indicated the presence of intermediate resistance to metalaxyl among the field population. A universal fluorescent labelling method was adapted in this study to improve the efficiency of SSR genotyping. Microsatellite genotyping of the isolates using seven SSR markers revealed 44 unique multilocus genotypes. Genetic analyses indicated the existence of two genetic clusters within Chinese P. capsici collection. Clonal reproduction may play a more prominent role in Yunnan Province, but non‐existence of repeated genotypes and existence of both mating types throughout all regions suggest outcrossing and sexual recombination likely play an important role in the overall epidemiology in China. Future studies would include expanded scale sampling at single regions over multiple years to better define the genetic diversity of P. capsici in China.

show abstract

“…This represents a challenge for producers because genetic recombination can lead to the emergence of strains capable of breaking the resistance barrier of the hosts (Jiang et al, 2015). The high genetic diversity of P. capsici in the center of Mexico (Castro-Rocha et al, 2016) and the high diversity of the Capsicum genus in this country (Aguilar-Mel endez et al, 2009) may drive the evolution of diverse virulence phenotypes in Michoac an. It is necessary to know the virulence phenotype makeup of the P. capsici populations in the production fields to be successful in the development of resistant cultivars (Jiang et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

“…In Mexico, there are no reports on the presence or characterization of virulence phenotypes of isolates of P. capsici recovered from production zones. Due to the high genetic diversity of this pathogen in Mexico (Castro-Rocha et al, 2016), it is likely that multiple virulence phenotypes exist in field populations. The objective of this study was to characterize the virulence phenotypes for P. capsici isolates recovered from Solanaceae and Cucurbitaceae production fields in Michoac an, Mexico using the NMRILs.…”

mentioning

confidence: 99%

Virulence Phenotypes on Chili Pepper for Phytophthora capsici Isolates from Michoacán, Mexico

Reyes-Tena¹,

Castro-Rocha²,

Rodríguez-Alvarado³

et al. 2019

horts

Self Cite

View full text Add to dashboard Cite

Phytophthora blight of vegetables caused by Phytophthora capsici causes significant economic losses in production of Solanaceae and Cucurbitaceae crops in Mexico. The development of universal resistant chili pepper cultivars is challenging due to the diverse virulence phenotypes produced by P. capsici. The objective of the study was to characterize the diversity of phenotypic interactions for P. capsici isolates recovered from production fields in Michoacán, Mexico, to facilitate the development of resistant cultivars. Virulence phenotypes were characterized for 12 isolates of P. capsici using 26 Capsicum annuum New Mexico Recombinant Inbred Lines (NMRILs) in greenhouse conditions. Criollo de Morelos CM-334 and California Wonder were used as resistant and susceptible controls, respectively. Seedlings at the four to eight true leaf stage were inoculated with 10,000 zoospores per seedling and disease severity was evaluated at 20 days post-inoculation. Two of the P. capsici isolates did not infect any pepper host even though the isolate was less than a year old. The 10 virulent isolates were designated in 10 virulence phenotypes. The information generated by this study is of utmost importance for efforts of producing resistant cultivars specific for Michoacán producers.

show abstract

An initial assessment of genetic diversity for Phytophthora capsici in northern and central Mexico

Cited by 27 publications

References 19 publications

Dynamic Extreme Aneuploidy (DEA) in the vegetable pathogen Phytophthora capsici and the potential for rapid asexual evolution

Dynamic Extreme Aneuploidy (DEA) in the vegetable pathogen Phytophthora capsici and the potential for rapid asexual evolution

Investigation of the genetic diversity of Phytophthora capsici in China using a universal fluorescent labelling method

Virulence Phenotypes on Chili Pepper for Phytophthora capsici Isolates from Michoacán, Mexico

Contact Info

Product

Resources

About